Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MUU77_RS04715 Genome accession   NZ_CP095187
Coordinates   1030981..1032018 (+) Length   345 a.a.
NCBI ID   WP_093490243.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. F37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1025981..1037018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUU77_RS04685 (MUU77_04685) aac(6') 1026232..1026705 (+) 474 WP_245092121.1 aminoglycoside 6'-N-acetyltransferase -
  MUU77_RS04690 (MUU77_04690) - 1026709..1027503 (+) 795 WP_245092123.1 cobalamin-binding protein -
  MUU77_RS04695 (MUU77_04695) - 1027852..1028775 (+) 924 WP_245087217.1 transposase -
  MUU77_RS04700 (MUU77_04700) - 1028809..1029270 (-) 462 WP_245092125.1 DUF4426 domain-containing protein -
  MUU77_RS04705 (MUU77_04705) proC 1029331..1030173 (-) 843 WP_245092137.1 pyrroline-5-carboxylate reductase -
  MUU77_RS04710 (MUU77_04710) - 1030204..1030887 (-) 684 WP_245094236.1 YggS family pyridoxal phosphate-dependent enzyme -
  MUU77_RS04715 (MUU77_04715) pilT 1030981..1032018 (+) 1038 WP_093490243.1 type IV pilus twitching motility protein PilT Machinery gene
  MUU77_RS04720 (MUU77_04720) pilU 1032150..1033280 (+) 1131 WP_245092139.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MUU77_RS04725 (MUU77_04725) - 1033624..1036746 (-) 3123 WP_245092140.1 TonB-dependent receptor -
  MUU77_RS04730 (MUU77_04730) - 1036745..1036888 (+) 144 WP_245092142.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38560.31 Da        Isoelectric Point: 6.7271

>NTDB_id=676035 MUU77_RS04715 WP_093490243.1 1030981..1032018(+) (pilT) [Pseudoxanthomonas sp. F37]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPLFRELIQQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
IDVFPAGEKPMVRSMLSESLRAVISQALLKKIGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQSGMMTLDQHL
QDLVKRALITRQQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=676035 MUU77_RS04715 WP_093490243.1 1030981..1032018(+) (pilT) [Pseudoxanthomonas sp. F37]
ATGGATATCGCCGAACTTCTGGCGTTCTCGGTAAAGAACAAGGCCTCCGACCTCCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGGGTGGACGGCGACGTGCGCCGCATCAACATCCCGGCGCTGGACCACAAGCAGGTGCATGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTTCGAGGAGTTCCTGGAAGTCGACTTCTCGTTCGAGATCCCGTCGCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGTGGCGCCGGCGCCGTCTTCCGCACGATTCCTTCGGAAGTGCTGACCCTGGA
AGACCTGGGCTGCCCGCCGCTGTTCCGCGAGCTGATCCAGCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCATATCAACAAGAACGAGTACGGGCACATCCTCAGCGTCGAGGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAAGACCCGGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACGGGCCACCTGGTGTTCGCCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCGTG
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCCATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGATCGGCGGTGGCCGTACCGCGGCGTGGGAAATCATGGTGGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGAGCGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTGAAGCGCGCGCTGATCACCCGCCAGCAGGCCAAGGAATACGCGAAGGACAAGCGTCTGTTCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Acinetobacter baylyi ADP1

76.163

99.71

0.759
  pilT Legionella pneumophila strain Lp02

73.547

99.71

0.733
  pilT Legionella pneumophila strain ERS1305867

73.547

99.71

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.06

97.101

0.661
  pilT Vibrio cholerae strain A1552

68.06

97.101

0.661
  pilT Neisseria meningitidis 8013

64.223

98.841

0.635
  pilT Neisseria gonorrhoeae MS11

63.93

98.841

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.003

98.261

0.403
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397