Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M4R69_RS22155 Genome accession   NZ_CP097198
Coordinates   4569939..4570919 (+) Length   326 a.a.
NCBI ID   WP_085455522.1    Uniprot ID   -
Organism   Escherichia coli strain 2-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4564939..4575919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4R69_RS22125 (M4R69_22120) yggM 4565544..4566551 (+) 1008 WP_000745232.1 DUF1202 family protein -
  M4R69_RS22130 (M4R69_22125) hemW 4566616..4567752 (-) 1137 WP_044706006.1 radical SAM family heme chaperone HemW -
  M4R69_RS22135 (M4R69_22130) rdgB 4567745..4568338 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  M4R69_RS22140 (M4R69_22135) yggU 4568346..4568636 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  M4R69_RS22145 (M4R69_22140) yggT 4568633..4569199 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  M4R69_RS22150 (M4R69_22145) yggS 4569217..4569921 (-) 705 WP_000997807.1 pyridoxal phosphate homeostasis protein -
  M4R69_RS22155 (M4R69_22150) pilT 4569939..4570919 (+) 981 WP_085455522.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M4R69_RS22160 (M4R69_22155) ruvX 4571093..4571509 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  M4R69_RS22165 (M4R69_22160) yqgE 4571509..4572072 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  M4R69_RS22170 (M4R69_22165) gshB 4572181..4573131 (-) 951 WP_000593259.1 glutathione synthase -
  M4R69_RS22175 (M4R69_22170) rsmE 4573144..4573875 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M4R69_RS22180 (M4R69_22175) endA 4573955..4574662 (-) 708 WP_001327408.1 deoxyribonuclease I -
  M4R69_RS22185 (M4R69_22180) yggI 4574757..4575254 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36073.34 Da        Isoelectric Point: 5.9975

>NTDB_id=687722 M4R69_RS22155 WP_085455522.1 4569939..4570919(+) (pilT) [Escherichia coli strain 2-1]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPLILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=687722 M4R69_RS22155 WP_085455522.1 4569939..4570919(+) (pilT) [Escherichia coli strain 2-1]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACTGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGTAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
TGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTACGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGTTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.906

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362