Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MTO69_RS11820 Genome accession   NZ_CP096199
Coordinates   2621113..2622150 (-) Length   345 a.a.
NCBI ID   WP_248329479.1    Uniprot ID   -
Organism   Vibrio sinaloensis strain YA2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2616113..2627150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTO69_RS11790 (MTO69_11790) - 2616505..2617002 (+) 498 WP_248329470.1 SprT family zinc-dependent metalloprotease -
  MTO69_RS11795 (MTO69_11795) rsmE 2617081..2617812 (+) 732 WP_248329472.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MTO69_RS11800 (MTO69_11800) gshB 2617826..2618773 (+) 948 WP_248329474.1 glutathione synthase -
  MTO69_RS11805 (MTO69_11805) - 2618826..2619389 (+) 564 WP_248329476.1 YqgE/AlgH family protein -
  MTO69_RS11810 (MTO69_11810) ruvX 2619407..2619829 (+) 423 WP_248329477.1 Holliday junction resolvase RuvX -
  MTO69_RS11815 (MTO69_11815) pilU 2619997..2621103 (-) 1107 WP_248329478.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MTO69_RS11820 (MTO69_11820) pilT 2621113..2622150 (-) 1038 WP_248329479.1 type IV pilus twitching motility protein PilT Machinery gene
  MTO69_RS11825 (MTO69_11825) - 2622176..2622892 (+) 717 WP_248329480.1 YggS family pyridoxal phosphate-dependent enzyme -
  MTO69_RS11830 (MTO69_11830) proC 2622923..2623741 (+) 819 WP_248329481.1 pyrroline-5-carboxylate reductase -
  MTO69_RS11835 (MTO69_11835) - 2623781..2624338 (+) 558 WP_248329482.1 YggT family protein -
  MTO69_RS11840 (MTO69_11840) yggU 2624338..2624628 (+) 291 WP_248329483.1 DUF167 family protein YggU -
  MTO69_RS11845 (MTO69_11845) - 2624665..2625096 (+) 432 WP_248329484.1 DUF4426 domain-containing protein -
  MTO69_RS11850 (MTO69_11850) - 2625164..2625766 (+) 603 WP_248329485.1 XTP/dITP diphosphatase -
  MTO69_RS11855 (MTO69_11855) hemW 2625766..2626941 (+) 1176 WP_248329486.1 radical SAM family heme chaperone HemW -
  MTO69_RS11860 (MTO69_11860) - 2626916..2627101 (+) 186 WP_248329488.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38071.66 Da        Isoelectric Point: 6.2693

>NTDB_id=680266 MTO69_RS11820 WP_248329479.1 2621113..2622150(-) (pilT) [Vibrio sinaloensis strain YA2]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFSHADVHRLVFDIMNDAQRSEFEERLEVDFSFELPDVGR
FRVNAFNQSRGCAAVFRTIPTQIPTLEQLATPAIFESIANMEKGLVLVTGPTGSGKSTTLAAMVDHINRQHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKEMVRSMLSESLKAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMVSVIQTGAAHGMQTMEQNA
KQLIAQGLVEQAEVAKKIELETPMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=680266 MTO69_RS11820 WP_248329479.1 2621113..2622150(-) (pilT) [Vibrio sinaloensis strain YA2]
ATGGATATCGCTGAGTTACTGGATTTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCTGGAGTATCTCCCAT
GGTGCGGATTGATGGTGAAGTTCGCAAGTTGGGCGTGCCCGCTTTTAGTCACGCGGATGTACATCGCTTGGTGTTCGATA
TTATGAACGACGCTCAGCGCAGCGAGTTTGAAGAAAGACTCGAGGTCGACTTTTCTTTTGAGTTGCCCGATGTCGGACGC
TTTCGTGTCAACGCTTTCAACCAGTCTCGCGGCTGTGCGGCAGTCTTTCGTACCATTCCCACCCAGATCCCAACCCTCGA
ACAGCTTGCAACACCGGCTATCTTCGAGTCGATTGCTAATATGGAAAAGGGCTTGGTCTTAGTGACCGGTCCAACTGGCT
CAGGTAAGTCGACCACCTTAGCGGCGATGGTTGACCATATTAATCGCCAGCACAATAAGCATATCCTCACCATTGAAGAT
CCGATTGAGTTTGTCCATAGCAACAATAAGTGTTTGATTAACCAACGCGAGGTCCATCGCGATACCCATAGCTTCAATAA
TGCTCTGCGTAGTGCCTTGCGTGAAGATCCGGATGTGATTTTGGTCGGCGAGTTGCGTGATCAAGAGACCATCAGCTTAG
CCTTAACCGCCGCAGAAACCGGCCATTTGGTGTTTGGCACCTTACACACTAGCTCAGCGGCAAAAACCATTGACCGAATT
ATTGATGTTTTCCCGGGCAGCGATAAAGAGATGGTGCGTTCAATGTTATCGGAGTCACTCAAAGCGGTGATTGCACAAAA
ACTCCTAAAACGCATTGGCGGTGGGCGCGTGGCTTGTCACGAGATTATGATGGCGACCCCAGCGATTCGAAACTTAATCC
GTGAAGACAAGGTGGCGCAAATGGTGTCGGTGATTCAAACCGGTGCGGCTCATGGGATGCAGACGATGGAGCAAAATGCC
AAACAGTTGATTGCCCAAGGCTTGGTTGAACAAGCCGAAGTGGCGAAGAAAATCGAACTCGAAACCCCGATGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.826

100

0.878
  pilT Vibrio cholerae strain A1552

87.826

100

0.878
  pilT Acinetobacter baumannii D1279779

71.733

95.362

0.684
  pilT Acinetobacter baumannii strain A118

71.733

95.362

0.684
  pilT Acinetobacter nosocomialis M2

71.429

95.362

0.681
  pilT Acinetobacter baylyi ADP1

71.125

95.362

0.678
  pilT Pseudomonas stutzeri DSM 10701

68.249

97.681

0.667
  pilT Pseudomonas aeruginosa PAK

67.059

98.551

0.661
  pilT Legionella pneumophila strain Lp02

67.674

95.942

0.649
  pilT Legionella pneumophila strain ERS1305867

67.674

95.942

0.649
  pilT Neisseria meningitidis 8013

65.089

97.971

0.638
  pilT Neisseria gonorrhoeae MS11

65.455

95.652

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.727

95.652

0.504
  pilU Vibrio cholerae strain A1552

43.692

94.203

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.463

95.072

0.394
  pilU Acinetobacter baylyi ADP1

40.938

92.754

0.38