Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CPZ02_RS16575 Genome accession   NZ_AP017610
Coordinates   3216991..3217971 (-) Length   326 a.a.
NCBI ID   WP_001696140.1    Uniprot ID   A0A1V2FQ82
Organism   Escherichia coli strain 20Ec-P-124     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3211991..3222971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPZ02_RS16545 (MRY16002_c29680) yggI 3212656..3213153 (+) 498 WP_001696137.1 SprT family zinc-dependent metalloprotease -
  CPZ02_RS16550 (MRY16002_c29690) endA 3213248..3213955 (+) 708 WP_001305312.1 deoxyribonuclease I -
  CPZ02_RS16555 (MRY16002_c29700) rsmE 3214035..3214766 (+) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CPZ02_RS16560 (MRY16002_c29710) gshB 3214779..3215729 (+) 951 WP_000593258.1 glutathione synthase -
  CPZ02_RS16565 (MRY16002_c29720) yqgE 3215838..3216401 (+) 564 WP_001318029.1 YqgE/AlgH family protein -
  CPZ02_RS16570 (MRY16002_c29730) ruvX 3216401..3216817 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  CPZ02_RS16575 (MRY16002_c29740) pilT 3216991..3217971 (-) 981 WP_001696140.1 type IV pilus twitching motility protein PilT Machinery gene
  CPZ02_RS16580 (MRY16002_c29750) yggS 3217989..3218693 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  CPZ02_RS16585 (MRY16002_c29760) yggT 3218711..3219277 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  CPZ02_RS16590 (MRY16002_c29770) yggU 3219274..3219564 (+) 291 WP_001277194.1 DUF167 family protein YggU -
  CPZ02_RS16595 (MRY16002_c29780) rdgB 3219572..3220165 (+) 594 WP_001174747.1 XTP/dITP diphosphatase -
  CPZ02_RS16600 (MRY16002_c29790) hemW 3220158..3221294 (+) 1137 WP_000239950.1 radical SAM family heme chaperone HemW -
  CPZ02_RS16605 (MRY16002_c29800) - 3221610..3222596 (+) 987 WP_000784004.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35987.21 Da        Isoelectric Point: 5.9975

>NTDB_id=68101 CPZ02_RS16575 WP_001696140.1 3216991..3217971(-) (pilT) [Escherichia coli strain 20Ec-P-124]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPAVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=68101 CPZ02_RS16575 WP_001696140.1 3216991..3217971(-) (pilT) [Escherichia coli strain 20Ec-P-124]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAGCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATGCAAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTAGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGTTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V2FQ82

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment