Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M0C34_RS16510 Genome accession   NZ_CP096670
Coordinates   3638428..3639462 (-) Length   344 a.a.
NCBI ID   WP_248712770.1    Uniprot ID   -
Organism   Agarivorans sp. TSD2052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3633428..3644462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0C34_RS16490 (M0C34_16490) srmB 3634236..3635492 (+) 1257 WP_248712766.1 ATP-dependent RNA helicase SrmB -
  M0C34_RS16495 (M0C34_16495) - 3635566..3636297 (-) 732 WP_248712767.1 OmpA family protein -
  M0C34_RS16500 (M0C34_16500) yaaA 3636499..3637278 (-) 780 WP_248712768.1 peroxide stress protein YaaA -
  M0C34_RS16505 (M0C34_16505) pilU 3637301..3638419 (-) 1119 WP_248712769.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M0C34_RS16510 (M0C34_16510) pilT 3638428..3639462 (-) 1035 WP_248712770.1 type IV pilus twitching motility protein PilT Machinery gene
  M0C34_RS16515 (M0C34_16515) - 3639489..3640187 (+) 699 WP_248712771.1 YggS family pyridoxal phosphate-dependent enzyme -
  M0C34_RS16520 (M0C34_16520) proC 3640211..3641032 (+) 822 WP_248712772.1 pyrroline-5-carboxylate reductase -
  M0C34_RS16525 (M0C34_16525) - 3641050..3641595 (+) 546 WP_248712773.1 YggT family protein -
  M0C34_RS16530 (M0C34_16530) yggU 3641596..3641883 (+) 288 WP_248712774.1 DUF167 family protein YggU -
  M0C34_RS16535 (M0C34_16535) - 3641942..3642376 (+) 435 WP_248712775.1 DUF4426 domain-containing protein -
  M0C34_RS16540 (M0C34_16540) rdgB 3642483..3643082 (+) 600 WP_248712776.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  M0C34_RS16545 (M0C34_16545) hemW 3643076..3644230 (+) 1155 WP_248712777.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38410.00 Da        Isoelectric Point: 6.3899

>NTDB_id=682226 M0C34_RS16510 WP_248712770.1 3638428..3639462(-) (pilT) [Agarivorans sp. TSD2052]
MDVTELLAFSVKHNASDLHLSAELPPMIRVDGEVRKINLPALEHKQVHSLIYDIMNDKQRKEYEENLEIDFSFEVPSIAR
FRVNAFQQNRGAAAVFRTIPSEVLTLDQLGAPAIFRTISEHPRGLVLVTGPTGSGKSTTLAAMIDYINEQRHEHILTIED
PIEFVHKNKNCLINQREVHRDTHSFNNALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLRAVIAQTLLKKLGGGRVAAHEIMIGIPAIRNLIREDKVAQMYSVIQTGMAHGMQTLDQNL
KELVNQGMVSYEDAKTKAADPNNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=682226 M0C34_RS16510 WP_248712770.1 3638428..3639462(-) (pilT) [Agarivorans sp. TSD2052]
ATGGATGTTACCGAATTACTGGCCTTTAGTGTAAAACATAACGCGTCAGATCTACACCTATCAGCTGAATTACCGCCAAT
GATTCGAGTTGATGGTGAAGTTAGAAAAATAAACTTACCTGCGCTTGAACATAAACAAGTTCATAGTTTGATTTATGACA
TTATGAACGACAAACAGCGAAAAGAGTATGAAGAGAACTTAGAAATCGATTTCTCGTTTGAGGTTCCAAGTATTGCTCGC
TTCAGGGTTAACGCCTTCCAACAGAACCGTGGTGCAGCAGCGGTATTCAGGACGATTCCTTCAGAAGTATTGACCTTGGA
TCAGTTGGGCGCGCCTGCGATCTTTCGCACAATATCTGAGCATCCTCGTGGCTTGGTATTGGTGACAGGGCCTACGGGTT
CGGGTAAATCAACCACGCTAGCGGCAATGATCGATTACATTAACGAGCAGCGCCATGAGCATATTCTTACCATTGAAGAC
CCGATAGAATTTGTTCACAAAAACAAAAACTGCTTGATCAACCAACGGGAAGTTCACCGAGACACCCATAGCTTTAATAA
CGCATTGCGTTCGGCACTGCGTGAAGATCCCGATATTATTCTGGTGGGTGAATTACGTGACTTAGAAACCATTCGTTTAG
CACTAACCGCAGCTGAAACGGGTCACTTAGTATTTGGCACCTTGCACACCACCTCAGCAGCTAAAACCATTGACCGTGTT
ATCGATGTATTTCCAGCGGCAGAAAAAGACATGGTTCGTTCTATGTTGTCAGAGTCATTACGCGCAGTAATCGCCCAAAC
TTTACTTAAAAAGCTTGGCGGTGGGCGAGTGGCCGCACATGAAATAATGATTGGTATTCCGGCTATTCGTAACTTAATCC
GCGAAGATAAAGTGGCGCAAATGTACTCTGTCATTCAAACTGGTATGGCGCATGGTATGCAAACCCTTGATCAAAATCTT
AAAGAGTTGGTAAACCAAGGCATGGTGTCTTATGAAGACGCTAAAACTAAAGCCGCTGATCCCAACAATTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.814

100

0.808
  pilT Acinetobacter baumannii D1279779

80.814

100

0.808
  pilT Acinetobacter baumannii strain A118

80.814

100

0.808
  pilT Pseudomonas stutzeri DSM 10701

78.779

100

0.788
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Pseudomonas aeruginosa PAK

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

74.128

100

0.741
  pilT Legionella pneumophila strain ERS1305867

74.128

100

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.184

97.965

0.727
  pilT Vibrio cholerae strain A1552

74.184

97.965

0.727
  pilT Neisseria meningitidis 8013

67.742

99.128

0.672
  pilT Neisseria gonorrhoeae MS11

67.449

99.128

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.506
  pilU Pseudomonas stutzeri DSM 10701

40.87

100

0.41
  pilU Vibrio cholerae strain A1552

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404