Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MRK42_RS02980 Genome accession   NZ_CP095328
Coordinates   632478..633587 (+) Length   369 a.a.
NCBI ID   WP_042026652.1    Uniprot ID   A0AAU6TAV3
Organism   Aeromonas sp. 19NY04SH05-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 627478..638587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK42_RS02950 (MRK42_02950) - 628481..628900 (-) 420 WP_026457681.1 DUF4426 domain-containing protein -
  MRK42_RS02955 (MRK42_02955) yggU 628937..629230 (-) 294 WP_042026655.1 DUF167 family protein YggU -
  MRK42_RS02960 (MRK42_02960) - 629230..629781 (-) 552 WP_026457844.1 YggT family protein -
  MRK42_RS02965 (MRK42_02965) proC 629793..630617 (-) 825 WP_338611755.1 pyrroline-5-carboxylate reductase -
  MRK42_RS02970 (MRK42_02970) - 630674..631375 (-) 702 WP_338611756.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRK42_RS02975 (MRK42_02975) pilT 631415..632449 (+) 1035 WP_026457847.1 type IVa pilus ATPase TapT Machinery gene
  MRK42_RS02980 (MRK42_02980) pilU 632478..633587 (+) 1110 WP_042026652.1 type IVa pilus ATPase TapU Machinery gene
  MRK42_RS02985 (MRK42_02985) yaaA 633631..634404 (+) 774 WP_042026651.1 peroxide stress protein YaaA -
  MRK42_RS02990 (MRK42_02990) - 634594..635019 (+) 426 WP_042026650.1 hypothetical protein -
  MRK42_RS02995 (MRK42_02995) - 635374..635865 (-) 492 WP_026456193.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  MRK42_RS03000 (MRK42_03000) arfB 635896..636312 (-) 417 WP_026456194.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  MRK42_RS03005 (MRK42_03005) glnK 636514..636852 (+) 339 WP_026456195.1 P-II family nitrogen regulator -
  MRK42_RS03010 (MRK42_03010) - 636993..637463 (-) 471 WP_026456196.1 MarR family transcriptional regulator -
  MRK42_RS03015 (MRK42_03015) - 637623..638150 (+) 528 WP_354688894.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41169.16 Da        Isoelectric Point: 6.2710

>NTDB_id=676351 MRK42_RS02980 WP_042026652.1 632478..633587(+) (pilU) [Aeromonas sp. 19NY04SH05-1]
MNLDQMLAELVSRKGSDLFVTVGSPPTLKVNGHLVALGNEPLDKKEALTLVRETLSSDHFERYIRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDDLSLPPILQDVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRGIVAQQLVPSIDGRRRFAAFEILLNTPLITDIIRKGEMHRLKEVMTKSSELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGHEQLGSGMLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=676351 MRK42_RS02980 WP_042026652.1 632478..633587(+) (pilU) [Aeromonas sp. 19NY04SH05-1]
ATGAATCTGGATCAGATGCTGGCCGAGCTGGTCAGCCGCAAGGGCTCGGATCTGTTTGTGACGGTGGGCTCGCCTCCCAC
CCTCAAGGTCAATGGTCACCTGGTGGCGCTGGGCAACGAGCCCCTCGACAAGAAGGAGGCGCTGACCCTGGTCAGGGAGA
CCCTCAGCTCGGACCATTTTGAACGCTACATCCGAACCAAGGAGGCCAACTACGCCATCCATCGTGAGGAGCTGGGCCGT
TTTCGGGTCAGTGCCTTCTGGCAGCAAGAGCTGCCTGGCATGGTGCTGCGCCGCATCGAGACTCGCATTCCCACCTTCGA
TGATCTGAGCCTGCCTCCCATATTGCAGGACGTGGCGATGGCCAAGCGGGGACTGGTGTTGTTCGTCGGGGCCACGGGGG
CGGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGCGGATGGGCACATACTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCACCAACACGGCCGCAGCCTGGTGACCCAGCGCGAGGTGGGGATAGACACCGAATCGTTCGACGT
GGCGCTGAAGAGCTCTCTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACTGGTCACCTCTGTCTGGCAACTCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAACACCGCCAGTTTCTGTTCGATCTCTCGTTCAACCTGCGTGGCATCGTTGCCCAGCA
ACTGGTGCCCAGTATCGACGGACGGCGACGTTTTGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGAGAGATGCACCGGCTCAAGGAAGTGATGACCAAATCCTCAGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTGCCCTGTTCTGTGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCCCATGCCGACTCGGCCAATGACCTGCGACT
GCTGATCAAGCTTTCGGGTCATGAACAGCTGGGATCCGGCATGCTGGACAATGTGACGCTAGACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.689

95.122

0.558
  pilU Acinetobacter baylyi ADP1

56.091

95.664

0.537
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377