Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MRK30_RS14640 Genome accession   NZ_CP095357
Coordinates   2983090..2984127 (+) Length   345 a.a.
NCBI ID   WP_002121749.1    Uniprot ID   A0A009I411
Organism   Acinetobacter pittii strain 19MO01SH04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2978090..2989127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK30_RS14630 (MRK30_14665) sbcC 2978474..2982070 (+) 3597 WP_032057668.1 AAA family ATPase -
  MRK30_RS14635 (MRK30_14670) yggS 2982271..2982963 (-) 693 WP_032057669.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRK30_RS14640 (MRK30_14675) pilT 2983090..2984127 (+) 1038 WP_002121749.1 type IV pilus twitching motility protein PilT Machinery gene
  MRK30_RS14645 (MRK30_14680) pilU 2984147..2985265 (+) 1119 WP_032057670.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MRK30_RS14650 (MRK30_14685) fur 2985335..2985772 (-) 438 WP_002121758.1 ferric iron uptake transcriptional regulator -
  MRK30_RS14655 (MRK30_14690) omlA 2985884..2986282 (+) 399 WP_002121740.1 outer membrane protein assembly factor BamE -
  MRK30_RS14660 (MRK30_14695) - 2986347..2986661 (-) 315 WP_017481335.1 RnfH family protein -
  MRK30_RS14665 (MRK30_14700) - 2986661..2987728 (-) 1068 WP_031944146.1 membrane protein -
  MRK30_RS14670 (MRK30_14705) - 2987882..2988337 (-) 456 WP_002121698.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38389.97 Da        Isoelectric Point: 6.7269

>NTDB_id=676488 MRK30_RS14640 WP_002121749.1 2983090..2984127(+) (pilT) [Acinetobacter pittii strain 19MO01SH04]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALDHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KNLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=676488 MRK30_RS14640 WP_002121749.1 2983090..2984127(+) (pilT) [Acinetobacter pittii strain 19MO01SH04]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACACCTATCTGCTGGCATGCCACCAAT
GATTCGCGTTGATGGTGAAGTCCGTCGCATTAACTTACCTGCTCTAGATCATAAAGATGTTCATCGTTTGGTCTACGACA
TCATGAATGATAAACAGCGTCGTGACTACGAAGAAAAACTCGAAACCGACTTTTCTTTTGAAGTGCCCAACGTTGCTCGT
TTCCGTGTAAACGCATTTAACCAAAACCGTGGTGCGGGTGCAGTGTTTCGTACCATTCCATCCAAAGTACTCACCATGGA
AGATTTAGGCTTAGGACAAATCTTTAAAGATATTTGTGACTATCCTCGCGGGATTGTACTGGTGACAGGCCCAACAGGTT
CTGGTAAATCGACCACACTTGCAGCGATGATGGATTATATCAACGAAAACCGTTATGACCATATTTTAACGGTTGAAGAC
CCTATCGAATTTGTACACCAGTCTAAGAAATGTCTCATCAATCAACGTGAAGTGCATCGTGATACACATGGCTTTAATGA
GGCGCTACGTTCGGCACTGCGTGAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAGACTATTCGTTTAG
CACTTACTGCTGCCGAAACAGGTCATTTGGTGTTTGGTACGCTTCATACCACTTCTGCTGCAAAAACGATTGACCGTGTA
ATTGACGTATTCCCAGCCGAAGAAAAAGATATGGTTCGTGCCATGCTCTCTGAATCATTGCAGGCTGTTATTTCACAAAC
CTTGCTTAAAAAGAATGGCGGCGGTCGTGTCGCCGCTCATGAAATCATGATTGGTATTCCTGCGATTCGTAACTTAATTC
GTGAAAACAAAGTCGCGCAAATGTACTCAGCTATTCAGACTGGCGCAAATCATGGCATGACCACCCTTGACCAAAGCTTA
AAGAATTTGGTGTCTCGCGGAGTGATCAGCCCGCAAACGGCTCGTACTGCTGCTAAACAGCCTGAATCATTTCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009I411

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.13

100

0.991
  pilT Acinetobacter baumannii D1279779

98.841

100

0.988
  pilT Acinetobacter baumannii strain A118

98.841

100

0.988
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391