Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HH1059_RS07500 Genome accession   NZ_AP017372
Coordinates   1640383..1641417 (-) Length   344 a.a.
NCBI ID   WP_096409604.1    Uniprot ID   A0A0X8XB68
Organism   Halorhodospira halochloris strain DSM 1059     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1635383..1646417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH1059_RS07480 (HH1059_14930) - 1635652..1637991 (+) 2340 WP_096409600.1 ATP-binding protein -
  HH1059_RS07490 (HH1059_14950) - 1638569..1638985 (+) 417 WP_096409602.1 hypothetical protein -
  HH1059_RS07495 (HH1059_14960) pilU 1639223..1640371 (-) 1149 WP_096409603.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HH1059_RS07500 (HH1059_14970) pilT 1640383..1641417 (-) 1035 WP_096409604.1 type IV pilus twitching motility protein PilT Machinery gene
  HH1059_RS07505 (HH1059_14980) - 1641433..1642458 (-) 1026 WP_096409605.1 succinylglutamate desuccinylase/aspartoacylase family protein -
  HH1059_RS07510 (HH1059_14990) rimK 1642524..1643432 (-) 909 WP_096409606.1 30S ribosomal protein S6--L-glutamate ligase -
  HH1059_RS07515 (HH1059_15000) - 1643619..1644083 (-) 465 WP_096409607.1 ATP-dependent zinc protease -
  HH1059_RS07520 (HH1059_15010) - 1644178..1644705 (+) 528 WP_096409608.1 hypothetical protein -
  HH1059_RS07525 (HH1059_15020) - 1644955..1645926 (-) 972 WP_096409609.1 SLAC1 anion channel family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38352.83 Da        Isoelectric Point: 6.3479

>NTDB_id=67787 HH1059_RS07500 WP_096409604.1 1640383..1641417(-) (pilT) [Halorhodospira halochloris strain DSM 1059]
MDVTELLDFAVKNNASDLHLSAGMPPLIRVDGDVRRIDVPEMDHKSVQALVYDIMNDRQRREFEENLEIDFSFAVPQLSR
FRVNVFNQDRGIGAVFRAIPSQVVTLEDIQAPEIFRTLCDRPRGLILVTGPTGSGKSTTLAAMVDHKNRNEYGHILTVED
PIEFVHQPKRCIVNQREVHRDTHSFNSALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAPKTVDRI
IDVFPAAEKTMVRSMLSESLEAVISQTLIKKVGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGQSMGMQTLDQSL
QKLVQTGQITREDAKSKALSKDIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=67787 HH1059_RS07500 WP_096409604.1 1640383..1641417(-) (pilT) [Halorhodospira halochloris strain DSM 1059]
ATGGATGTAACAGAACTATTGGATTTCGCCGTAAAAAACAACGCATCAGATCTGCACCTTTCAGCCGGGATGCCACCTCT
GATCCGCGTTGATGGTGACGTGCGCCGCATTGATGTTCCGGAAATGGACCATAAGTCGGTCCAGGCCTTAGTTTACGACA
TAATGAATGACCGCCAGCGGCGCGAATTTGAAGAGAACCTGGAGATCGACTTTTCATTTGCTGTGCCTCAGCTATCCCGG
TTTAGGGTCAACGTCTTCAATCAGGATCGGGGTATCGGAGCTGTATTCAGAGCGATACCTTCGCAGGTCGTCACCCTTGA
AGATATTCAGGCGCCGGAGATCTTTAGGACCCTATGCGATCGACCGCGTGGGCTTATCTTGGTCACTGGACCGACAGGCT
CGGGTAAATCAACAACCCTTGCGGCGATGGTGGACCATAAAAATCGTAATGAGTATGGCCACATCTTGACCGTTGAAGAT
CCTATAGAATTTGTCCATCAGCCTAAACGCTGTATAGTCAATCAGCGCGAGGTTCATCGCGATACCCACAGCTTCAACAG
CGCTCTGCGCTCGGCACTGCGCGAAGACCCTGATGTGATACTGGTTGGTGAGTTACGCGATTTGGAGACAATTCGGCTCG
CTTTGACCGCAGCAGAGACTGGTCACCTGGTATTTGGTACGTTGCACACCAGCTCGGCCCCGAAGACGGTGGATCGCATA
ATCGATGTCTTCCCTGCGGCGGAGAAGACCATGGTTCGTTCAATGCTCTCCGAATCCTTGGAAGCGGTTATTTCGCAGAC
CTTGATCAAGAAGGTCGGAGGAGGGCGGGTTGCAGCGCACGAGATCATGCTTGGCACCTCTGCGATTCGTAACCTGATAC
GTGAGGATAAGGTTGCGCAGATGTACTCCTCTATTCAAACCGGCCAGTCTATGGGCATGCAGACCCTTGATCAGTCATTA
CAGAAACTGGTTCAAACCGGCCAAATAACTCGCGAAGACGCGAAGTCAAAGGCGTTGAGCAAAGATATATTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X8XB68

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

74.419

100

0.744
  pilT Pseudomonas stutzeri DSM 10701

74.128

100

0.741
  pilT Acinetobacter baumannii D1279779

73.547

100

0.735
  pilT Acinetobacter baumannii strain A118

73.547

100

0.735
  pilT Acinetobacter nosocomialis M2

73.547

100

0.735
  pilT Pseudomonas aeruginosa PAK

73.964

98.256

0.727
  pilT Legionella pneumophila strain Lp02

69.477

100

0.695
  pilT Legionella pneumophila strain ERS1305867

69.477

100

0.695
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.656

97.965

0.663
  pilT Vibrio cholerae strain A1552

67.656

97.965

0.663
  pilT Neisseria meningitidis 8013

64.012

98.547

0.631
  pilT Neisseria gonorrhoeae MS11

63.717

98.547

0.628
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.497
  pilU Vibrio cholerae strain A1552

42.274

99.709

0.422
  pilU Pseudomonas stutzeri DSM 10701

42.687

97.384

0.416
  pilU Acinetobacter baylyi ADP1

40

98.837

0.395

Multiple sequence alignment