Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MW405_RS15290 Genome accession   NZ_CP095518
Coordinates   3205034..3206014 (+) Length   326 a.a.
NCBI ID   WP_001467825.1    Uniprot ID   -
Organism   Escherichia coli strain GN02350     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3200034..3211014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW405_RS15260 (MW405_15260) yggM 3200639..3201646 (+) 1008 WP_000745232.1 DUF1202 family protein -
  MW405_RS15265 (MW405_15265) hemW 3201711..3202847 (-) 1137 WP_000239951.1 radical SAM family heme chaperone HemW -
  MW405_RS15270 (MW405_15270) rdgB 3202840..3203433 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  MW405_RS15275 (MW405_15275) yggU 3203441..3203731 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  MW405_RS15280 (MW405_15280) yggT 3203728..3204294 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  MW405_RS15285 (MW405_15285) yggS 3204312..3205016 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  MW405_RS15290 (MW405_15290) pilT 3205034..3206014 (+) 981 WP_001467825.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MW405_RS15295 (MW405_15295) ruvX 3206188..3206604 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  MW405_RS15300 (MW405_15300) yqgE 3206604..3207167 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  MW405_RS15305 (MW405_15305) gshB 3207276..3208226 (-) 951 WP_000593256.1 glutathione synthase -
  MW405_RS15310 (MW405_15310) rsmE 3208239..3208970 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MW405_RS15315 (MW405_15315) endA 3209050..3209757 (-) 708 WP_001305312.1 deoxyribonuclease I -
  MW405_RS15320 (MW405_15320) yggI 3209852..3210349 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36073.30 Da        Isoelectric Point: 5.9975

>NTDB_id=677311 MW405_RS15290 WP_001467825.1 3205034..3206014(+) (pilT) [Escherichia coli strain GN02350]
MNMEEIVTLSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=677311 MW405_RS15290 WP_001467825.1 3205034..3206014(+) (pilT) [Escherichia coli strain GN02350]
ATGAATATGGAAGAAATTGTGACCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362