Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M2J84_RS17450 Genome accession   NZ_CP096918
Coordinates   3708729..3709772 (+) Length   347 a.a.
NCBI ID   WP_043383872.1    Uniprot ID   A0A014P1E9
Organism   Comamonas aquatica strain NY8661     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3703729..3714772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J84_RS17430 (M2J84_17445) - 3703788..3706001 (+) 2214 WP_416232841.1 TonB-dependent receptor domain-containing protein -
  M2J84_RS17435 (M2J84_17450) - 3706168..3706572 (-) 405 WP_270149744.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  M2J84_RS17440 (M2J84_17455) - 3706598..3707749 (-) 1152 WP_270149751.1 aminotransferase class V-fold PLP-dependent enzyme -
  M2J84_RS17445 (M2J84_17460) - 3707920..3708639 (-) 720 WP_270149753.1 YggS family pyridoxal phosphate-dependent enzyme -
  M2J84_RS17450 (M2J84_17465) pilT 3708729..3709772 (+) 1044 WP_043383872.1 type IV pilus twitching motility protein PilT Machinery gene
  M2J84_RS17455 (M2J84_17470) pilU 3709797..3710933 (+) 1137 WP_270149756.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M2J84_RS17460 (M2J84_17475) - 3710997..3711656 (-) 660 WP_270149758.1 BON domain-containing protein -
  M2J84_RS17465 (M2J84_17480) - 3711656..3712252 (-) 597 WP_042417407.1 SIS domain-containing protein -
  M2J84_RS17470 (M2J84_17485) - 3712367..3712765 (-) 399 WP_270149760.1 YraN family protein -
  M2J84_RS17475 (M2J84_17490) rsmI 3712765..3713688 (+) 924 WP_270149762.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37942.59 Da        Isoelectric Point: 7.1069

>NTDB_id=685157 M2J84_RS17450 WP_043383872.1 3708729..3709772(+) (pilT) [Comamonas aquatica strain NY8661]
MDITTLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEPLDHKQVHAMVYDIMNDGQRKAYEENLEVDFSFEIAGLAR
FRVNAFNQYRGAAAVFRTIPSKVLTLEELNAPKILADLALKPRGLVLVTGPTGSGKSTTLAAMVDHLNNSEHGHILTVED
PIEFVHNSKKCLVNQREVGPMTHSFAAALRSALREDPDCILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGNGRVAAHEIMLGTSAIRNLIREAKVAQMYSAIQTGSNVGMQTLDQN
LTELVRRNVISAAEARGKAKSPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=685157 M2J84_RS17450 WP_043383872.1 3708729..3709772(+) (pilT) [Comamonas aquatica strain NY8661]
GTGGACATCACCACCCTGCTGGCTTTCAGCGTGAAGAACAAGGCGTCGGACCTGCACCTGTCCGCCGGGTTGCCGCCCAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAATGTCGAGCCGCTGGACCACAAGCAGGTGCACGCCATGGTCTACGACA
TCATGAACGACGGCCAGCGCAAGGCCTACGAAGAAAACCTGGAGGTGGACTTCTCGTTCGAGATCGCCGGGCTGGCGCGT
TTCCGTGTCAACGCCTTCAACCAGTACCGCGGTGCGGCCGCCGTGTTCCGCACGATTCCCAGCAAGGTGCTGACACTGGA
AGAGCTGAACGCCCCCAAGATTTTGGCCGACCTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCCATGGTCGACCACCTCAACAACAGCGAGCACGGCCACATCCTGACGGTGGAAGAC
CCGATCGAATTCGTGCACAACTCCAAGAAATGCCTGGTCAACCAGCGCGAAGTCGGGCCGATGACGCACTCCTTTGCCGC
GGCCCTGCGTTCGGCGCTGCGCGAAGACCCGGACTGCATCCTGGTGGGTGAAATGCGCGACCTGGAAACCATCCGCCTGG
CGATGACGGCCGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGTATC
ATCGACGTGTTTCCGGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAGTCCCTGCAGGCGGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCAACGGCCGGGTGGCAGCCCACGAAATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGGCCCAGATGTATTCCGCCATCCAGACCGGCAGCAATGTCGGCATGCAGACCCTGGACCAGAAC
CTGACCGAGCTGGTGCGCCGCAACGTCATCAGCGCCGCCGAGGCGCGCGGCAAGGCCAAATCGCCGGACAACTTCCCCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A014P1E9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

75.529

95.389

0.72
  pilT Legionella pneumophila strain ERS1305867

75.529

95.389

0.72
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.456

97.406

0.657
  pilT Vibrio cholerae strain A1552

67.456

97.406

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.424

0.516
  pilU Vibrio cholerae strain A1552

42.319

99.424

0.421
  pilU Pseudomonas stutzeri DSM 10701

41.449

99.424

0.412
  pilU Acinetobacter baylyi ADP1

40.173

99.712

0.401