Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L2Y96_RS16460 Genome accession   NZ_CP095741
Coordinates   3700188..3701225 (-) Length   345 a.a.
NCBI ID   WP_247328320.1    Uniprot ID   -
Organism   Luteibacter aegosomaticola strain 335     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3695188..3706225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y96_RS16440 (L2Y96_16440) - 3695436..3696362 (+) 927 WP_247328312.1 aspartate carbamoyltransferase catalytic subunit -
  L2Y96_RS16445 (L2Y96_16445) - 3696439..3698187 (+) 1749 WP_247328314.1 hypothetical protein -
  L2Y96_RS16450 (L2Y96_16450) - 3698427..3698792 (+) 366 WP_247328316.1 BON domain-containing protein -
  L2Y96_RS16455 (L2Y96_16455) pilU 3698874..3700037 (-) 1164 WP_247328318.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Y96_RS16460 (L2Y96_16460) pilT 3700188..3701225 (-) 1038 WP_247328320.1 type IV pilus twitching motility protein PilT Machinery gene
  L2Y96_RS16465 (L2Y96_16465) proC 3701343..3702167 (+) 825 WP_247328321.1 pyrroline-5-carboxylate reductase -
  L2Y96_RS16470 (L2Y96_16470) - 3702164..3702739 (+) 576 WP_247328323.1 YggT family protein -
  L2Y96_RS16475 (L2Y96_16475) - 3702745..3704868 (-) 2124 WP_247328325.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38488.20 Da        Isoelectric Point: 6.7837

>NTDB_id=677984 L2Y96_RS16460 WP_247328320.1 3700188..3701225(-) (pilT) [Luteibacter aegosomaticola strain 335]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPPLEHKQVHSLIYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNHNRGAGGVFRTIPSEVLTLEDLGAPKIFRELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHLLTVED
PIEFVHTSQKCLVNQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSAIQTGQQYGMQTLDQNL
ADLVKRGLITRQEGMMYAKNKEGFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=677984 L2Y96_RS16460 WP_247328320.1 3700188..3701225(-) (pilT) [Luteibacter aegosomaticola strain 335]
ATGGATATCGCCGAACTCCTTGCCTTCTCAGTGAAGAACAAGGCCTCCGACCTGCATCTTTCCGCAGGCCTGCCGCCCAT
GATCCGCGTGGACGGTGACGTTCGACGGATCAATATCCCGCCCCTGGAGCACAAGCAGGTCCATTCGCTGATTTACGACA
TCATGTCGGATAAGCAGCGCCGCGATTATGAAGAATTCCTCGAGGTCGACTTCTCGTTCGAGATCCCCGGCCTGGCCCGC
TTCCGCGTCAACGCGTTCAACCACAACCGTGGTGCCGGTGGCGTGTTCCGTACCATTCCTTCGGAAGTGCTTACCCTCGA
AGACCTGGGTGCGCCCAAGATCTTCCGCGAGCTGATCGAACAGCCGCAGGGGTTGATCCTGGTCACGGGCCCCACGGGTT
CGGGTAAATCCACGACCCTGGCCGCGATGGTCGACCACATCAACAAGAACGAATACGGCCATCTCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGGTTAACCAGCGCGAGGTTCACCGCGATACGCACGGATTCAACGA
AGCGCTGCGTTCCGCGCTGCGTGAGGATCCCGACTACATCCTGGTGGGCGAGCTTCGCGACCTGGAAACCATCCGCCTGG
CCCTCACCGCCGCGGAAACGGGACATCTTGTGTTCGGCACCCTGCATACCTCGTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCGGGCGAAAAGCCGATGGTCCGCTCGATGCTTTCCGAGTCGCTGCGCGCGGTTATTTCGCAGTC
GCTGCTGAAGAAGGTGGGCGGTGGCCGTATTGCCGCGCACGAGATCATGGTGGGCATCCCGGCTATCCGAAACCTGATCC
GCGAGGACAAGGTGGCCCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGCATGCAGACCCTCGACCAGAACCTG
GCCGACCTGGTCAAGCGCGGCCTCATCACCCGCCAGGAAGGCATGATGTACGCCAAGAACAAGGAAGGCTTCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

77.907

99.71

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.71

0.774
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.871

96.522

0.684
  pilT Vibrio cholerae strain A1552

70.871

96.522

0.684
  pilT Neisseria meningitidis 8013

64.638

100

0.646
  pilT Neisseria gonorrhoeae MS11

64.348

100

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

39.595

100

0.397
  pilU Vibrio cholerae strain A1552

38.728

100

0.388
  pilU Acinetobacter baylyi ADP1

38.329

100

0.386