Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L2Y94_RS06195 Genome accession   NZ_CP095740
Coordinates   1346109..1347146 (+) Length   345 a.a.
NCBI ID   WP_247373786.1    Uniprot ID   -
Organism   Luteibacter aegosomatis strain 321     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1341109..1352146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y94_RS06180 (L2Y94_06180) - 1342356..1344464 (+) 2109 WP_247373780.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  L2Y94_RS06185 (L2Y94_06185) - 1344594..1345169 (-) 576 WP_247373782.1 YggT family protein -
  L2Y94_RS06190 (L2Y94_06190) proC 1345166..1345990 (-) 825 WP_247373784.1 pyrroline-5-carboxylate reductase -
  L2Y94_RS06195 (L2Y94_06195) pilT 1346109..1347146 (+) 1038 WP_247373786.1 type IV pilus twitching motility protein PilT Machinery gene
  L2Y94_RS06200 (L2Y94_06200) pilU 1347272..1348438 (+) 1167 WP_247373787.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Y94_RS06205 (L2Y94_06205) - 1348641..1349006 (-) 366 WP_247373789.1 BON domain-containing protein -
  L2Y94_RS06210 (L2Y94_06210) - 1349232..1350980 (-) 1749 WP_247373790.1 hypothetical protein -
  L2Y94_RS06215 (L2Y94_06215) - 1351083..1352009 (-) 927 WP_247373792.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38474.18 Da        Isoelectric Point: 6.7837

>NTDB_id=677955 L2Y94_RS06195 WP_247373786.1 1346109..1347146(+) (pilT) [Luteibacter aegosomatis strain 321]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHALIYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNHNRGAGGVFRTIPSEVLTLEDLGAPRIFRELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHMLTVED
PIEFVHTSQKCLVNQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSAIQTGQQYGMQTLDQNL
LDLVKRGLITRQEGAMYAKNKEGFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=677955 L2Y94_RS06195 WP_247373786.1 1346109..1347146(+) (pilT) [Luteibacter aegosomatis strain 321]
ATGGACATCGCCGAACTCCTTGCCTTCTCCGTGAAGAACAAGGCCTCCGACCTGCACCTTTCCGCGGGTCTTCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGCATCAACATCCCCGCGCTGGAGCACAAGCAGGTCCATGCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGTCGCGACTACGAGGAGTTCCTCGAGGTCGACTTCTCCTTCGAGATCCCCGGCCTGGCCCGC
TTCCGCGTCAACGCCTTCAACCACAACCGCGGCGCGGGCGGCGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTGGA
AGACCTGGGCGCCCCGCGCATCTTCCGCGAACTGATCGAGCAGCCGCAGGGCCTGATCCTGGTCACGGGACCCACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACCACATCAACAAGAACGAATACGGTCACATGCTGACCGTGGAAGAC
CCCATCGAATTCGTGCATACCTCGCAGAAGTGCCTGGTGAACCAGCGCGAGGTGCATCGCGACACCCATGGCTTCAACGA
GGCGCTGCGTTCGGCGTTGCGCGAAGATCCCGACTACATCCTCGTGGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTCACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCGGGCGAGAAGCCCATGGTCCGCTCCATGCTCTCCGAGTCGTTGCGCGCGGTGATCTCGCAGTC
GCTTTTGAAGAAGGTGGGCGGCGGGCGCATCGCGGCGCACGAGATCATGGTGGGCATCCCGGCCATTCGTAACCTCATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGCATGCAGACGCTCGACCAGAACCTG
CTCGACCTGGTCAAGCGTGGCCTCATCACGCGCCAGGAAGGCGCGATGTACGCCAAGAACAAGGAAGGTTTCCGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

77.907

99.71

0.777
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.171

96.522

0.687
  pilT Vibrio cholerae strain A1552

71.171

96.522

0.687
  pilT Neisseria meningitidis 8013

64.638

100

0.646
  pilT Neisseria gonorrhoeae MS11

64.348

100

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

39.306

100

0.394
  pilU Vibrio cholerae strain A1552

39.306

100

0.394
  pilU Acinetobacter baylyi ADP1

38.329

100

0.386