Detailed information NTG0676489

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MRK30_RS14645
• Genome accession: NZ_CP095357
• Coordinates: 2984147..2985265 (+)
• Length: 372 a.a.
• NCBI ID: WP_032057670.1
• Uniprot ID: -
• Organism: Acinetobacter pittii strain 19MO01SH04
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2979147..2990265

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MRK30_RS14635 (MRK30_14670)	yggS	2982271..2982963 (-)	693	WP_032057669.1	YggS family pyridoxal phosphate-dependent enzyme	-
MRK30_RS14640 (MRK30_14675)	pilT	2983090..2984127 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
MRK30_RS14645 (MRK30_14680)	pilU	2984147..2985265 (+)	1119	WP_032057670.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MRK30_RS14650 (MRK30_14685)	fur	2985335..2985772 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
MRK30_RS14655 (MRK30_14690)	omlA	2985884..2986282 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
MRK30_RS14660 (MRK30_14695)	-	2986347..2986661 (-)	315	WP_017481335.1	RnfH family protein	-
MRK30_RS14665 (MRK30_14700)	-	2986661..2987728 (-)	1068	WP_031944146.1	membrane protein	-
MRK30_RS14670 (MRK30_14705)	-	2987882..2988337 (-)	456	WP_002121698.1	bacteriohemerythrin	-
MRK30_RS14675 (MRK30_14710)	-	2988589..2989437 (-)	849	WP_014205980.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41975.16 Da
• Isoelectric Point: 6.5062

>NTDB_id=676489 MRK30_RS14645 WP_032057670.1 2984147..2985265(+) (pilU) [Acinetobacter pittii strain 19MO01SH04]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEDPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=676489 MRK30_RS14645 WP_032057670.1 2984147..2985265(+) (pilU) [Acinetobacter pittii strain 19MO01SH04]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAATTTTGCGATTATGAACCGCGATAAAAGTGCG
CGCTTTCGTGTCAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTATTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAATTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCTTTGGCTTCAATGATTAGCTATCGGAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGTTGCATCATTACCCAACGTGAAGTCGGGATTGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCTCGCCGTGCAGCGATTGAGATTCTCATTAACTCCCCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTCGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGATCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.003	99.731	0.688
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363