Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M4S28_RS15265 Genome accession   NZ_CP097293
Coordinates   3417460..3418569 (-) Length   369 a.a.
NCBI ID   WP_102542782.1    Uniprot ID   -
Organism   Vibrio sp. J383     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3416697..3427170 3417460..3418569 within 0


Gene organization within MGE regions


Location: 3416697..3427170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4S28_RS15255 (M4S28_15255) ruvX 3416697..3417119 (+) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  M4S28_RS15260 (M4S28_15260) - 3417219..3417389 (+) 171 WP_249632056.1 hypothetical protein -
  M4S28_RS15265 (M4S28_15265) pilU 3417460..3418569 (-) 1110 WP_102542782.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M4S28_RS15270 (M4S28_15270) pilT 3418584..3419621 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  M4S28_RS15275 (M4S28_15275) - 3419648..3420352 (+) 705 WP_061025919.1 YggS family pyridoxal phosphate-dependent enzyme -
  M4S28_RS15280 (M4S28_15280) proC 3420465..3421283 (+) 819 WP_017077774.1 pyrroline-5-carboxylate reductase -
  M4S28_RS15285 (M4S28_15285) - 3421359..3421916 (+) 558 WP_004735475.1 YggT family protein -
  M4S28_RS15290 (M4S28_15290) yggU 3421946..3422236 (+) 291 WP_009847703.1 DUF167 family protein YggU -
  M4S28_RS15295 (M4S28_15295) - 3422893..3423498 (+) 606 WP_249632057.1 hypothetical protein -
  M4S28_RS15300 (M4S28_15300) - 3424008..3424418 (-) 411 WP_249632058.1 hypothetical protein -
  M4S28_RS15305 (M4S28_15305) - 3424415..3424843 (-) 429 WP_249632740.1 M15 family metallopeptidase -
  M4S28_RS15310 (M4S28_15310) - 3424917..3425600 (-) 684 WP_249632059.1 hypothetical protein -
  M4S28_RS15315 (M4S28_15315) - 3425600..3426250 (-) 651 WP_186171714.1 hypothetical protein -
  M4S28_RS15320 (M4S28_15320) - 3426247..3427170 (-) 924 WP_249632060.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41381.27 Da        Isoelectric Point: 5.8700

>NTDB_id=688334 M4S28_RS15265 WP_102542782.1 3417460..3418569(-) (pilU) [Vibrio sp. J383]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNAAQVAQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMTKSKEIGMQTFDQALY
DLVLAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=688334 M4S28_RS15265 WP_102542782.1 3417460..3418569(-) (pilU) [Vibrio sp. J383]
ATGGAATTGAATCAAATCCTTGAAGGGATGCTATCTCAAAAAGCATCGGATCTTTATATCACCGTTGATGCGCCTATCCT
GTTTCGTGTCGATGGTGAACTGCGACCTCAAGGCGAGAAATTGAATGCGGCTCAGGTTGCTCAGTTACTTGATGCGATGA
TGGATCAAGACCGACGCGATGAATATCAGCAAACGCGAGAGGCTAATTTTGCCATAGTGCGTGATTTTGGTCGTTTTCGT
GTGAGTGCGTTTTTTCAGCGAGAGCTACCAGGAGCGGTCATTCGTCGTATCGAAACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTGCTGCAAGACCTTTCAATCGCTAAGCGCGGACTTGTGCTGGTGGTTGGGGCAACAGGTTCTGGTA
AATCAACCTCGATGGCCGCAATGACAGGCTATCGCAATACCAATCGCTCAGGGCATATTTTGACGGTTGAAGATCCGATT
GAATTCGTACACGAACATAAAAAGTGCATCGTGACACAACGTGAGGTCGGGCTCGACACCGAGAGTTACGAAGTTGCGCT
TAAGAACTCTTTACGCCAAGCACCGGATATGATATTGATTGGCGAAATCCGTAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGCCACTTGTGTATGGCAACACTGCACGCGAATAATGCCAACCAAGCGTTAGAACGTATTCTCCAT
TTGGTACCGAAGGAACAAAAAGAACAGTTCTTATTTGATCTGTCGATGAACCTGCGTGGTGTGGTCGCTCAGCAATTAAT
CCGAGATAAGAATGGCAGCGGTCGCCATGGTGTATTCGAGATTCTGCTGAATAGCCCAAGAGTGTCTGATTTGATTCGTC
GTGGTGAGTTGCATGAACTAAAAGCGACTATGACTAAATCGAAAGAGATTGGTATGCAGACCTTTGACCAAGCGTTGTAT
GACTTGGTGCTTGCAGGCAAGATCAGTGAAGAAGACGCATTCCACAGTGCTGATTCCGCGAATGACTTACGCTTAATGCT
AAAAACCAGACGTGGTGATGATGACTACGGAACCGGTGCTTTGGCTGGCGTGAAGATAGATATGGGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

99.729

0.84
  pilU Pseudomonas stutzeri DSM 10701

56.065

100

0.564
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Acinetobacter baumannii strain A118

41.813

92.683

0.388
  pilT Acinetobacter baumannii D1279779

41.813

92.683

0.388
  pilT Acinetobacter nosocomialis M2

41.813

92.683

0.388
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Acinetobacter baylyi ADP1

42.169

89.973

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363