Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MUU77_RS04720 Genome accession   NZ_CP095187
Coordinates   1032150..1033280 (+) Length   376 a.a.
NCBI ID   WP_245092139.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. F37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1027150..1038280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUU77_RS04695 (MUU77_04695) - 1027852..1028775 (+) 924 WP_245087217.1 transposase -
  MUU77_RS04700 (MUU77_04700) - 1028809..1029270 (-) 462 WP_245092125.1 DUF4426 domain-containing protein -
  MUU77_RS04705 (MUU77_04705) proC 1029331..1030173 (-) 843 WP_245092137.1 pyrroline-5-carboxylate reductase -
  MUU77_RS04710 (MUU77_04710) - 1030204..1030887 (-) 684 WP_245094236.1 YggS family pyridoxal phosphate-dependent enzyme -
  MUU77_RS04715 (MUU77_04715) pilT 1030981..1032018 (+) 1038 WP_093490243.1 type IV pilus twitching motility protein PilT Machinery gene
  MUU77_RS04720 (MUU77_04720) pilU 1032150..1033280 (+) 1131 WP_245092139.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MUU77_RS04725 (MUU77_04725) - 1033624..1036746 (-) 3123 WP_245092140.1 TonB-dependent receptor -
  MUU77_RS04730 (MUU77_04730) - 1036745..1036888 (+) 144 WP_245092142.1 hypothetical protein -
  MUU77_RS04735 (MUU77_04735) - 1037517..1037960 (+) 444 WP_245092144.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41891.25 Da        Isoelectric Point: 6.8287

>NTDB_id=676036 MUU77_RS04720 WP_245092139.1 1032150..1033280(+) (pilU) [Pseudoxanthomonas sp. F37]
MSTIDFTSFLKLMAHQKASDLFITSGMPPSIKVHGKISPITQTPLTAQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTIEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRTQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=676036 MUU77_RS04720 WP_245092139.1 1032150..1033280(+) (pilU) [Pseudoxanthomonas sp. F37]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACGTCCGGCAT
GCCGCCGTCCATCAAGGTGCACGGCAAGATTTCGCCGATCACCCAGACTCCGCTGACGGCGCAGCAGTCGCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAATTCGAAAAGACCCACGAATGCAACTTCGCCATCGGCGTGGCCGGC
GTGGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGCATCGAGACGCGCATCCC
GACCATCGAAGAGCTGAACCTGCCGCCGGTCATCAAGACGCTGGCCATGACCAAGCGCGGCATCATCATCTTCGTGGGCG
CCACCGGCACCGGCAAGTCCACGTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACC
ATCGAAGACCCCATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGTGAAGTCGGCATCGACACCGACAG
CTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCACCCAGCTGCTGATGGACCTGTCGCTCAACCTGAAGGGCGTGGT
GGCGCAGCAGCTGATCCCCACGCCGGACGGCAAGGGGCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCTATGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAACTGGCCCAGGGTGGCGATGCCCGCACGCTGGCGCAGGGCCTGGATGGCGTGGAGATCGCGG
AAGTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

97.606

0.662
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

54.213

94.681

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

93.085

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362