Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M4S28_RS15270 Genome accession   NZ_CP097293
Coordinates   3418584..3419621 (-) Length   345 a.a.
NCBI ID   WP_004735478.1    Uniprot ID   A0A1R3EAA6
Organism   Vibrio sp. J383     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3416697..3427170 3418584..3419621 within 0


Gene organization within MGE regions


Location: 3416697..3427170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4S28_RS15255 (M4S28_15255) ruvX 3416697..3417119 (+) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  M4S28_RS15260 (M4S28_15260) - 3417219..3417389 (+) 171 WP_249632056.1 hypothetical protein -
  M4S28_RS15265 (M4S28_15265) pilU 3417460..3418569 (-) 1110 WP_102542782.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M4S28_RS15270 (M4S28_15270) pilT 3418584..3419621 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  M4S28_RS15275 (M4S28_15275) - 3419648..3420352 (+) 705 WP_061025919.1 YggS family pyridoxal phosphate-dependent enzyme -
  M4S28_RS15280 (M4S28_15280) proC 3420465..3421283 (+) 819 WP_017077774.1 pyrroline-5-carboxylate reductase -
  M4S28_RS15285 (M4S28_15285) - 3421359..3421916 (+) 558 WP_004735475.1 YggT family protein -
  M4S28_RS15290 (M4S28_15290) yggU 3421946..3422236 (+) 291 WP_009847703.1 DUF167 family protein YggU -
  M4S28_RS15295 (M4S28_15295) - 3422893..3423498 (+) 606 WP_249632057.1 hypothetical protein -
  M4S28_RS15300 (M4S28_15300) - 3424008..3424418 (-) 411 WP_249632058.1 hypothetical protein -
  M4S28_RS15305 (M4S28_15305) - 3424415..3424843 (-) 429 WP_249632740.1 M15 family metallopeptidase -
  M4S28_RS15310 (M4S28_15310) - 3424917..3425600 (-) 684 WP_249632059.1 hypothetical protein -
  M4S28_RS15315 (M4S28_15315) - 3425600..3426250 (-) 651 WP_186171714.1 hypothetical protein -
  M4S28_RS15320 (M4S28_15320) - 3426247..3427170 (-) 924 WP_249632060.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38317.83 Da        Isoelectric Point: 6.0512

>NTDB_id=688335 M4S28_RS15270 WP_004735478.1 3418584..3419621(-) (pilT) [Vibrio sp. J383]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPEIFERISNYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=688335 M4S28_RS15270 WP_004735478.1 3418584..3419621(-) (pilT) [Vibrio sp. J383]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGCCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGCGGTGAATTTGAAGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAACGTTGGTCGT
TTCCGTGTTAATGCTTTTAACCAATCTCGTGGCTGCTCTGCTGTGTTCCGTACCATTCCAGTAGAGATTCCCACCCTTGA
ACAGCTAGGGGCTCCGGAGATCTTTGAAAGAATATCTAATTACGAAAAAGGGCTAGTATTGGTTACTGGGCCGACTGGTT
CAGGTAAGTCGACAACTTTGGCTGCTATGGTAGATTACGTTAACCGTAACCATAATAAACATATATTGACGATTGAAGAC
CCGATCGAATTTGTTCATACCAACAACAAATGCCTAATCAACCAACGAGAAGTGCACCGCGATACTCACAGCTTTAAAGC
GGCGTTGCGCAGTGCGTTACGTGAAGACCCAGACGTAATCCTTGTCGGCGAACTTCGTGACCAAGAGACGATTAGCTTAG
CGCTAACCGCAGCAGAAACTGGCCACTTAGTTTTTGGTACCTTGCATACTAGCTCTGCGGCAAAAACCATAGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGTTGTCTGAATCGTTACGTTCGGTGATTGCCCAGAA
GCTGTTAAAGCGTGTTGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCGATCAGAAACTTGATCC
GTGAAGACAAGGTCGCGCAGATGTATTCGATCATTCAAACGGGCGCGGCACACGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGCTGATGGCACAAGGCTTAGTTGATTCAGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R3EAA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Pseudomonas aeruginosa PAK

69.412

98.551

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368