Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L2Y96_RS16455 Genome accession   NZ_CP095741
Coordinates   3698874..3700037 (-) Length   387 a.a.
NCBI ID   WP_247328318.1    Uniprot ID   -
Organism   Luteibacter aegosomaticola strain 335     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3693874..3705037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y96_RS16425 (L2Y96_16425) - 3693992..3694318 (-) 327 WP_247328306.1 hypothetical protein -
  L2Y96_RS16430 (L2Y96_16430) - 3694410..3694976 (+) 567 WP_247328308.1 YqgE/AlgH family protein -
  L2Y96_RS16435 (L2Y96_16435) ruvX 3694980..3695399 (+) 420 WP_247328310.1 Holliday junction resolvase RuvX -
  L2Y96_RS16440 (L2Y96_16440) - 3695436..3696362 (+) 927 WP_247328312.1 aspartate carbamoyltransferase catalytic subunit -
  L2Y96_RS16445 (L2Y96_16445) - 3696439..3698187 (+) 1749 WP_247328314.1 hypothetical protein -
  L2Y96_RS16450 (L2Y96_16450) - 3698427..3698792 (+) 366 WP_247328316.1 BON domain-containing protein -
  L2Y96_RS16455 (L2Y96_16455) pilU 3698874..3700037 (-) 1164 WP_247328318.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Y96_RS16460 (L2Y96_16460) pilT 3700188..3701225 (-) 1038 WP_247328320.1 type IV pilus twitching motility protein PilT Machinery gene
  L2Y96_RS16465 (L2Y96_16465) proC 3701343..3702167 (+) 825 WP_247328321.1 pyrroline-5-carboxylate reductase -
  L2Y96_RS16470 (L2Y96_16470) - 3702164..3702739 (+) 576 WP_247328323.1 YggT family protein -
  L2Y96_RS16475 (L2Y96_16475) - 3702745..3704868 (-) 2124 WP_247328325.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43338.60 Da        Isoelectric Point: 6.9972

>NTDB_id=677983 L2Y96_RS16455 WP_247328318.1 3698874..3700037(-) (pilU) [Luteibacter aegosomaticola strain 335]
MSEFDFTSFLKLMVHKQASDLFITAGVAPSMKVQGRIVPITQSPLSAQQSRDMVLNVMTPAQREEFEKTHECQFAISASG
VGRFRVSCFYQRNCVGMVLRRIESKIPTPEELNLPPIIKQLAMTKRGIIIFVGGTGTGKSTSLASMIGYRNQNSTGHIIT
IEDPIEYVHRHEGCIITQRELGIDTDNWENALKNTLRQAPDVIMIGEVRTRETMEHAINFSETGHLCLCTLHANNANQAL
DRILHFFPEDRRSALFMDLSLNLKGIVAQQLIPTPDGKSRRVAVEVLLGTPLAQDYIRQGEIHKLKELMRDSNQLGMRTF
DQSLVELYHAGEISYEDALRHADSSNEVRLRIKLAQGGDAHTLSQGLEGVEIDKANDKNTFGGMLHR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=677983 L2Y96_RS16455 WP_247328318.1 3698874..3700037(-) (pilU) [Luteibacter aegosomaticola strain 335]
ATGAGCGAGTTCGATTTCACCTCCTTTCTGAAGCTGATGGTGCATAAACAGGCGTCCGACCTGTTCATCACCGCTGGCGT
CGCACCTTCCATGAAGGTCCAGGGCCGCATCGTCCCGATCACACAGAGCCCCCTGTCCGCGCAGCAGTCGCGCGACATGG
TGCTTAACGTGATGACGCCGGCCCAGCGCGAAGAGTTCGAAAAGACCCACGAATGCCAGTTCGCGATCAGCGCCTCGGGC
GTCGGCCGCTTCCGTGTGTCGTGCTTCTACCAGCGCAACTGCGTGGGCATGGTCCTGCGCCGCATCGAGTCGAAGATCCC
GACGCCGGAAGAACTGAATCTGCCGCCGATCATCAAGCAGCTGGCGATGACCAAGCGCGGCATCATCATTTTCGTGGGCG
GTACCGGTACCGGTAAATCCACCTCGCTTGCCTCGATGATCGGTTACCGCAACCAGAATTCGACGGGCCACATCATCACG
ATCGAAGACCCGATCGAATACGTGCACCGCCATGAAGGCTGCATCATCACCCAGCGCGAACTCGGCATCGATACCGACAA
CTGGGAAAACGCGCTGAAGAACACCCTGCGCCAGGCGCCTGACGTGATCATGATCGGCGAAGTGCGTACGCGTGAAACGA
TGGAGCACGCGATCAACTTCTCCGAAACGGGCCACCTGTGCCTGTGTACGCTGCACGCGAACAACGCCAACCAGGCCCTC
GACCGCATCCTGCACTTCTTCCCGGAAGACCGCCGCAGCGCGTTGTTCATGGATCTTTCGCTGAACCTGAAGGGCATCGT
GGCCCAGCAGCTCATCCCGACGCCCGATGGCAAGTCCCGCCGCGTCGCGGTGGAGGTGCTGCTTGGTACGCCGCTCGCGC
AGGATTACATCCGGCAGGGCGAGATCCACAAGCTCAAGGAGCTCATGCGCGATTCGAACCAGCTGGGCATGCGCACCTTC
GACCAGAGCCTCGTCGAGCTGTACCACGCCGGTGAGATCTCGTACGAAGACGCGCTGCGTCATGCCGACAGCTCGAACGA
GGTGCGCCTGCGCATCAAGCTCGCACAGGGGGGCGATGCCCACACGCTGTCACAGGGCCTCGAAGGCGTGGAAATTGACA
AGGCCAACGACAAGAACACCTTCGGCGGCATGCTCCACCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.508

96.641

0.633
  pilU Acinetobacter baylyi ADP1

63.483

91.99

0.584
  pilU Vibrio cholerae strain A1552

51.081

95.607

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.667

96.899

0.375