Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M0C34_RS16505 Genome accession   NZ_CP096670
Coordinates   3637301..3638419 (-) Length   372 a.a.
NCBI ID   WP_248712769.1    Uniprot ID   -
Organism   Agarivorans sp. TSD2052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3632301..3643419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0C34_RS16485 (M0C34_16485) - 3633412..3634128 (-) 717 WP_248712765.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  M0C34_RS16490 (M0C34_16490) srmB 3634236..3635492 (+) 1257 WP_248712766.1 ATP-dependent RNA helicase SrmB -
  M0C34_RS16495 (M0C34_16495) - 3635566..3636297 (-) 732 WP_248712767.1 OmpA family protein -
  M0C34_RS16500 (M0C34_16500) yaaA 3636499..3637278 (-) 780 WP_248712768.1 peroxide stress protein YaaA -
  M0C34_RS16505 (M0C34_16505) pilU 3637301..3638419 (-) 1119 WP_248712769.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M0C34_RS16510 (M0C34_16510) pilT 3638428..3639462 (-) 1035 WP_248712770.1 type IV pilus twitching motility protein PilT Machinery gene
  M0C34_RS16515 (M0C34_16515) - 3639489..3640187 (+) 699 WP_248712771.1 YggS family pyridoxal phosphate-dependent enzyme -
  M0C34_RS16520 (M0C34_16520) proC 3640211..3641032 (+) 822 WP_248712772.1 pyrroline-5-carboxylate reductase -
  M0C34_RS16525 (M0C34_16525) - 3641050..3641595 (+) 546 WP_248712773.1 YggT family protein -
  M0C34_RS16530 (M0C34_16530) yggU 3641596..3641883 (+) 288 WP_248712774.1 DUF167 family protein YggU -
  M0C34_RS16535 (M0C34_16535) - 3641942..3642376 (+) 435 WP_248712775.1 DUF4426 domain-containing protein -
  M0C34_RS16540 (M0C34_16540) rdgB 3642483..3643082 (+) 600 WP_248712776.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41562.70 Da        Isoelectric Point: 6.5663

>NTDB_id=682225 M0C34_RS16505 WP_248712769.1 3637301..3638419(-) (pilU) [Agarivorans sp. TSD2052]
MLLDDLLSDMSERKASDLYLSVGVPPSAKVSGRLTPLNEQKLMPEHVMAMLATIRTPAQLEAFEHGREANFAIARKGLGR
YRVSAFYQREQPSMVIRRIETNIPSFEQLQLPPILKEVGLSKRGLILFVGATGAGKSTTQASMIDYRNHNSSGHILTIED
PVEFMHKHAGCIVNQREVGIDTPSFEEALKNSLRQAPDVILIGEIRTRETMEFALQFSETGHLCMATLHANNANQALDRI
MHLVPEERHRQLCFDLSFNLRAIVAQQLVPTRDGSSRRGVFEILLNTPLAADLIRKGEMHKLKELMTKSREQGMLTFDQS
LFELYESSVIGYAEAIAHADSPNDLRLLIKLHGTDKSNSLDSGMLDGVTVDF

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=682225 M0C34_RS16505 WP_248712769.1 3637301..3638419(-) (pilU) [Agarivorans sp. TSD2052]
ATGTTACTCGATGATCTATTAAGCGATATGTCAGAGCGCAAAGCATCAGATTTGTATCTTTCTGTGGGGGTCCCGCCGAG
TGCAAAAGTGAGTGGCCGCTTAACCCCGTTAAATGAACAAAAACTCATGCCTGAGCATGTGATGGCGATGTTGGCGACTA
TTCGTACTCCTGCTCAGCTTGAGGCTTTTGAACATGGTCGAGAGGCCAACTTTGCTATCGCGCGCAAAGGCTTAGGCCGC
TACCGGGTGAGTGCATTTTATCAACGTGAACAGCCAAGCATGGTGATTCGCCGTATTGAGACAAATATTCCCAGTTTTGA
GCAGTTGCAATTGCCGCCGATTCTTAAAGAAGTTGGCTTGTCTAAGCGTGGCTTAATTTTGTTTGTGGGGGCAACGGGGG
CAGGTAAGTCTACCACTCAGGCCAGTATGATTGATTATCGCAACCATAATTCTAGCGGCCATATTCTCACCATTGAAGAT
CCGGTAGAATTTATGCACAAACACGCTGGTTGTATTGTTAATCAGCGCGAAGTAGGCATCGATACCCCTTCGTTTGAAGA
GGCCTTAAAGAACTCACTGCGTCAGGCTCCTGATGTGATTCTAATTGGCGAAATTCGTACCCGCGAAACCATGGAGTTCG
CCTTACAGTTCTCTGAAACTGGTCACCTTTGTATGGCGACGCTGCACGCCAACAATGCTAACCAAGCTTTAGATAGGATC
ATGCACTTAGTACCAGAAGAGCGGCATCGCCAGTTGTGTTTCGATTTGTCGTTTAACTTGCGGGCAATTGTGGCGCAGCA
ATTAGTGCCAACAAGAGATGGCAGTAGTCGACGCGGCGTATTTGAAATTTTGCTTAATACCCCCTTAGCGGCTGACCTGA
TCCGTAAAGGTGAAATGCATAAACTCAAAGAGCTGATGACGAAGTCTCGTGAGCAAGGGATGTTAACTTTTGACCAAAGC
TTATTTGAGCTATATGAGTCCAGTGTGATCGGTTACGCCGAGGCGATTGCGCATGCTGATTCTCCTAACGATTTACGTCT
ATTGATTAAATTGCATGGCACCGATAAATCGAATAGTTTAGACAGTGGTATGTTAGACGGTGTTACCGTCGATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.823

94.355

0.583
  pilU Acinetobacter baylyi ADP1

60.227

94.624

0.57
  pilU Vibrio cholerae strain A1552

56.183

100

0.562
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.816

92.204

0.376