Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M1L61_RS05205 Genome accession   NZ_CP096975
Coordinates   1112314..1113351 (+) Length   345 a.a.
NCBI ID   WP_006422551.1    Uniprot ID   -
Organism   Stenotrophomonas sp. NY11291     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1107314..1118351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1L61_RS05180 (M1L61_05180) soxR 1108565..1108999 (+) 435 WP_010483703.1 redox-sensitive transcriptional activator SoxR -
  M1L61_RS05185 (M1L61_05185) - 1109073..1109675 (-) 603 WP_049410246.1 short chain dehydrogenase -
  M1L61_RS05190 (M1L61_05190) - 1109774..1110649 (+) 876 WP_049481362.1 LysR family transcriptional regulator -
  M1L61_RS05195 (M1L61_05195) proC 1110709..1111530 (-) 822 WP_248854408.1 pyrroline-5-carboxylate reductase -
  M1L61_RS05200 (M1L61_05200) - 1111549..1112226 (-) 678 WP_100447906.1 YggS family pyridoxal phosphate-dependent enzyme -
  M1L61_RS05205 (M1L61_05205) pilT 1112314..1113351 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  M1L61_RS05210 (M1L61_05210) pilU 1113431..1114594 (+) 1164 WP_049434422.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M1L61_RS05215 (M1L61_05215) - 1114743..1115726 (+) 984 WP_043398309.1 aldo/keto reductase -
  M1L61_RS05220 (M1L61_05220) - 1115769..1116665 (-) 897 WP_043398312.1 LysR family transcriptional regulator -
  M1L61_RS05225 (M1L61_05225) - 1117046..1117258 (+) 213 WP_014646220.1 DUF4287 domain-containing protein -
  M1L61_RS05230 (M1L61_05230) - 1117368..1118075 (+) 708 WP_029379885.1 YitT family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38644.38 Da        Isoelectric Point: 6.6652

>NTDB_id=685828 M1L61_RS05205 WP_006422551.1 1112314..1113351(+) (pilT) [Stenotrophomonas sp. NY11291]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQARDYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=685828 M1L61_RS05205 WP_006422551.1 1112314..1113351(+) (pilT) [Stenotrophomonas sp. NY11291]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTTCGCCGGATCAACATCCCAGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATTCCGTCGCTGGCGCGC
TTCCGTGTGAACGCGTTCAACCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTCGA
GGACCTGGCCTGCCCGCCGTTGTTCCGCGAAGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGTGATACGCATGGTTTCAATGA
AGCGCTGCGCTCGGCACTGCGTGAGGACCCGGACATCATCCTGGTCGGCGAACTTCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGTGCCGTGATCTCGCAGGC
GCTGCTGAAGAAGGTTGGTGGCGGTCGTACCGCTGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGACTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391