Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MZ182_RS14085 Genome accession   NZ_CP096169
Coordinates   3151367..3152404 (-) Length   345 a.a.
NCBI ID   WP_011788674.1    Uniprot ID   A0A6N3J320
Organism   Shewanella sp. JNE2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3146367..3157404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MZ182_RS14050 (MZ182_14050) - 3146510..3146824 (+) 315 WP_011919670.1 hypothetical protein -
  MZ182_RS14055 (MZ182_14055) yciH 3146990..3147319 (+) 330 WP_011788680.1 stress response translation initiation inhibitor YciH -
  MZ182_RS14060 (MZ182_14060) - 3147432..3147995 (+) 564 WP_011788679.1 YqgE/AlgH family protein -
  MZ182_RS14065 (MZ182_14065) ruvX 3148027..3148452 (+) 426 WP_011788678.1 Holliday junction resolvase RuvX -
  MZ182_RS14070 (MZ182_14070) hemH 3148521..3149534 (-) 1014 WP_248065814.1 ferrochelatase -
  MZ182_RS14075 (MZ182_14075) - 3149567..3150106 (-) 540 WP_248065813.1 glutathione peroxidase -
  MZ182_RS14080 (MZ182_14080) pilU 3150245..3151357 (-) 1113 WP_248065812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MZ182_RS14085 (MZ182_14085) pilT 3151367..3152404 (-) 1038 WP_011788674.1 type IV pilus twitching motility protein PilT Machinery gene
  MZ182_RS14090 (MZ182_14090) - 3152440..3153138 (+) 699 WP_011788673.1 YggS family pyridoxal phosphate-dependent enzyme -
  MZ182_RS14095 (MZ182_14095) proC 3153319..3154137 (+) 819 WP_248065811.1 pyrroline-5-carboxylate reductase -
  MZ182_RS14100 (MZ182_14100) - 3154184..3154732 (+) 549 WP_011788671.1 YggT family protein -
  MZ182_RS14105 (MZ182_14105) yggU 3154732..3155022 (+) 291 WP_011788670.1 DUF167 family protein YggU -
  MZ182_RS14110 (MZ182_14110) - 3155100..3155534 (+) 435 WP_011788669.1 DUF4426 domain-containing protein -
  MZ182_RS14115 (MZ182_14115) rdgB 3155645..3156262 (+) 618 WP_011919679.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38339.04 Da        Isoelectric Point: 7.0500

>NTDB_id=680193 MZ182_RS14085 WP_011788674.1 3151367..3152404(-) (pilT) [Shewanella sp. JNE2]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHRGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASYPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHPNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMSKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=680193 MZ182_RS14085 WP_011788674.1 3151367..3152404(-) (pilT) [Shewanella sp. JNE2]
ATGGAAATCACTGAGTTATTAGCTTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTTGACGGTGAAGTGAGAAAAATTAACCTACCAGCGCTCGATCATCGGGGCGTACACAGCTTAGTGTACGATA
TTATGAATGATAAACAGCGCAAGGACTTTGAAGAGCATTTAGAAATTGATTTTTCGTTTGAAGTGCCCAACTTAGCCCGT
TTTCGTGTAAACGCATTTAACCAATCCCGTGGTGCCGCCGCAGTATTTCGTACCATTCCCAGTGATATATTGTCACTTGA
ACAACTTGGCGCACCGGAGATATTCAAAAAAATTGCCAGTTATCCGCGTGGTTTAGTGTTAGTCACTGGACCGACTGGCT
CGGGTAAGAGTACCACACTTGCAGCAATGGTGGATTACATCAATGAAAACCGCCATGATCATATCTTAACCATTGAAGAC
CCAATTGAATTTGTCCATCCTAATAAACAATGTTTGATTAACCAGAGGGAAGTTCATCGGCATACACACAGTTTTAACGC
TGCCCTCAGGAGCGCCTTGCGCGAGGATCCAGACGTAATTTTAGTGGGTGAGATGCGTGATCTTGAAACCATACGCCTAG
CGATGACCGCCGCAGAAACTGGCCACTTAGTCTTCGGTACTTTACACACAACTTCAGCAGCGAAGACGATTGACCGTGTG
GTGGATGTATTTCCTGCTGGGGAGAAAGACATGGTACGCACTATGTTATCTGAATCATTACAAGCGGTTATTTCACAGAC
GCTTATCAAAAAAATTGGCGGTGGCCGTGTCGCTGCACACGAAATTATGATGGGAACACCTGCAATTCGTAACTTGATCC
GTGAGGACAAAGTCGCACAAATGTACTCTGCCATTCAAACTGGCATGGCTCATGGAATGCAGACTCTGGAGCAATGCCTG
CAAAATTTAGTTAATCGTGGCCTGATCACCCGCGAAGACGCCATGTCGAAAAGCTCAAATAAACAAGCCACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N3J320

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.592

99.42

0.791
  pilT Acinetobacter baumannii strain A118

79.592

99.42

0.791
  pilT Acinetobacter nosocomialis M2

79.592

99.42

0.791
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baylyi ADP1

80.851

95.362

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

74.487

98.841

0.736
  pilT Legionella pneumophila strain ERS1305867

74.487

98.841

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.997

97.681

0.713
  pilT Vibrio cholerae strain A1552

72.997

97.681

0.713
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.691

99.42

0.414
  pilU Vibrio cholerae strain A1552

41.667

97.391

0.406
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391