Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LGT42_RS03560 Genome accession   NZ_CP095733
Coordinates   738736..739773 (+) Length   345 a.a.
NCBI ID   WP_226071550.1    Uniprot ID   -
Organism   Methylophaga pinxianii strain TMB1265     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 733736..744773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGT42_RS03530 (LGT42_003530) pyrE 734083..734724 (-) 642 WP_226071541.1 orotate phosphoribosyltransferase -
  LGT42_RS03535 (LGT42_003535) metW 734721..735323 (-) 603 WP_226071542.1 methionine biosynthesis protein MetW -
  LGT42_RS03540 (LGT42_003540) - 735323..736471 (-) 1149 WP_226071543.1 homoserine O-acetyltransferase -
  LGT42_RS03545 (LGT42_003545) - 736496..737071 (-) 576 WP_226071544.1 YggT family protein -
  LGT42_RS03550 (LGT42_003550) proC 737064..737900 (-) 837 WP_226071548.1 pyrroline-5-carboxylate reductase -
  LGT42_RS03555 (LGT42_003555) - 737947..738645 (-) 699 WP_226071549.1 YggS family pyridoxal phosphate-dependent enzyme -
  LGT42_RS03560 (LGT42_003560) pilT 738736..739773 (+) 1038 WP_226071550.1 type IV pilus twitching motility protein PilT Machinery gene
  LGT42_RS03565 (LGT42_003565) pilU 739783..740925 (+) 1143 WP_226071597.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LGT42_RS03570 (LGT42_003570) - 741099..741950 (+) 852 WP_226071551.1 OmpA family protein -
  LGT42_RS03575 (LGT42_003575) - 742346..742540 (+) 195 WP_349630199.1 CsbD family protein -
  LGT42_RS03580 (LGT42_003580) - 742801..743364 (+) 564 WP_226071552.1 BON domain-containing protein -
  LGT42_RS03585 (LGT42_003585) - 743372..743833 (+) 462 WP_226071553.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38665.34 Da        Isoelectric Point: 6.6408

>NTDB_id=677786 LGT42_RS03560 WP_226071550.1 738736..739773(+) (pilT) [Methylophaga pinxianii strain TMB1265]
MDITELLTFSAKNNASDLHISAGEPPMIRVDGDIKRINLPAMEHKEVHAMVYDIMNDRQRKDFEEFLEADFSFEIPNLAR
FRVNVFNQNRGAAAVFRTIPSTVLTLEELGAPEIFKQIADNHRGIVLVTGPTGSGKSTTLAAMINYRNEKDNEHILTIED
PIEFVHRSKKCLINQREVHRDTHGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMIGTPAIRNLIREDKVPQMYSSIQTGGALGMQTLDQCL
QDLLRRQQITKHEALPRAVNKDLFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=677786 LGT42_RS03560 WP_226071550.1 738736..739773(+) (pilT) [Methylophaga pinxianii strain TMB1265]
ATGGATATTACTGAATTGCTGACCTTTAGTGCAAAAAACAATGCGTCGGATTTGCACATTTCTGCGGGTGAGCCGCCTAT
GATACGTGTCGATGGTGATATAAAGCGGATTAACTTGCCAGCCATGGAACACAAAGAAGTGCATGCCATGGTGTACGACA
TCATGAATGACCGGCAGCGCAAGGACTTTGAGGAATTTCTGGAAGCGGATTTTTCGTTCGAAATTCCTAATCTGGCCCGT
TTCCGGGTGAATGTGTTTAATCAGAACCGCGGTGCCGCTGCTGTTTTCCGTACCATCCCTTCAACCGTACTAACGTTGGA
AGAATTGGGGGCTCCCGAGATTTTCAAACAAATTGCTGATAACCATCGCGGCATTGTGTTGGTTACTGGCCCTACCGGTT
CCGGTAAATCAACGACATTGGCTGCGATGATTAATTATCGCAATGAAAAAGATAATGAACACATCCTGACCATTGAAGAT
CCCATCGAATTTGTGCACCGCAGTAAGAAGTGCCTGATTAACCAGCGTGAAGTGCATCGTGATACGCATGGTTTCAGTGA
AGCCTTAAGAAGTGCTTTGCGTGAAGATCCAGACATTATTCTGGTGGGTGAATTACGTGATCTGGAAACCATTCGGCTTG
CACTGACTGCGGCTGAAACAGGCCATTTAGTGTTTGGTACACTACACACCTCATCAGCGGCAAAAACTATCGACCGTATT
ATTGACGTATTTCCGGCAGCAGAAAAAGATATGGTGCGGTCAATGTTGTCAGAATCATTACGTGCAGTTATTTCTCAAAC
GCTGCTAAAGAAAGTGGGCGGAGGCCGAATTGCTGCACATGAGATCATGATTGGTACTCCGGCAATCCGCAATTTGATTC
GTGAAGATAAAGTGCCGCAGATGTATTCTTCAATTCAAACGGGTGGTGCTTTGGGCATGCAAACATTAGATCAATGTTTA
CAAGACCTTTTGCGACGCCAGCAAATAACCAAACATGAAGCACTGCCTCGTGCTGTGAACAAGGACCTGTTCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.886

98.841

0.78
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baumannii strain A118

77.907

99.71

0.777
  pilT Acinetobacter nosocomialis M2

77.907

99.71

0.777
  pilT Acinetobacter baumannii D1279779

77.907

99.71

0.777
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.772

96.522

0.693
  pilT Vibrio cholerae strain A1552

71.772

96.522

0.693
  pilT Neisseria meningitidis 8013

69.679

99.42

0.693
  pilT Neisseria gonorrhoeae MS11

69.388

99.42

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.504
  pilU Pseudomonas stutzeri DSM 10701

41.329

100

0.414
  pilU Acinetobacter baylyi ADP1

41.176

98.551

0.406
  pilU Vibrio cholerae strain A1552

40.708

98.261

0.4