Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MWN52_RS14320 Genome accession   NZ_CP095475
Coordinates   3255982..3257112 (-) Length   376 a.a.
NCBI ID   WP_130517187.1    Uniprot ID   A0A4Q9TBT5
Organism   Pseudoxanthomonas winnipegensis strain ZSY-33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3250982..3262112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWN52_RS14300 (MWN52_14315) recQ 3251553..3253352 (+) 1800 WP_286575159.1 DNA helicase RecQ -
  MWN52_RS14305 (MWN52_14320) - 3253389..3253958 (+) 570 WP_286575160.1 DNA-3-methyladenine glycosylase I -
  MWN52_RS14310 (MWN52_14325) - 3254517..3255212 (-) 696 WP_337053951.1 YitT family protein -
  MWN52_RS14315 (MWN52_14330) - 3255350..3255919 (-) 570 WP_286575161.1 hypothetical protein -
  MWN52_RS14320 (MWN52_14335) pilU 3255982..3257112 (-) 1131 WP_130517187.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MWN52_RS14325 (MWN52_14340) pilT 3257240..3258277 (-) 1038 WP_130517185.1 type IV pilus twitching motility protein PilT Machinery gene
  MWN52_RS14330 (MWN52_14345) - 3258406..3259059 (+) 654 WP_286577751.1 YggS family pyridoxal phosphate-dependent enzyme -
  MWN52_RS14335 (MWN52_14350) proC 3259519..3260370 (+) 852 WP_286575165.1 pyrroline-5-carboxylate reductase -
  MWN52_RS14340 (MWN52_14355) - 3260376..3260846 (+) 471 WP_286575166.1 DUF4426 domain-containing protein -
  MWN52_RS14345 (MWN52_14360) soxR 3260866..3261300 (-) 435 WP_130517177.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41848.14 Da        Isoelectric Point: 6.8286

>NTDB_id=677123 MWN52_RS14320 WP_130517187.1 3255982..3257112(-) (pilU) [Pseudoxanthomonas winnipegensis strain ZSY-33]
MSAIDFTSFLKLMAHQKASDLFITSGMPPSIKVHGKLTPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETKIPSVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLVPTPDGKARRVAMEIMLGTPLIQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLAQGLDGVEVAEAR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=677123 MWN52_RS14320 WP_130517187.1 3255982..3257112(-) (pilU) [Pseudoxanthomonas winnipegensis strain ZSY-33]
ATGAGCGCCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGTCGATCAAGGTGCACGGCAAGCTCACTCCGATCACGCAGACGCCGCTGACGCCGCAGCAGTCGCGCGACCTGG
TGCTCAACGTCATGACCCCCGCCCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGC
GTGGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCC
CAGCGTCGATGAGCTCAACCTGCCGCCGGTCATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGTCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGTGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCTCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTCTCGCTCAACCTCAAGGGCGTGGT
CGCCCAGCAGCTGGTCCCGACCCCCGACGGCAAGGCGCGCCGGGTGGCGATGGAGATCATGCTGGGCACGCCGCTGATCC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCTCCTACGAGGACGCCCTGCGCTACGCCGATTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCGCGCACCCTGGCCCAGGGCCTGGACGGCGTCGAGGTCGCCG
AGGCGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Q9TBT5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.429

93.085

0.386
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Legionella pneumophila strain Lp02

40.896

89.096

0.364
  pilT Legionella pneumophila strain ERS1305867

40.896

89.096

0.364
  pilT Pseudomonas aeruginosa PAK

40.597

89.096

0.362