Detailed information NTG0677787

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LGT42_RS03565
• Genome accession: NZ_CP095733
• Coordinates: 739783..740925 (+)
• Length: 380 a.a.
• NCBI ID: WP_226071597.1
• Uniprot ID: -
• Organism: Methylophaga pinxianii strain TMB1265
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 734783..745925

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LGT42_RS03540 (LGT42_003540)	-	735323..736471 (-)	1149	WP_226071543.1	homoserine O-acetyltransferase	-
LGT42_RS03545 (LGT42_003545)	-	736496..737071 (-)	576	WP_226071544.1	YggT family protein	-
LGT42_RS03550 (LGT42_003550)	proC	737064..737900 (-)	837	WP_226071548.1	pyrroline-5-carboxylate reductase	-
LGT42_RS03555 (LGT42_003555)	-	737947..738645 (-)	699	WP_226071549.1	YggS family pyridoxal phosphate-dependent enzyme	-
LGT42_RS03560 (LGT42_003560)	pilT	738736..739773 (+)	1038	WP_226071550.1	type IV pilus twitching motility protein PilT	Machinery gene
LGT42_RS03565 (LGT42_003565)	pilU	739783..740925 (+)	1143	WP_226071597.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LGT42_RS03570 (LGT42_003570)	-	741099..741950 (+)	852	WP_226071551.1	OmpA family protein	-
LGT42_RS03575 (LGT42_003575)	-	742346..742540 (+)	195	WP_349630199.1	CsbD family protein	-
LGT42_RS03580 (LGT42_003580)	-	742801..743364 (+)	564	WP_226071552.1	BON domain-containing protein	-
LGT42_RS03585 (LGT42_003585)	-	743372..743833 (+)	462	WP_226071553.1	hypothetical protein	-
LGT42_RS03590 (LGT42_003590)	-	744056..745306 (+)	1251	WP_247363602.1	sigma-54 dependent transcriptional regulator	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42174.24 Da
• Isoelectric Point: 5.3591

>NTDB_id=677787 LGT42_RS03565 WP_226071597.1 739783..740925(+) (pilU) [Methylophaga pinxianii strain TMB1265]
MEIVTILKAAVKHEASDIFITSGRPATLKVNGRMATLSQEPLSEEEARELVLSTMNDEQKRTFERDKECNYAIDTKGAGR
FRVSALYQRSRIAMVLRLVKGDIPTIEELHVPEIIKELAMTKRGLVMFVGATGSGKSTTLAAMIGYRNQNSTGHILTIED
PIEFDHKHAGCVVTQREVGVDTDSYEIALKNSLRQAPDVIMIGEIRSRDTMDYGISFAETGHLCLSTLHANNANQAMERV
INFFPEDRHQQLFLDLSLNLKAVVAQRLIPKKDGSGRAVAVEVLLNTPLVGDLIEKGKVAEIKDVMKRSRELGMQTFDQS
LYDLYKAGEISYEDALRNADSSNEVRLMIKLGSETGELPVEAGAQALSLEMENEPMGTRF

Nucleotide

• Length: 1143 bp

>NTDB_id=677787 LGT42_RS03565 WP_226071597.1 739783..740925(+) (pilU) [Methylophaga pinxianii strain TMB1265]
ATGGAAATTGTCACTATATTAAAAGCGGCGGTAAAACACGAAGCTTCGGATATTTTTATTACCTCTGGTCGACCAGCCAC
TTTAAAAGTAAACGGTCGAATGGCAACACTGTCTCAGGAACCCTTATCTGAAGAAGAGGCTCGAGAATTAGTGCTGAGCA
CTATGAACGATGAGCAAAAACGTACGTTTGAGCGGGATAAGGAGTGTAATTACGCAATTGATACCAAAGGTGCAGGACGC
TTCAGGGTCAGTGCGCTTTATCAGCGAAGCCGAATTGCCATGGTCTTGCGTTTGGTCAAAGGTGATATCCCGACCATTGA
AGAATTGCATGTTCCTGAGATTATCAAAGAGTTGGCCATGACCAAACGTGGCCTGGTGATGTTTGTGGGCGCAACTGGGA
GTGGTAAATCAACGACGTTGGCGGCTATGATTGGTTATCGTAATCAAAACAGCACAGGCCATATACTTACCATTGAAGAC
CCCATTGAGTTTGATCATAAACATGCAGGTTGTGTTGTAACGCAACGTGAAGTTGGAGTGGATACCGACTCATATGAAAT
TGCATTAAAAAATTCATTGCGGCAAGCCCCCGATGTCATTATGATCGGTGAAATCCGGAGTAGGGATACAATGGATTACG
GTATTTCATTTGCTGAAACCGGTCATTTATGTCTTTCAACTCTCCATGCAAATAATGCGAATCAGGCTATGGAGCGGGTC
ATCAACTTTTTCCCGGAAGATAGACATCAGCAATTGTTTTTGGATTTATCGCTGAATCTCAAAGCAGTTGTAGCGCAAAG
ATTAATTCCCAAAAAAGATGGATCTGGTCGCGCGGTAGCGGTTGAAGTTTTACTTAATACGCCACTGGTTGGCGATCTCA
TCGAAAAGGGTAAAGTCGCTGAGATAAAGGATGTCATGAAACGTTCTCGTGAACTTGGAATGCAGACCTTTGATCAGTCG
TTGTATGATTTGTACAAAGCCGGTGAAATCTCCTATGAAGACGCGTTACGAAATGCCGATTCTAGCAATGAAGTGAGATT
GATGATTAAGTTAGGTTCAGAAACTGGTGAACTCCCAGTCGAAGCCGGTGCCCAAGCGTTATCCCTCGAAATGGAAAATG
AACCCATGGGTACTCGTTTTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (6-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.579	100	0.616
pilU	Acinetobacter baylyi ADP1	57.821	94.211	0.545
pilU	Vibrio cholerae strain A1552	55.429	92.105	0.511
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.151	90.526	0.382