Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M1L61_RS05210 Genome accession   NZ_CP096975
Coordinates   1113431..1114594 (+) Length   387 a.a.
NCBI ID   WP_049434422.1    Uniprot ID   A0AA43BPT5
Organism   Stenotrophomonas sp. NY11291     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1108431..1119594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1L61_RS05180 (M1L61_05180) soxR 1108565..1108999 (+) 435 WP_010483703.1 redox-sensitive transcriptional activator SoxR -
  M1L61_RS05185 (M1L61_05185) - 1109073..1109675 (-) 603 WP_049410246.1 short chain dehydrogenase -
  M1L61_RS05190 (M1L61_05190) - 1109774..1110649 (+) 876 WP_049481362.1 LysR family transcriptional regulator -
  M1L61_RS05195 (M1L61_05195) proC 1110709..1111530 (-) 822 WP_248854408.1 pyrroline-5-carboxylate reductase -
  M1L61_RS05200 (M1L61_05200) - 1111549..1112226 (-) 678 WP_100447906.1 YggS family pyridoxal phosphate-dependent enzyme -
  M1L61_RS05205 (M1L61_05205) pilT 1112314..1113351 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  M1L61_RS05210 (M1L61_05210) pilU 1113431..1114594 (+) 1164 WP_049434422.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M1L61_RS05215 (M1L61_05215) - 1114743..1115726 (+) 984 WP_043398309.1 aldo/keto reductase -
  M1L61_RS05220 (M1L61_05220) - 1115769..1116665 (-) 897 WP_043398312.1 LysR family transcriptional regulator -
  M1L61_RS05225 (M1L61_05225) - 1117046..1117258 (+) 213 WP_014646220.1 DUF4287 domain-containing protein -
  M1L61_RS05230 (M1L61_05230) - 1117368..1118075 (+) 708 WP_029379885.1 YitT family protein -
  M1L61_RS05235 (M1L61_05235) - 1118162..1118707 (-) 546 WP_164191945.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43186.63 Da        Isoelectric Point: 6.6615

>NTDB_id=685829 M1L61_RS05210 WP_049434422.1 1113431..1114594(+) (pilU) [Stenotrophomonas sp. NY11291]
MPHWEPAVNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNQNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVM
KDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=685829 M1L61_RS05210 WP_049434422.1 1113431..1114594(+) (pilU) [Stenotrophomonas sp. NY11291]
ATGCCCCATTGGGAGCCCGCCGTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCA
GCGCGCCTCGGACCTGTTCATCACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGA
CTCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACC
CACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGTCGTTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTGGG
CATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAGTTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCCACCGGCACCGGTAAGTCAACGTCGCTGGCGGCGATGATCGGTTAC
CGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCAT
CACCCAGCGTGAAGTCGGCATCGATACCGACAGCTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGA
TCATGATCGGCGAAGTGCGTACCCGCGAAGGCATGGACCATGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGC
ACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCT
GATGGACCTGTCGCTGAATCTCAAAGGCGTGGTCGCTCAGCAGCTGATTCCTTCGCCCGATGGCCGCTCGCGCAAGGTGG
CGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGATGGCGAGATTCACAAGCTGAAGGAAGTGATG
AAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAGATCAGCTACGA
GGACGCACTGCGCTACGCCGATTCGCAGAACGAGGTACGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGCACCC
TGTCGCAGGGGCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

94.832

0.641
  pilU Acinetobacter baylyi ADP1

63.866

92.248

0.589
  pilU Vibrio cholerae strain A1552

53.371

91.99

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

91.731

0.395