Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MRO11_RS02175 Genome accession   NZ_CP095336
Coordinates   483933..485039 (+) Length   368 a.a.
NCBI ID   WP_187985462.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 19GA03SH07     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478933..490039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRO11_RS02145 (MRO11_02145) - 479640..480245 (-) 606 WP_161439137.1 XTP/dITP diphosphatase -
  MRO11_RS02150 (MRO11_02150) yggU 480338..480631 (-) 294 WP_004396060.1 DUF167 family protein YggU -
  MRO11_RS02155 (MRO11_02155) - 480631..481188 (-) 558 WP_040904707.1 YggT family protein -
  MRO11_RS02160 (MRO11_02160) proC 481241..482059 (-) 819 WP_332405324.1 pyrroline-5-carboxylate reductase -
  MRO11_RS02165 (MRO11_02165) - 482142..482858 (-) 717 WP_154169238.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRO11_RS02170 (MRO11_02170) pilT 482885..483922 (+) 1038 WP_154169236.1 type IV pilus twitching motility protein PilT Machinery gene
  MRO11_RS02175 (MRO11_02175) pilU 483933..485039 (+) 1107 WP_187985462.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MRO11_RS02180 (MRO11_02180) ruvX 485042..485467 (-) 426 WP_187985463.1 Holliday junction resolvase RuvX -
  MRO11_RS02185 (MRO11_02185) - 485528..486091 (-) 564 WP_040904706.1 YqgE/AlgH family protein -
  MRO11_RS02190 (MRO11_02190) gshB 486131..487087 (-) 957 WP_158137782.1 glutathione synthase -
  MRO11_RS02195 (MRO11_02195) rsmE 487102..487833 (-) 732 WP_154169232.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MRO11_RS02200 (MRO11_02200) - 488134..488847 (-) 714 WP_161439132.1 endonuclease -
  MRO11_RS02205 (MRO11_02205) - 488933..489421 (-) 489 WP_332405326.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41432.56 Da        Isoelectric Point: 5.8645

>NTDB_id=676420 MRO11_RS02175 WP_187985462.1 483933..485039(+) (pilU) [Vibrio metschnikovii strain 19GA03SH07]
MQLDQILQEMLTQGASDLYITVAAPILYRVNGDLQPQGEALNEAQVKELLLRMMDSDRQTEFNQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETQIPSFDDLQLPEVLKDLALTKRGLVLVVGATGSGKSTTMAAMTGYRNLKTRGHILTVEDPI
EFVHPHQGCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LMPKEKKEQFLFDLSMNLKGVIGQQLLRDKQGKGRHGVFEILLNSPRMADLIRRGDLHELKATMAKSKEVGMQTFDQALY
QLVIDDKITEQDALHSADSANDLRLMLKTQRGEGYSSGALDNIKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=676420 MRO11_RS02175 WP_187985462.1 483933..485039(+) (pilU) [Vibrio metschnikovii strain 19GA03SH07]
ATGCAATTGGATCAAATATTACAAGAGATGCTCACCCAAGGGGCGTCCGATCTCTACATCACTGTCGCGGCTCCAATTTT
GTATCGCGTTAATGGCGATCTCCAACCTCAGGGGGAAGCGTTAAATGAAGCTCAGGTCAAAGAGTTGTTATTGCGGATGA
TGGATAGCGACCGACAAACGGAGTTTAACCAAACGCGTGAGGCCAATTTTGCGATTGTGCGTGATTTTGGCCGTTTCCGA
GTCAGTGCTTTTTTTCAGCGTGAGCTACCGGGTGCCGTTATTCGCCGGATCGAAACGCAAATTCCTAGTTTTGATGACTT
GCAGTTGCCGGAGGTGCTTAAAGATCTCGCGCTAACCAAGCGGGGTTTAGTGTTAGTGGTAGGTGCGACAGGCTCGGGAA
AATCAACCACCATGGCGGCGATGACCGGTTATCGTAATTTGAAAACCCGCGGGCACATTCTTACTGTCGAGGATCCGATT
GAATTTGTTCATCCTCATCAAGGCTGTATTGTGACTCAGCGTGAAGTCGGCTTAGATACTGAGAGCTATGAAGTGGCATT
GAAAAATTCACTTCGCCAAGCGCCGGACATGATTTTGATTGGTGAAATACGCAGTCGTGAAACCATGGAATACGCGATGA
CCTTTGCCGAAACCGGGCATTTATGTATGGCAACCTTACACGCCAACAATGCCAACCAAGCCTTAGAGCGTATTTTACAT
TTGATGCCGAAAGAGAAAAAAGAACAGTTTCTGTTTGATTTATCGATGAACTTAAAAGGAGTGATTGGTCAGCAACTGCT
GCGTGATAAACAAGGTAAAGGGCGTCATGGCGTGTTTGAGATTTTGCTCAATAGTCCAAGAATGGCGGATTTGATCCGCC
GTGGCGATTTGCACGAATTAAAAGCAACCATGGCTAAATCGAAAGAAGTGGGAATGCAAACCTTCGATCAAGCGCTGTAT
CAATTGGTGATTGACGATAAAATCACTGAGCAAGATGCGCTGCACAGCGCCGACTCAGCCAATGATTTACGCCTTATGCT
CAAAACCCAGCGGGGAGAGGGCTACTCTTCTGGTGCGTTGGATAATATTAAGATTGATATGGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.152

100

0.832
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

52.991

95.38

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.667

97.826

0.408
  pilT Legionella pneumophila strain ERS1305867

40.413

92.12

0.372
  pilT Legionella pneumophila strain Lp02

40.413

92.12

0.372
  pilT Pseudomonas stutzeri DSM 10701

39.823

92.12

0.367
  pilT Pseudomonas aeruginosa PAK

39.823

92.12

0.367
  pilT Acinetobacter baumannii D1279779

39.528

92.12

0.364
  pilT Acinetobacter baumannii strain A118

39.528

92.12

0.364
  pilT Acinetobacter nosocomialis M2

39.528

92.12

0.364