Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CPY99_RS15715 Genome accession   NZ_AP017620
Coordinates   3141416..3142396 (-) Length   326 a.a.
NCBI ID   WP_072693229.1    Uniprot ID   -
Organism   Escherichia coli strain MRY15-131     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3136416..3147396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPY99_RS15685 (MRY15131_c28800) yggI 3137081..3137578 (+) 498 Protein_2940 SprT family zinc-dependent metalloprotease -
  CPY99_RS15690 (MRY15131_c28810) endA 3137673..3138380 (+) 708 WP_023149797.1 deoxyribonuclease I -
  CPY99_RS15695 (MRY15131_c28820) rsmE 3138460..3139191 (+) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CPY99_RS15700 (MRY15131_c28830) gshB 3139204..3140154 (+) 951 WP_000593273.1 glutathione synthase -
  CPY99_RS15705 (MRY15131_c28840) yqgE 3140263..3140826 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  CPY99_RS15710 (MRY15131_c28850) ruvX 3140826..3141242 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  CPY99_RS15715 (MRY15131_c28860) pilT 3141416..3142396 (-) 981 WP_072693229.1 type IV pilus twitching motility protein PilT Machinery gene
  CPY99_RS15720 (MRY15131_c28870) yggS 3142414..3143118 (+) 705 WP_000997804.1 pyridoxal phosphate homeostasis protein -
  CPY99_RS15725 (MRY15131_c28880) yggT 3143136..3143702 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  CPY99_RS15730 (MRY15131_c28890) yggU 3143699..3143989 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  CPY99_RS15735 (MRY15131_c28900) rdgB 3143997..3144590 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  CPY99_RS15740 (MRY15131_c28910) hemW 3144583..3145719 (+) 1137 WP_000239953.1 radical SAM family heme chaperone HemW -
  CPY99_RS15745 (MRY15131_c28920) yggM 3145844..3146791 (-) 948 WP_072693227.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35975.11 Da        Isoelectric Point: 5.9975

>NTDB_id=68145 CPY99_RS15715 WP_072693229.1 3141416..3142396(-) (pilT) [Escherichia coli strain MRY15-131]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAGLSQKLEVDKQEGRVALFESLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=68145 CPY99_RS15715 WP_072693229.1 3141416..3142396(-) (pilT) [Escherichia coli strain MRY15-131]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGGGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATCACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilB Legionella pneumophila strain ERS1305867

31.481

100

0.365
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment