Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M2J84_RS17455 Genome accession   NZ_CP096918
Coordinates   3709797..3710933 (+) Length   378 a.a.
NCBI ID   WP_270149756.1    Uniprot ID   -
Organism   Comamonas aquatica strain NY8661     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3704797..3715933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J84_RS17435 (M2J84_17450) - 3706168..3706572 (-) 405 WP_270149744.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  M2J84_RS17440 (M2J84_17455) - 3706598..3707749 (-) 1152 WP_270149751.1 aminotransferase class V-fold PLP-dependent enzyme -
  M2J84_RS17445 (M2J84_17460) - 3707920..3708639 (-) 720 WP_270149753.1 YggS family pyridoxal phosphate-dependent enzyme -
  M2J84_RS17450 (M2J84_17465) pilT 3708729..3709772 (+) 1044 WP_043383872.1 type IV pilus twitching motility protein PilT Machinery gene
  M2J84_RS17455 (M2J84_17470) pilU 3709797..3710933 (+) 1137 WP_270149756.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M2J84_RS17460 (M2J84_17475) - 3710997..3711656 (-) 660 WP_270149758.1 BON domain-containing protein -
  M2J84_RS17465 (M2J84_17480) - 3711656..3712252 (-) 597 WP_042417407.1 SIS domain-containing protein -
  M2J84_RS17470 (M2J84_17485) - 3712367..3712765 (-) 399 WP_270149760.1 YraN family protein -
  M2J84_RS17475 (M2J84_17490) rsmI 3712765..3713688 (+) 924 WP_270149762.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  M2J84_RS17480 (M2J84_17495) - 3713798..3715048 (-) 1251 WP_270149764.1 Hsp70 family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42210.78 Da        Isoelectric Point: 6.4340

>NTDB_id=685158 M2J84_RS17455 WP_270149756.1 3709797..3710933(+) (pilU) [Comamonas aquatica strain NY8661]
MERDQASKFINDLLKLMVARKGSDLFITAEFPPAIKVDGKVTRLMEQPLVPTHTLMLARSIMSDKQVAEFERTKECNFAI
SPAGIGRFRVNAFIQQGRVGMVLRTIPADIPTIDGMGLPQVLKEVAMTKRGLCIFVGATGSGKSTSLAAMVDWRNEHSEG
HIVTVEDPIEFVHPHKNCIITQREVGLDTDSWEAALKNSLRQAPDVILMGEIRDRETMEHAIVFSETGHLCLATLHANSA
NQALDRIVNFFPEERREQLLMDLSLNLRAMISQRLLPKEGGQGRVAALEVMLNTPLISDLIAKGDVVEIKEIMKKSRELG
MQTFDQSLFDLYESGAVMYEDALRNADSVNDLRLQIKLHSKRAHNTDLAAGTEHFRIL

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=685158 M2J84_RS17455 WP_270149756.1 3709797..3710933(+) (pilU) [Comamonas aquatica strain NY8661]
ATGGAACGCGATCAGGCCAGCAAATTCATCAACGACCTTCTCAAGCTGATGGTGGCGCGCAAGGGCAGCGACCTGTTCAT
CACCGCCGAATTTCCGCCCGCCATCAAGGTCGACGGCAAGGTCACACGGCTGATGGAGCAGCCGCTGGTGCCCACGCACA
CCCTGATGCTGGCACGCTCCATCATGAGCGACAAGCAAGTGGCGGAGTTCGAACGCACCAAGGAGTGCAACTTCGCCATC
TCACCCGCCGGCATCGGGCGTTTTCGCGTCAACGCCTTCATCCAGCAGGGCCGCGTGGGTATGGTGCTGCGGACCATTCC
GGCCGACATCCCCACCATCGACGGCATGGGCCTGCCCCAGGTGCTGAAGGAAGTGGCCATGACCAAGCGCGGCCTGTGCA
TCTTTGTGGGGGCCACCGGGTCGGGCAAGTCGACCTCGCTGGCGGCCATGGTGGACTGGCGCAACGAACACTCCGAGGGG
CACATCGTCACGGTGGAAGACCCCATCGAATTCGTGCACCCCCACAAGAACTGCATCATCACCCAGCGCGAAGTGGGTCT
GGACACCGACAGTTGGGAAGCGGCCCTCAAGAACTCGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCCATCGTGTTCTCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGTGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCGGAAGAGCGCCGCGAGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCGCCATGATCTCGCAGCGCCTGCTGCCCAAAGAAGGCGGCCAGGGCCGTGTGGCCGCGCTGGAGGTGATGCTCAACA
CCCCACTGATCTCGGACCTGATCGCCAAGGGCGACGTGGTGGAAATCAAGGAAATCATGAAGAAAAGCCGCGAGCTGGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTACGAAAGCGGCGCCGTCATGTACGAGGATGCGCTGCGCAACGCTGA
CTCGGTGAACGACCTGCGCCTGCAGATCAAGCTGCACAGCAAACGTGCCCACAACACCGACCTGGCGGCGGGTACCGAGC
ACTTCCGCATTCTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.219

95.503

0.585
  pilU Acinetobacter baylyi ADP1

59.143

92.593

0.548
  pilU Vibrio cholerae strain A1552

55.172

92.063

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Acinetobacter baylyi ADP1

44.512

86.772

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Legionella pneumophila strain ERS1305867

45.031

85.185

0.384
  pilT Legionella pneumophila strain Lp02

45.031

85.185

0.384
  pilT Vibrio cholerae strain A1552

43.598

86.772

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.598

86.772

0.378