Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MWN52_RS14325 Genome accession   NZ_CP095475
Coordinates   3257240..3258277 (-) Length   345 a.a.
NCBI ID   WP_130517185.1    Uniprot ID   -
Organism   Pseudoxanthomonas winnipegensis strain ZSY-33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3252240..3263277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWN52_RS14305 (MWN52_14320) - 3253389..3253958 (+) 570 WP_286575160.1 DNA-3-methyladenine glycosylase I -
  MWN52_RS14310 (MWN52_14325) - 3254517..3255212 (-) 696 WP_337053951.1 YitT family protein -
  MWN52_RS14315 (MWN52_14330) - 3255350..3255919 (-) 570 WP_286575161.1 hypothetical protein -
  MWN52_RS14320 (MWN52_14335) pilU 3255982..3257112 (-) 1131 WP_130517187.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MWN52_RS14325 (MWN52_14340) pilT 3257240..3258277 (-) 1038 WP_130517185.1 type IV pilus twitching motility protein PilT Machinery gene
  MWN52_RS14330 (MWN52_14345) - 3258406..3259059 (+) 654 WP_286577751.1 YggS family pyridoxal phosphate-dependent enzyme -
  MWN52_RS14335 (MWN52_14350) proC 3259519..3260370 (+) 852 WP_286575165.1 pyrroline-5-carboxylate reductase -
  MWN52_RS14340 (MWN52_14355) - 3260376..3260846 (+) 471 WP_286575166.1 DUF4426 domain-containing protein -
  MWN52_RS14345 (MWN52_14360) soxR 3260866..3261300 (-) 435 WP_130517177.1 redox-sensitive transcriptional activator SoxR -
  MWN52_RS14350 (MWN52_14365) - 3261396..3262763 (+) 1368 WP_286575167.1 MFS transporter -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38709.43 Da        Isoelectric Point: 6.7269

>NTDB_id=677124 MWN52_RS14325 WP_130517185.1 3257240..3258277(-) (pilT) [Pseudoxanthomonas winnipegensis strain ZSY-33]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREMVQQPQGLILVTGPTGSGKSTTLAAMIDYINKSEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSQITRHQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=677124 MWN52_RS14325 WP_130517185.1 3257240..3258277(-) (pilT) [Pseudoxanthomonas winnipegensis strain ZSY-33]
ATGGATATCGCCGAACTACTGGCCTTCTCGGTCAAGAACAAGGCCTCGGACCTGCATCTGTCCGCCGGGCTGCCGCCGAT
GATCCGCGTGGATGGCGACGTGCGCCGCATCAACATCCCCGCCCTGGACCACAAGCAGGTCCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCCTTCGAGATCCCCTCGCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATCCCGTCCGAGGTGCTGACCCTGGA
GGACCTGGCCTGTCCGCCGCTGTTCCGCGAGATGGTGCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGACCGACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCGATGATCGACTACATCAACAAGAGCGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGA
AGCGCTGCGCTCGGCGCTGCGCGAGGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCATCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTAATCAGCCAGGC
GCTGCTGAAGAAGGTCGGCGGTGGCCGCACCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCAGATCACGCGCCATCAGGCCAAGGAATACGCCAAGGACAAGCGGTTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.103

98.841

0.643
  pilT Neisseria gonorrhoeae MS11

64.809

98.841

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.118

98.261

0.394
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391