Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MW889_RS02625 Genome accession   NZ_CP095867
Coordinates   532165..533202 (-) Length   345 a.a.
NCBI ID   WP_006581387.1    Uniprot ID   A0A0A7XL61
Organism   Acinetobacter nosocomialis strain 11J360     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 527165..538202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW889_RS02595 (MW889_02605) - 527948..528403 (+) 456 WP_000782976.1 bacteriohemerythrin -
  MW889_RS02600 (MW889_02610) - 528556..529623 (+) 1068 WP_033846680.1 membrane protein -
  MW889_RS02605 (MW889_02615) - 529623..529946 (+) 324 WP_002052062.1 RnfH family protein -
  MW889_RS02610 (MW889_02620) bamE 530002..530400 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  MW889_RS02615 (MW889_02625) fur 530512..530949 (+) 438 WP_002052029.1 ferric iron uptake transcriptional regulator -
  MW889_RS02620 (MW889_02630) pilU 531019..532137 (-) 1119 WP_002052019.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MW889_RS02625 (MW889_02635) pilT 532165..533202 (-) 1038 WP_006581387.1 type IV pilus twitching motility protein PilT Machinery gene
  MW889_RS02630 (MW889_02640) - 533329..534021 (+) 693 WP_032010894.1 YggS family pyridoxal phosphate-dependent enzyme -
  MW889_RS02635 (MW889_02645) - 534129..534971 (+) 843 WP_033846643.1 undecaprenyl-diphosphate phosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38328.92 Da        Isoelectric Point: 6.7271

>NTDB_id=678870 MW889_RS02625 WP_006581387.1 532165..533202(-) (pilT) [Acinetobacter nosocomialis strain 11J360]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=678870 MW889_RS02625 WP_006581387.1 532165..533202(-) (pilT) [Acinetobacter nosocomialis strain 11J360]
ATGGATATTACAGAGCTACTTGCCTTCTCTGTGAAAAATGGCGCATCCGATTTGCACTTATCAGCTGGTATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATTAACTTGCCAGCCTTAGAACATAAAGATGTCCACCGCCTCGTTTATGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCGAATGTTGCGCGT
TTCCGTGTGAATGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGGCAAATCTTTAAAGATATTTGCGACTATCCTCGCGGTATTGTATTGGTCACAGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCAATGCTCGATTATATTAATGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACATCAGTCCAAGAAATGTCTGATTAACCAGCGTGAAGTGCATCGTGATACACATGGCTTTAATGA
AGCCCTTCGCTCAGCACTACGTGAAGACCCAGATATTATTTTAGTCGGTGAGATGCGTGACCTTGAAACGATTCGATTAG
CACTTACTGCTGCCGAAACAGGTCACTTGGTATTTGGTACGCTTCACACCACATCGGCTGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAAGCAGTTATTTCTCAGAC
CCTGCTTAAAAAGAATGGTGGTGGTCGTGTTGCGGCACATGAAATTATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTATTCAGCTATTCAGACTGGTGCTAACCATGGCATGACAACCCTCGACCAAAGCCTA
AAAGGTTTGGTTTCACGTGGTGTAATTAGTCCACAAACAGCCCGTACAGCGGCGAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A7XL61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

100

100

1
  pilT Acinetobacter baumannii D1279779

99.71

100

0.997
  pilT Acinetobacter baumannii strain A118

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388