Detailed information NTG0678869

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MW889_RS02620
• Genome accession: NZ_CP095867
• Coordinates: 531019..532137 (-)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis strain 11J360
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 526019..537137

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MW889_RS02590 (MW889_02600)	-	526847..527695 (+)	849	WP_022575107.1	class II glutamine amidotransferase	-
MW889_RS02595 (MW889_02605)	-	527948..528403 (+)	456	WP_000782976.1	bacteriohemerythrin	-
MW889_RS02600 (MW889_02610)	-	528556..529623 (+)	1068	WP_033846680.1	membrane protein	-
MW889_RS02605 (MW889_02615)	-	529623..529946 (+)	324	WP_002052062.1	RnfH family protein	-
MW889_RS02610 (MW889_02620)	bamE	530002..530400 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
MW889_RS02615 (MW889_02625)	fur	530512..530949 (+)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
MW889_RS02620 (MW889_02630)	pilU	531019..532137 (-)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MW889_RS02625 (MW889_02635)	pilT	532165..533202 (-)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
MW889_RS02630 (MW889_02640)	-	533329..534021 (+)	693	WP_032010894.1	YggS family pyridoxal phosphate-dependent enzyme	-
MW889_RS02635 (MW889_02645)	-	534129..534971 (+)	843	WP_033846643.1	undecaprenyl-diphosphate phosphatase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=678869 MW889_RS02620 WP_002052019.1 531019..532137(-) (pilU) [Acinetobacter nosocomialis strain 11J360]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=678869 MW889_RS02620 WP_002052019.1 531019..532137(-) (pilU) [Acinetobacter nosocomialis strain 11J360]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTGATTGGACAGCTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCCATCATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGCATTATTACTCAACGTGAAGTCGGTATTGATACGGACTCATTCGA
AATTGCGTTAAAAAACACGTTACGACAAGCGCCAGATGTGATCTTAATTGGTGAAATCCGCTCTCGTGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGACACCTTGTATTAGCTACAATGCATGCCAACAATGCCAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
ACAGCTTATTCCAACACCAGATGGTAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATACAAAGCAGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAACTTGCAAATGAAGGGCCTGATCAAATTACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403