Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HH1059_RS07495 Genome accession   NZ_AP017372
Coordinates   1639223..1640371 (-) Length   382 a.a.
NCBI ID   WP_096409603.1    Uniprot ID   A0A0X8XAU3
Organism   Halorhodospira halochloris strain DSM 1059     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1634223..1645371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH1059_RS07480 (HH1059_14930) - 1635652..1637991 (+) 2340 WP_096409600.1 ATP-binding protein -
  HH1059_RS07490 (HH1059_14950) - 1638569..1638985 (+) 417 WP_096409602.1 hypothetical protein -
  HH1059_RS07495 (HH1059_14960) pilU 1639223..1640371 (-) 1149 WP_096409603.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HH1059_RS07500 (HH1059_14970) pilT 1640383..1641417 (-) 1035 WP_096409604.1 type IV pilus twitching motility protein PilT Machinery gene
  HH1059_RS07505 (HH1059_14980) - 1641433..1642458 (-) 1026 WP_096409605.1 succinylglutamate desuccinylase/aspartoacylase family protein -
  HH1059_RS07510 (HH1059_14990) rimK 1642524..1643432 (-) 909 WP_096409606.1 30S ribosomal protein S6--L-glutamate ligase -
  HH1059_RS07515 (HH1059_15000) - 1643619..1644083 (-) 465 WP_096409607.1 ATP-dependent zinc protease -
  HH1059_RS07520 (HH1059_15010) - 1644178..1644705 (+) 528 WP_096409608.1 hypothetical protein -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 41775.39 Da        Isoelectric Point: 4.9677

>NTDB_id=67786 HH1059_RS07495 WP_096409603.1 1639223..1640371(-) (pilU) [Halorhodospira halochloris strain DSM 1059]
MQLDFLLDQLTQRKGSDLFITAGSAPLIKVGGALQALADAPLGIDEVARLVHESMNQEQVAEFEQQQEANFAIVNETGGR
FRVSAFYQRGNPGMVIRRIETTIPDLDELQLPPIIHDLAMTKRGLIMFVGGTGTGKSTSLAAMLGKRNSTAPGHILTIED
PVEFVHPHRQCIVTQREVGVDTQSFDAALKNALRQAPDVILIGEVRSREIMDYAVSFAETGHLVLATLHANNANQALDRI
INFFPSERREQLLMDLSLNLKAVVAQQLIPSSSGHGRVAALEVLLATPLVKEKIRSGDIHELKEIMSSSEEQGMQTFDRH
LFRLYRDGFISYENALAHADSANEVRLMAKLEGLETGGVGPEGSESDDDRPRGGSGLSLADY

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=67786 HH1059_RS07495 WP_096409603.1 1639223..1640371(-) (pilU) [Halorhodospira halochloris strain DSM 1059]
ATGCAGTTAGATTTCTTGTTGGATCAGCTAACTCAGCGCAAGGGCTCCGATCTGTTCATTACCGCAGGTTCAGCGCCCTT
GATCAAAGTGGGTGGTGCTTTGCAGGCCCTTGCCGATGCTCCTCTTGGAATTGATGAGGTGGCCAGACTGGTGCATGAGA
GCATGAATCAGGAGCAGGTAGCTGAGTTTGAGCAGCAACAGGAAGCCAATTTCGCCATTGTGAATGAAACAGGCGGGCGA
TTCCGGGTTAGCGCTTTTTATCAGCGCGGCAACCCTGGCATGGTGATCAGGCGTATTGAAACCACTATACCTGACCTTGA
CGAACTGCAGTTACCCCCGATTATCCATGACTTAGCCATGACCAAGCGCGGTCTGATCATGTTTGTCGGGGGGACGGGGA
CCGGCAAATCGACATCGCTGGCGGCTATGCTCGGCAAGCGCAACTCCACCGCCCCTGGACACATACTGACGATAGAGGAT
CCGGTAGAGTTTGTTCATCCTCATCGACAATGCATTGTGACTCAACGCGAAGTCGGGGTGGATACCCAATCCTTTGATGC
AGCCCTTAAAAATGCCCTGCGTCAGGCCCCTGACGTGATATTGATTGGTGAAGTACGTTCGCGGGAGATCATGGATTACG
CCGTCTCATTTGCCGAGACAGGGCATTTGGTCTTGGCAACACTCCACGCTAACAATGCCAACCAGGCCCTGGATCGGATC
ATAAACTTCTTTCCTAGCGAGCGCCGTGAGCAGCTACTGATGGATCTATCCCTAAACCTCAAGGCGGTTGTTGCCCAACA
GCTTATACCGAGCTCTTCTGGTCACGGCAGAGTGGCAGCGCTCGAAGTGCTTTTGGCTACCCCTCTGGTTAAAGAGAAGA
TTCGCAGTGGTGATATACATGAACTCAAGGAGATTATGAGCTCATCGGAGGAGCAAGGGATGCAGACTTTTGATCGCCAC
CTGTTCCGGCTGTATCGCGATGGGTTCATCTCATATGAAAACGCGCTCGCCCATGCCGATTCGGCTAACGAGGTTAGGCT
GATGGCCAAACTCGAAGGGCTAGAGACCGGTGGCGTTGGGCCTGAGGGCAGTGAAAGCGATGATGATAGACCGCGCGGAG
GGTCGGGCCTCAGTTTGGCGGATTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X8XAU3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.173

93.717

0.573
  pilU Acinetobacter baylyi ADP1

59.943

92.147

0.552
  pilU Vibrio cholerae strain A1552

56.944

94.241

0.537
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.759

92.408

0.414
  pilT Legionella pneumophila strain Lp02

42.687

87.696

0.374
  pilT Legionella pneumophila strain ERS1305867

42.687

87.696

0.374
  pilT Neisseria gonorrhoeae MS11

40.351

89.529

0.361
  pilT Neisseria meningitidis 8013

40.351

89.529

0.361

Multiple sequence alignment