Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MTO69_RS11815 Genome accession   NZ_CP096199
Coordinates   2619997..2621103 (-) Length   368 a.a.
NCBI ID   WP_248329478.1    Uniprot ID   -
Organism   Vibrio sinaloensis strain YA2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2614997..2626103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTO69_RS11785 (MTO69_11785) - 2615637..2616434 (+) 798 WP_248329468.1 DUF2189 domain-containing protein -
  MTO69_RS11790 (MTO69_11790) - 2616505..2617002 (+) 498 WP_248329470.1 SprT family zinc-dependent metalloprotease -
  MTO69_RS11795 (MTO69_11795) rsmE 2617081..2617812 (+) 732 WP_248329472.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MTO69_RS11800 (MTO69_11800) gshB 2617826..2618773 (+) 948 WP_248329474.1 glutathione synthase -
  MTO69_RS11805 (MTO69_11805) - 2618826..2619389 (+) 564 WP_248329476.1 YqgE/AlgH family protein -
  MTO69_RS11810 (MTO69_11810) ruvX 2619407..2619829 (+) 423 WP_248329477.1 Holliday junction resolvase RuvX -
  MTO69_RS11815 (MTO69_11815) pilU 2619997..2621103 (-) 1107 WP_248329478.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MTO69_RS11820 (MTO69_11820) pilT 2621113..2622150 (-) 1038 WP_248329479.1 type IV pilus twitching motility protein PilT Machinery gene
  MTO69_RS11825 (MTO69_11825) - 2622176..2622892 (+) 717 WP_248329480.1 YggS family pyridoxal phosphate-dependent enzyme -
  MTO69_RS11830 (MTO69_11830) proC 2622923..2623741 (+) 819 WP_248329481.1 pyrroline-5-carboxylate reductase -
  MTO69_RS11835 (MTO69_11835) - 2623781..2624338 (+) 558 WP_248329482.1 YggT family protein -
  MTO69_RS11840 (MTO69_11840) yggU 2624338..2624628 (+) 291 WP_248329483.1 DUF167 family protein YggU -
  MTO69_RS11845 (MTO69_11845) - 2624665..2625096 (+) 432 WP_248329484.1 DUF4426 domain-containing protein -
  MTO69_RS11850 (MTO69_11850) - 2625164..2625766 (+) 603 WP_248329485.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41304.02 Da        Isoelectric Point: 6.4516

>NTDB_id=680265 MTO69_RS11815 WP_248329478.1 2619997..2621103(-) (pilU) [Vibrio sinaloensis strain YA2]
MDSDRYLQQMIEQKSSDLYITVGAPILYRVDGELKAQGETLSHAMVTSLIEGMMDTERQHEFHRSKEANFAIVRHFGRFR
VSAFYQREQPGAVIRRIETSIPSFEQLKLPEVLKQIALAKRGLVLVVGATGSGKSTTMAAMTGYRNQNSSGHILTVEDPI
EFVHEHQGCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGIIAQQLVRDKHGQGRHGAFEVLLNSPRVSDLIRRGDLHELKTTMAKSNQAGMQTFDQALY
QLVIEDKISEEDALHSADSANDLRILLKSHSGARTSLSSLADVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=680265 MTO69_RS11815 WP_248329478.1 2619997..2621103(-) (pilU) [Vibrio sinaloensis strain YA2]
ATGGATAGTGATCGTTACTTGCAACAAATGATCGAACAGAAGAGTTCGGATCTGTATATCACGGTAGGCGCACCGATTTT
ATATCGCGTTGACGGGGAGCTGAAAGCGCAGGGCGAGACGCTCTCTCATGCGATGGTTACCTCTTTAATCGAAGGCATGA
TGGACACTGAGCGCCAACATGAGTTTCATCGCAGCAAAGAGGCGAATTTTGCCATTGTTCGCCACTTTGGCCGTTTTCGG
GTGTCGGCTTTTTATCAGCGCGAGCAGCCTGGTGCGGTGATTCGTCGTATCGAAACCAGTATTCCTAGTTTTGAGCAGCT
TAAACTGCCGGAAGTGTTAAAACAGATCGCCTTGGCTAAACGTGGTTTGGTGCTGGTGGTTGGAGCAACAGGATCAGGAA
AATCGACCACCATGGCCGCGATGACCGGCTATCGTAATCAAAACAGCAGCGGTCATATCCTGACCGTAGAAGACCCGATT
GAGTTTGTTCATGAGCATCAAGGCTGCATAGTGACTCAGCGCGAAGTGGGATTGGACACAGAAAGCTACGAAGTGGCGTT
GAAAAACTCGCTACGCCAAGCTCCGGATATGATTTTGATCGGTGAAATCCGCTCGCGAGAAACCATGGAGTACGCGATGA
CCTTCGCCGAAACCGGCCATCTATGCATGGCGACACTGCATGCAAACAATGCCAACCAAGCATTAGAGCGTATCTTGCAC
TTGGTGCCCAAAGAGCAAAAAGAACAGTTCTTGTTTGATTTGTCGATGAACCTCAGAGGTATCATCGCCCAGCAGTTAGT
GCGCGATAAACATGGTCAGGGGCGTCATGGTGCGTTCGAGGTGCTTTTGAATAGTCCGCGCGTGTCTGATTTGATTCGCC
GCGGGGATTTACATGAGCTGAAAACCACGATGGCGAAGTCAAATCAGGCGGGGATGCAGACTTTTGATCAAGCCCTTTAT
CAGTTGGTGATTGAAGACAAAATCAGCGAAGAAGATGCGTTGCATAGCGCCGATTCGGCCAACGATCTGCGGATACTGCT
CAAATCGCACTCAGGCGCTCGAACCAGCCTAAGCAGTCTCGCAGATGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

79.348

100

0.793
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

53.039

98.37

0.522
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

39.883

92.663

0.37
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37
  pilT Acinetobacter baumannii D1279779

39.823

92.12

0.367
  pilT Acinetobacter baumannii strain A118

39.823

92.12

0.367
  pilT Acinetobacter nosocomialis M2

39.823

92.12

0.367
  pilT Vibrio cholerae strain A1552

41.538

88.315

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.538

88.315

0.367