Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MRO13_RS11730 Genome accession   NZ_CP095311
Coordinates   2561418..2562455 (-) Length   345 a.a.
NCBI ID   WP_154169236.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 20CO01TI02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2556418..2567455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRO13_RS11700 (MRO13_11645) - 2556493..2557206 (+) 714 WP_161439132.1 endonuclease -
  MRO13_RS11705 (MRO13_11650) rsmE 2557507..2558238 (+) 732 WP_342651709.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MRO13_RS11710 (MRO13_11655) gshB 2558253..2559209 (+) 957 WP_170906572.1 glutathione synthase -
  MRO13_RS11715 (MRO13_11660) - 2559249..2559812 (+) 564 WP_040904706.1 YqgE/AlgH family protein -
  MRO13_RS11720 (MRO13_11665) ruvX 2559873..2560298 (+) 426 WP_004396054.1 Holliday junction resolvase RuvX -
  MRO13_RS11725 (MRO13_11670) pilU 2560301..2561407 (-) 1107 WP_187985462.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MRO13_RS11730 (MRO13_11675) pilT 2561418..2562455 (-) 1038 WP_154169236.1 type IV pilus twitching motility protein PilT Machinery gene
  MRO13_RS11735 (MRO13_11680) - 2562482..2563198 (+) 717 WP_186463503.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRO13_RS11740 (MRO13_11685) proC 2563281..2564099 (+) 819 WP_004396058.1 pyrroline-5-carboxylate reductase -
  MRO13_RS11745 (MRO13_11690) - 2564152..2564709 (+) 558 WP_170904736.1 YggT family protein -
  MRO13_RS11750 (MRO13_11695) yggU 2564709..2565002 (+) 294 WP_004396060.1 DUF167 family protein YggU -
  MRO13_RS11755 (MRO13_11700) - 2565095..2565700 (+) 606 WP_158137781.1 XTP/dITP diphosphatase -
  MRO13_RS11760 (MRO13_11705) hemW 2565694..2566863 (+) 1170 WP_170904737.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38232.67 Da        Isoelectric Point: 6.8231

>NTDB_id=676302 MRO13_RS11730 WP_154169236.1 2561418..2562455(-) (pilT) [Vibrio metschnikovii strain 20CO01TI02]
MDISELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFSHADVHRLIFAIMNDAQRSEYEERLEVDFSFELPNVGR
FRVNAFHQSRGSSAVFRTIPTVIPTLDQLNAPEIFRKISNYEKGLVLVTGPTGSGKSTTLAAMVDYINTHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLFKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQHA
KQLVAQGVVDLPEVQQKLGLDSSSF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=676302 MRO13_RS11730 WP_154169236.1 2561418..2562455(-) (pilT) [Vibrio metschnikovii strain 20CO01TI02]
ATGGATATCTCAGAGTTACTGGATTTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCAGGTGTTCCGCCCAT
GGTCCGGATTGATGGTGAAGTCCGTAAGCTGGGAGTGCCTGCTTTTAGTCATGCTGACGTGCATCGATTAATCTTTGCCA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGCGTCTCGAAGTCGATTTTTCCTTTGAGCTACCGAATGTGGGACGT
TTTCGGGTTAATGCCTTTCATCAGTCGCGCGGCAGCTCCGCCGTATTTCGAACGATTCCTACAGTGATCCCAACGTTGGA
TCAGTTGAATGCTCCGGAGATCTTTCGAAAAATATCCAATTATGAGAAAGGGTTAGTCCTTGTTACTGGCCCCACTGGAT
CCGGTAAATCGACAACGTTAGCGGCAATGGTTGATTATATCAATACTCATCATAATAAACACATTTTGACCATTGAAGAT
CCGATAGAATTTGTGCATAGCAATAATAAGTGTTTGATTAACCAGCGTGAAGTTCATCGTGATACACATAGTTTTAAAAA
TGCGTTACGTTCGGCGCTGCGAGAAGACCCTGATGTGATCTTAGTCGGTGAGCTGCGTGATCAAGAGACAATCAGTTTAG
CGTTAACCGCCGCTGAAACGGGACATTTAGTGTTTGGTACGCTACACACTAGTTCTGCCGCAAAAACCATTGATCGGATT
ATCGATGTATTCCCGGGCAGCGATAAAGACATGGTGCGTTCGATGTTATCGGAATCGCTGCGTGCGGTGATTGCGCAAAA
ACTGTTTAAGCGAGTCGGAGGGGGGCGCGTGGCGTGTCACGAAATTATGTTAGCGACTCCTGCGATTCGTAACTTGATCC
GAGAAGATAAAGTCGCACAGATGTATTCGATCATCCAAACGGGCGCTGCGCATGGGATGCAAACTATGGAGCAGCATGCT
AAGCAGTTGGTCGCTCAAGGGGTTGTGGATTTACCAGAGGTCCAACAAAAACTTGGTTTAGACAGCTCATCATTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.174

100

0.922
  pilT Vibrio cholerae strain A1552

92.174

100

0.922
  pilT Acinetobacter baumannii strain A118

70.145

100

0.701
  pilT Acinetobacter baumannii D1279779

70.145

100

0.701
  pilT Acinetobacter nosocomialis M2

69.855

100

0.699
  pilT Acinetobacter baylyi ADP1

72.289

96.232

0.696
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

67.953

97.681

0.664
  pilT Legionella pneumophila strain Lp02

67.781

95.362

0.646
  pilT Legionella pneumophila strain ERS1305867

67.781

95.362

0.646
  pilT Neisseria meningitidis 8013

63.006

100

0.632
  pilT Neisseria gonorrhoeae MS11

62.428

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.16

93.913

0.49
  pilU Vibrio cholerae strain A1552

43.692

94.203

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.606

95.652

0.388
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377