Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MRK42_RS02975 Genome accession   NZ_CP095328
Coordinates   631415..632449 (+) Length   344 a.a.
NCBI ID   WP_026457847.1    Uniprot ID   A0AAU6TAH8
Organism   Aeromonas sp. 19NY04SH05-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 626415..637449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK42_RS02945 (MRK42_02945) - 627179..628375 (+) 1197 WP_026457680.1 NupC/NupG family nucleoside CNT transporter -
  MRK42_RS02950 (MRK42_02950) - 628481..628900 (-) 420 WP_026457681.1 DUF4426 domain-containing protein -
  MRK42_RS02955 (MRK42_02955) yggU 628937..629230 (-) 294 WP_042026655.1 DUF167 family protein YggU -
  MRK42_RS02960 (MRK42_02960) - 629230..629781 (-) 552 WP_026457844.1 YggT family protein -
  MRK42_RS02965 (MRK42_02965) proC 629793..630617 (-) 825 WP_338611755.1 pyrroline-5-carboxylate reductase -
  MRK42_RS02970 (MRK42_02970) - 630674..631375 (-) 702 WP_338611756.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRK42_RS02975 (MRK42_02975) pilT 631415..632449 (+) 1035 WP_026457847.1 type IVa pilus ATPase TapT Machinery gene
  MRK42_RS02980 (MRK42_02980) pilU 632478..633587 (+) 1110 WP_042026652.1 type IVa pilus ATPase TapU Machinery gene
  MRK42_RS02985 (MRK42_02985) yaaA 633631..634404 (+) 774 WP_042026651.1 peroxide stress protein YaaA -
  MRK42_RS02990 (MRK42_02990) - 634594..635019 (+) 426 WP_042026650.1 hypothetical protein -
  MRK42_RS02995 (MRK42_02995) - 635374..635865 (-) 492 WP_026456193.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  MRK42_RS03000 (MRK42_03000) arfB 635896..636312 (-) 417 WP_026456194.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  MRK42_RS03005 (MRK42_03005) glnK 636514..636852 (+) 339 WP_026456195.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38215.98 Da        Isoelectric Point: 6.7440

>NTDB_id=676350 MRK42_RS02975 WP_026457847.1 631415..632449(+) (pilT) [Aeromonas sp. 19NY04SH05-1]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSAALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSV

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=676350 MRK42_RS02975 WP_026457847.1 631415..632449(+) (pilT) [Aeromonas sp. 19NY04SH05-1]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTGTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGACGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAGCACAGGGAAGTTCACGCCCTCATCTATGACA
TCATGAACGACCACCAGCGCAAGGAGCTGGAGGAAAATTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCGCGT
TTCCGGGTCAACGCCTTTCAGCAGGCGCGTGGATCCGGCGCCGTGTTTCGTACCATCCCCAGTACGGTACTGAGCCTTGA
GGATCTTGATGCCCCCGAGATATTTCGCAAGATTGCCGAATTCCCACGGGGTCTGGTGCTGGTGACGGGCCCGACCGGCT
CGGGCAAATCCACCACTCTGGCGGCCATGGTCAACTACATCAACGAGAACTTTCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGTCTGGTCAACCAGCGGGAAGTGCACCGCGATACCAAGAGTTTCAGCGC
CGCCCTGCGTTCGGCCCTGCGTGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGGGACCTCGAGACCATCCGACTCG
CCATGACCGCGGCCGAGACCGGTCACCTGGTGTTCGGCACCTTGCACACCTCGTCGGCGGCTAAGACCATAGACCGTATC
ATCGACGTCTTCCCCGGCGCGGAGAAGGACATGGTGCGCTCCATGCTGTCCGAATCCCTGCGGGCTGTCATCTCCCAGAC
TCTGCTCAAGCGGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTGCGAAACCTCATTC
GGGAAGACAAGATCGCCCAGCTTTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAACAGCTGGTCAGCCGGGGTGTGGTGGCGTCCCTCGATGCCAAGGCCAAGGCCGTCGATCCCAACAGCGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.551

99.709

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.259

99.709

0.77
  pilT Acinetobacter baumannii strain A118

77.259

99.709

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.385

99.709

0.762
  pilT Pseudomonas aeruginosa PAK

75.51

99.709

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.668

97.965

0.741
  pilT Vibrio cholerae strain A1552

75.668

97.965

0.741
  pilT Legionella pneumophila strain Lp02

73.235

98.837

0.724
  pilT Legionella pneumophila strain ERS1305867

73.235

98.837

0.724
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407