Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CB4_RS05760 Genome accession   NZ_AP017312
Coordinates   1132803..1133846 (+) Length   347 a.a.
NCBI ID   WP_096463976.1    Uniprot ID   A0A0U5AT79
Organism   Aneurinibacillus soli strain CB4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1127803..1138846
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CB4_RS05740 (CB4_01151) - 1128219..1128686 (-) 468 WP_157737813.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CB4_RS05745 (CB4_01152) - 1128817..1129530 (+) 714 WP_231956157.1 hypothetical protein -
  CB4_RS05750 (CB4_01153) - 1129523..1131058 (+) 1536 WP_172890807.1 VanW family protein -
  CB4_RS05755 (CB4_01154) - 1131079..1132737 (+) 1659 WP_096463975.1 GspE/PulE family protein -
  CB4_RS05760 (CB4_01155) pilT 1132803..1133846 (+) 1044 WP_096463976.1 type IV pilus twitching motility protein PilT Machinery gene
  CB4_RS05765 (CB4_01156) - 1133827..1135062 (+) 1236 WP_331713185.1 type II secretion system F family protein -
  CB4_RS05770 (CB4_01157) - 1135084..1135527 (+) 444 WP_096463977.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CB4_RS05775 (CB4_01158) - 1135715..1136485 (+) 771 WP_231956158.1 A24 family peptidase -
  CB4_RS05780 (CB4_01159) pilM 1136536..1137537 (+) 1002 WP_096463978.1 type IV pilus biogenesis protein PilM -
  CB4_RS05785 (CB4_01160) - 1137543..1138154 (+) 612 WP_096463980.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38283.23 Da        Isoelectric Point: 7.5674

>NTDB_id=67668 CB4_RS05760 WP_096463976.1 1132803..1133846(+) (pilT) [Aneurinibacillus soli strain CB4]
MSEIDIKKLLKYACEKKASDLHITVGSAPVFRIDGELRRLDIPSLTPQDTELMARELIRENLYASFIERGELDFSYGLPG
VSRFRVNAYHQRGCISLVARVVPSGIPGLDSLALPEVLKTLCRKPQGLVLVTGPTGSGKSTTLAAMIDYINSTMRKHIIT
LEDPIEYLHKHQLSIINQREVGFDTNNFASGLRSALRQDPDVILVGEMRDLETISTAITAAETGHLVFATLHTSDAPQTI
DRIIDVFPGSQQPQVRIQLASVLVSIVSQRLFPKVGGGRVAATEVLVNTSAIGNLIRMEKVHQIKSMMQTGRELGMHTME
MSIKELLGQGSVARQAVQHHLNERAFE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=67668 CB4_RS05760 WP_096463976.1 1132803..1133846(+) (pilT) [Aneurinibacillus soli strain CB4]
ATGAGCGAGATTGATATTAAGAAATTGCTAAAGTACGCTTGTGAGAAGAAAGCGTCTGATCTTCATATCACGGTGGGATC
TGCTCCCGTTTTCCGGATTGATGGGGAATTAAGACGGCTTGATATTCCGTCGCTTACACCGCAGGATACGGAACTGATGG
CCCGCGAGCTAATTCGAGAGAATCTGTACGCGTCGTTTATCGAACGGGGGGAGCTTGATTTCTCATACGGCCTGCCGGGA
GTATCGCGTTTTCGTGTTAATGCGTATCATCAGCGCGGCTGTATCAGTCTGGTAGCTCGCGTTGTGCCATCGGGTATTCC
AGGACTTGATTCTCTGGCGTTGCCTGAAGTACTCAAGACGCTTTGTCGCAAACCACAGGGGCTTGTACTCGTTACAGGTC
CGACGGGTAGCGGGAAATCGACCACGCTTGCGGCAATGATCGACTATATTAACAGTACGATGCGCAAGCATATCATTACG
CTGGAAGATCCGATTGAATATTTGCATAAGCATCAGCTTTCAATCATCAACCAGCGGGAGGTCGGATTTGACACGAATAA
TTTTGCGAGCGGGCTTCGATCAGCGCTCAGACAGGACCCGGATGTCATTCTAGTTGGGGAGATGCGTGATCTGGAGACGA
TTAGTACGGCGATTACGGCAGCGGAAACCGGGCACCTCGTATTCGCAACGTTGCACACATCGGATGCACCGCAGACCATT
GATCGCATCATTGATGTATTTCCAGGGAGTCAGCAGCCGCAGGTTCGAATTCAACTGGCATCTGTTCTGGTCAGCATTGT
CTCTCAGCGTTTGTTCCCAAAAGTGGGGGGCGGACGGGTAGCAGCGACAGAAGTGCTGGTGAATACATCGGCCATCGGCA
ACTTGATTCGCATGGAGAAAGTGCATCAGATTAAAAGCATGATGCAGACAGGTCGCGAGCTTGGCATGCACACGATGGAG
ATGTCGATTAAGGAACTGCTGGGTCAGGGGAGCGTCGCACGTCAGGCGGTACAGCACCATCTGAATGAAAGGGCTTTTGA
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U5AT79

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

53.468

99.712

0.533
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.468

99.712

0.533
  pilT Pseudomonas aeruginosa PAK

50.904

95.677

0.487
  pilT Pseudomonas stutzeri DSM 10701

49.851

96.542

0.481
  pilT Acinetobacter baumannii D1279779

49.851

96.542

0.481
  pilT Acinetobacter nosocomialis M2

49.851

96.542

0.481
  pilT Acinetobacter baumannii strain A118

49.851

96.542

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.765

94.236

0.478
  pilT Acinetobacter baylyi ADP1

50.459

94.236

0.476
  pilT Legionella pneumophila strain Lp02

47.965

99.135

0.476
  pilT Legionella pneumophila strain ERS1305867

47.965

99.135

0.476
  pilT Neisseria gonorrhoeae MS11

47.619

96.83

0.461
  pilT Neisseria meningitidis 8013

48.338

95.389

0.461
  pilU Pseudomonas stutzeri DSM 10701

42.604

97.406

0.415
  pilU Acinetobacter baylyi ADP1

40.597

96.542

0.392
  pilU Vibrio cholerae strain A1552

40.059

97.118

0.389

Multiple sequence alignment