NTDB

Overview

Name	pilU	Type	Machinery gene
Locus tag	MZ182_RS14080	Genome accession	NZ_CP096169
Coordinates	3150245..3151357 (-)	Length	370 a.a.
NCBI ID	WP_248065812.1	Uniprot ID	-
Organism	Shewanella sp. JNE2
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3145245..3156357

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MZ182_RS14045 (MZ182_14045)	-	3145771..3146331 (-)	561	WP_011788682.1	DUF4136 domain-containing protein	-
MZ182_RS14050 (MZ182_14050)	-	3146510..3146824 (+)	315	WP_011919670.1	hypothetical protein	-
MZ182_RS14055 (MZ182_14055)	yciH	3146990..3147319 (+)	330	WP_011788680.1	stress response translation initiation inhibitor YciH	-
MZ182_RS14060 (MZ182_14060)	-	3147432..3147995 (+)	564	WP_011788679.1	YqgE/AlgH family protein	-
MZ182_RS14065 (MZ182_14065)	ruvX	3148027..3148452 (+)	426	WP_011788678.1	Holliday junction resolvase RuvX	-
MZ182_RS14070 (MZ182_14070)	hemH	3148521..3149534 (-)	1014	WP_248065814.1	ferrochelatase	-
MZ182_RS14075 (MZ182_14075)	-	3149567..3150106 (-)	540	WP_248065813.1	glutathione peroxidase	-
MZ182_RS14080 (MZ182_14080)	pilU	3150245..3151357 (-)	1113	WP_248065812.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MZ182_RS14085 (MZ182_14085)	pilT	3151367..3152404 (-)	1038	WP_011788674.1	type IV pilus twitching motility protein PilT	Machinery gene
MZ182_RS14090 (MZ182_14090)	-	3152440..3153138 (+)	699	WP_011788673.1	YggS family pyridoxal phosphate-dependent enzyme	-
MZ182_RS14095 (MZ182_14095)	proC	3153319..3154137 (+)	819	WP_248065811.1	pyrroline-5-carboxylate reductase	-
MZ182_RS14100 (MZ182_14100)	-	3154184..3154732 (+)	549	WP_011788671.1	YggT family protein	-
MZ182_RS14105 (MZ182_14105)	yggU	3154732..3155022 (+)	291	WP_011788670.1	DUF167 family protein YggU	-
MZ182_RS14110 (MZ182_14110)	-	3155100..3155534 (+)	435	WP_011788669.1	DUF4426 domain-containing protein	-
MZ182_RS14115 (MZ182_14115)	rdgB	3155645..3156262 (+)	618	WP_011919679.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-

Sequence

Protein

Download Length: 370 a.a. Molecular weight: 41098.22 Da Isoelectric Point: 6.0920

                                >NTDB_id=680192 MZ182_RS14080 WP_248065812.1 3150245..3151357(-) (pilU) [Shewanella sp. JNE2]

MDVRPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR

FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED

PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI

MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA

LLELYVDGEISYADALHHADSPNDLRLMIKLQNKEPTGSSFMEGVTLDMD

Nucleotide

Download Length: 1113 bp

                                >NTDB_id=680192 MZ182_RS14080 WP_248065812.1 3150245..3151357(-) (pilU) [Shewanella sp. JNE2]

ATGGATGTACGTCCGTTTTTAAAAGTCATCGTTGAGCGTAAAGCCTCTGATTTGTTTATTACCGCGGGCTTTCCTCCCAG

TGCAAAAATTGATGGCGAATTACGTCCCCTCGCCGAGAGTGCATTTACGCCTGCACAGTCTTTAGATTTTGTTGAGTCAC

TCATGACTGAAGCGCAGAAACAGGAATTTCACTCAACCCGTGAATGTAACTTTGCTTTTGCGGCAAAGGATCTCGGTCGT

TTCCGGGTCAGCGCTTTTTGGCAAAGGGAATCACCAGGATGTGTTATGCGCCGGATTGAGACCAAAATTCCCGAAGTCGA

TGACTTAAAATTACCTCCTATTTTAAAAGATCTGGTGATGAGCAAGCGTGGGCTTATCATTATGGTCGGCGGCACAGGTA

CGGGTAAGTCAACCTCATTAGCAGCATTGGTTGGATACCGTAATGCCCATGCCCGTGGCCATATATTGACGATTGAAGAT

CCTGTTGAATTTGTTCACGATCATAGAAAAAGCATTATCACCCAACGTGAAGTAGGCATAGATACTGAGTCATTCGATGC

GGCATTAAAAAGCTCCCTTCGTCAGGCACCCGATGTGATCTTAATCGGTGAAATTCGTACCCAAGAGACGATGGAATTTG

CATTGTCGTTTGCAGAAACAGGTCATCTTTGTATGGCAACCCTACATGCCAATAATGCGAACCAAGCACTCGATCGGATT

ATGCACTTAGTGCCTGAGAGTAAACATCAGCAGTTACTATTTGATTTATCCTTAAATTTACGTGGTATTGTGGCGCAGCA

ATTGATCCCCAAAGTCGATGGAACGGGGCGCCGCGCCGCGATTGAAGTATTAATTAATACTCCAAGGGTAGCGAGTTTAA

TTGCCAAAAATGAGCTGCATTTACTCAAGGAAACCATGGCAAAATCGAATGAACAGGGGATGCAGACATTCGACCAAGCG

TTACTGGAATTATATGTTGATGGTGAAATTAGTTATGCCGATGCCTTACACCATGCTGACTCACCAAACGATTTACGTTT

GATGATCAAATTACAAAATAAAGAGCCTACTGGTTCAAGTTTTATGGAGGGCGTAACACTCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilU	Pseudomonas stutzeri DSM 10701	60.215	100	0.605
pilU	Vibrio cholerae strain A1552	58.649	100	0.586
pilU	Acinetobacter baylyi ADP1	61.08	95.135	0.581
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.87	93.243	0.381
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	42.727	89.189	0.381
pilT	Vibrio cholerae strain A1552	42.727	89.189	0.381
pilT	Legionella pneumophila strain Lp02	40.785	89.459	0.365
pilT	Legionella pneumophila strain ERS1305867	40.785	89.459	0.365
pilT	Acinetobacter baumannii D1279779	39.181	92.432	0.362
pilT	Acinetobacter baumannii strain A118	39.181	92.432	0.362
pilT	Acinetobacter nosocomialis M2	39.181	92.432	0.362

Detailed information NTG0680192