Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MRO11_RS02170 Genome accession   NZ_CP095336
Coordinates   482885..483922 (+) Length   345 a.a.
NCBI ID   WP_154169236.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 19GA03SH07     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477885..488922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRO11_RS02140 (MRO11_02140) hemW 478477..479646 (-) 1170 WP_354672547.1 radical SAM family heme chaperone HemW -
  MRO11_RS02145 (MRO11_02145) - 479640..480245 (-) 606 WP_161439137.1 XTP/dITP diphosphatase -
  MRO11_RS02150 (MRO11_02150) yggU 480338..480631 (-) 294 WP_004396060.1 DUF167 family protein YggU -
  MRO11_RS02155 (MRO11_02155) - 480631..481188 (-) 558 WP_040904707.1 YggT family protein -
  MRO11_RS02160 (MRO11_02160) proC 481241..482059 (-) 819 WP_332405324.1 pyrroline-5-carboxylate reductase -
  MRO11_RS02165 (MRO11_02165) - 482142..482858 (-) 717 WP_154169238.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRO11_RS02170 (MRO11_02170) pilT 482885..483922 (+) 1038 WP_154169236.1 type IV pilus twitching motility protein PilT Machinery gene
  MRO11_RS02175 (MRO11_02175) pilU 483933..485039 (+) 1107 WP_187985462.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MRO11_RS02180 (MRO11_02180) ruvX 485042..485467 (-) 426 WP_187985463.1 Holliday junction resolvase RuvX -
  MRO11_RS02185 (MRO11_02185) - 485528..486091 (-) 564 WP_040904706.1 YqgE/AlgH family protein -
  MRO11_RS02190 (MRO11_02190) gshB 486131..487087 (-) 957 WP_158137782.1 glutathione synthase -
  MRO11_RS02195 (MRO11_02195) rsmE 487102..487833 (-) 732 WP_154169232.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MRO11_RS02200 (MRO11_02200) - 488134..488847 (-) 714 WP_161439132.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38232.67 Da        Isoelectric Point: 6.8231

>NTDB_id=676419 MRO11_RS02170 WP_154169236.1 482885..483922(+) (pilT) [Vibrio metschnikovii strain 19GA03SH07]
MDISELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFSHADVHRLIFAIMNDAQRSEYEERLEVDFSFELPNVGR
FRVNAFHQSRGSSAVFRTIPTVIPTLDQLNAPEIFRKISNYEKGLVLVTGPTGSGKSTTLAAMVDYINTHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLFKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQHA
KQLVAQGVVDLPEVQQKLGLDSSSF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=676419 MRO11_RS02170 WP_154169236.1 482885..483922(+) (pilT) [Vibrio metschnikovii strain 19GA03SH07]
ATGGATATCTCAGAGTTACTGGATTTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCAGGTGTTCCGCCCAT
GGTCCGGATTGATGGTGAAGTCCGTAAGCTGGGAGTGCCTGCTTTTAGTCATGCTGACGTGCATCGATTAATCTTTGCCA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGCGCCTCGAAGTCGATTTTTCCTTTGAGCTACCGAATGTGGGACGT
TTTCGGGTTAATGCCTTTCATCAGTCGCGCGGCAGCTCCGCCGTATTTCGAACGATTCCTACAGTGATCCCAACGTTGGA
TCAGTTGAATGCTCCGGAGATCTTTCGAAAAATATCCAATTATGAGAAAGGGTTAGTCCTTGTTACTGGCCCCACTGGAT
CCGGTAAATCGACAACGTTAGCGGCAATGGTTGATTATATCAATACTCATCATAATAAACACATTTTGACCATTGAAGAT
CCGATAGAATTTGTGCATAGCAATAATAAGTGTTTGATTAACCAGCGTGAAGTTCATCGTGATACACATAGTTTTAAAAA
TGCGTTACGTTCGGCGCTGCGAGAAGACCCTGATGTGATCTTAGTCGGTGAGCTGCGTGATCAAGAGACAATCAGTTTAG
CGTTAACCGCCGCTGAAACGGGACATTTAGTGTTTGGTACGCTACACACTAGTTCTGCCGCAAAAACCATTGATCGGATT
ATCGATGTATTCCCGGGCAGCGATAAAGACATGGTGCGTTCGATGTTATCGGAATCGCTGCGTGCGGTGATTGCGCAAAA
ACTGTTTAAGCGAGTCGGAGGGGGGCGCGTGGCGTGTCACGAAATTATGTTAGCGACTCCTGCGATTCGTAACTTGATCC
GGGAAGATAAAGTCGCACAGATGTATTCGATCATCCAAACGGGCGCTGCGCATGGGATGCAAACTATGGAGCAGCATGCT
AAGCAGTTGGTCGCTCAAGGGGTTGTGGATTTACCAGAGGTCCAACAAAAACTTGGTTTAGACAGCTCATCATTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.174

100

0.922
  pilT Vibrio cholerae strain A1552

92.174

100

0.922
  pilT Acinetobacter baumannii strain A118

70.145

100

0.701
  pilT Acinetobacter baumannii D1279779

70.145

100

0.701
  pilT Acinetobacter nosocomialis M2

69.855

100

0.699
  pilT Acinetobacter baylyi ADP1

72.289

96.232

0.696
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

67.953

97.681

0.664
  pilT Legionella pneumophila strain Lp02

67.781

95.362

0.646
  pilT Legionella pneumophila strain ERS1305867

67.781

95.362

0.646
  pilT Neisseria meningitidis 8013

63.006

100

0.632
  pilT Neisseria gonorrhoeae MS11

62.428

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.16

93.913

0.49
  pilU Vibrio cholerae strain A1552

43.692

94.203

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.606

95.652

0.388
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377