Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MRO13_RS11725 Genome accession   NZ_CP095311
Coordinates   2560301..2561407 (-) Length   368 a.a.
NCBI ID   WP_187985462.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 20CO01TI02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2555301..2566407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRO13_RS11695 (MRO13_11640) - 2555919..2556407 (+) 489 WP_158137784.1 SprT family zinc-dependent metalloprotease -
  MRO13_RS11700 (MRO13_11645) - 2556493..2557206 (+) 714 WP_161439132.1 endonuclease -
  MRO13_RS11705 (MRO13_11650) rsmE 2557507..2558238 (+) 732 WP_342651709.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MRO13_RS11710 (MRO13_11655) gshB 2558253..2559209 (+) 957 WP_170906572.1 glutathione synthase -
  MRO13_RS11715 (MRO13_11660) - 2559249..2559812 (+) 564 WP_040904706.1 YqgE/AlgH family protein -
  MRO13_RS11720 (MRO13_11665) ruvX 2559873..2560298 (+) 426 WP_004396054.1 Holliday junction resolvase RuvX -
  MRO13_RS11725 (MRO13_11670) pilU 2560301..2561407 (-) 1107 WP_187985462.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MRO13_RS11730 (MRO13_11675) pilT 2561418..2562455 (-) 1038 WP_154169236.1 type IV pilus twitching motility protein PilT Machinery gene
  MRO13_RS11735 (MRO13_11680) - 2562482..2563198 (+) 717 WP_186463503.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRO13_RS11740 (MRO13_11685) proC 2563281..2564099 (+) 819 WP_004396058.1 pyrroline-5-carboxylate reductase -
  MRO13_RS11745 (MRO13_11690) - 2564152..2564709 (+) 558 WP_170904736.1 YggT family protein -
  MRO13_RS11750 (MRO13_11695) yggU 2564709..2565002 (+) 294 WP_004396060.1 DUF167 family protein YggU -
  MRO13_RS11755 (MRO13_11700) - 2565095..2565700 (+) 606 WP_158137781.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41432.56 Da        Isoelectric Point: 5.8645

>NTDB_id=676301 MRO13_RS11725 WP_187985462.1 2560301..2561407(-) (pilU) [Vibrio metschnikovii strain 20CO01TI02]
MQLDQILQEMLTQGASDLYITVAAPILYRVNGDLQPQGEALNEAQVKELLLRMMDSDRQTEFNQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETQIPSFDDLQLPEVLKDLALTKRGLVLVVGATGSGKSTTMAAMTGYRNLKTRGHILTVEDPI
EFVHPHQGCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LMPKEKKEQFLFDLSMNLKGVIGQQLLRDKQGKGRHGVFEILLNSPRMADLIRRGDLHELKATMAKSKEVGMQTFDQALY
QLVIDDKITEQDALHSADSANDLRLMLKTQRGEGYSSGALDNIKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=676301 MRO13_RS11725 WP_187985462.1 2560301..2561407(-) (pilU) [Vibrio metschnikovii strain 20CO01TI02]
ATGCAATTGGATCAAATATTACAAGAGATGCTCACCCAAGGGGCGTCCGATCTCTACATCACTGTCGCGGCTCCAATTTT
GTATCGCGTTAATGGCGATCTCCAACCTCAGGGGGAAGCGTTAAATGAAGCTCAGGTCAAAGAGTTGTTATTGCGGATGA
TGGATAGCGACCGACAAACGGAGTTTAACCAAACGCGTGAGGCCAATTTTGCGATTGTGCGTGATTTTGGCCGTTTCCGA
GTCAGTGCCTTTTTTCAGCGTGAGCTACCGGGTGCCGTTATTCGCCGGATCGAAACGCAAATTCCTAGTTTTGATGACTT
GCAGTTGCCGGAGGTGCTTAAAGATCTCGCGCTAACCAAGCGGGGTTTAGTGTTAGTGGTCGGTGCGACAGGCTCGGGAA
AATCAACCACCATGGCGGCGATGACCGGTTATCGTAATTTGAAAACCCGCGGGCACATTCTTACTGTCGAGGATCCGATT
GAATTTGTTCATCCTCATCAAGGCTGTATTGTGACTCAGCGTGAAGTCGGCTTAGATACTGAGAGCTATGAAGTGGCATT
GAAAAATTCACTTCGCCAAGCGCCGGACATGATTTTGATTGGTGAAATACGCAGTCGTGAAACCATGGAATACGCGATGA
CCTTTGCCGAAACTGGGCATTTATGTATGGCAACCTTACACGCCAACAATGCCAACCAAGCCTTAGAGCGTATTTTACAT
TTGATGCCGAAAGAGAAAAAAGAACAGTTTCTGTTTGATTTATCGATGAACTTAAAAGGAGTGATTGGTCAGCAACTGCT
GCGTGATAAACAAGGTAAAGGGCGTCATGGCGTGTTTGAGATTTTGCTCAATAGTCCAAGAATGGCGGATTTGATCCGCC
GTGGCGATTTGCACGAATTAAAAGCAACCATGGCTAAATCGAAAGAAGTGGGAATGCAAACCTTCGATCAAGCGCTGTAT
CAATTGGTGATTGACGATAAAATCACTGAGCAAGATGCGCTGCACAGCGCCGATTCAGCCAATGATTTACGCCTTATGCT
CAAAACCCAGCGGGGAGAGGGTTACTCTTCTGGTGCGTTGGATAATATTAAGATTGATATGGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.152

100

0.832
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

52.991

95.38

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.667

97.826

0.408
  pilT Legionella pneumophila strain ERS1305867

40.413

92.12

0.372
  pilT Legionella pneumophila strain Lp02

40.413

92.12

0.372
  pilT Pseudomonas stutzeri DSM 10701

39.823

92.12

0.367
  pilT Pseudomonas aeruginosa PAK

39.823

92.12

0.367
  pilT Acinetobacter baumannii D1279779

39.528

92.12

0.364
  pilT Acinetobacter baumannii strain A118

39.528

92.12

0.364
  pilT Acinetobacter nosocomialis M2

39.528

92.12

0.364