Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L9B60_RS13430 Genome accession   NZ_CP094975
Coordinates   2959380..2960414 (+) Length   344 a.a.
NCBI ID   WP_249679289.1    Uniprot ID   -
Organism   Pseudomonas abieticivorans strain PIA16     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2954380..2965414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L9B60_RS13400 - 2954862..2955299 (-) 438 WP_249679283.1 DUF4426 domain-containing protein -
  L9B60_RS13405 metW 2955321..2955941 (-) 621 WP_249679284.1 methionine biosynthesis protein MetW -
  L9B60_RS13410 - 2955949..2957088 (-) 1140 WP_249679285.1 homoserine O-acetyltransferase -
  L9B60_RS13415 - 2957183..2957773 (-) 591 WP_249679286.1 YggT family protein -
  L9B60_RS13420 proC 2957784..2958602 (-) 819 WP_249679287.1 pyrroline-5-carboxylate reductase -
  L9B60_RS13425 - 2958628..2959314 (-) 687 WP_249679288.1 YggS family pyridoxal phosphate-dependent enzyme -
  L9B60_RS13430 pilT 2959380..2960414 (+) 1035 WP_249679289.1 type IV pilus twitching motility protein PilT Machinery gene
  L9B60_RS13435 pilU 2960476..2961597 (+) 1122 WP_249679290.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L9B60_RS13440 - 2961598..2962263 (-) 666 WP_249679291.1 C40 family peptidase -
  L9B60_RS13445 - 2962512..2962931 (+) 420 WP_249679292.1 TM2 domain-containing protein -
  L9B60_RS13450 - 2963083..2964357 (-) 1275 WP_249679293.1 dihydroorotase -
  L9B60_RS13455 - 2964354..2965358 (-) 1005 WP_249679294.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38050.81 Da        Isoelectric Point: 6.6401

>NTDB_id=674651 L9B60_RS13430 WP_249679289.1 2959380..2960414(+) (pilT) [Pseudomonas abieticivorans strain PIA16]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHELIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMIDYLNNNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLHAVISQTLMKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDMCL
KDLVNKGVIGRDAAREKAKTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=674651 L9B60_RS13430 WP_249679289.1 2959380..2960414(+) (pilT) [Pseudomonas abieticivorans strain PIA16]
ATGGACATTACCGAGCTGTTGGCTTTCAGTGCGAAGCAGGGGGCTTCGGACTTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATTCGGGTGGACGGCGATGTGCGGCGGATCAACCTGCCGCCACTGGACCACAAACAGGTCCACGAGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAGTTCCTGGAAACCGACTTCTCCTTCGAAGTGCCGGGTGTCGCGCGG
TTTCGGGTCAACGCGTTCAACCAGAACCGTGGCGCTGGTGCAGTATTCCGTACCATCCCCTCCAAGGTGCTGACCATGGA
AGACTTGGGGATGGGAGAAGTGTTTCGCAAGATTACCGACGTGCCTCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CCGGCAAGTCGACCACCCTGGCGGCGATGATCGATTACCTGAACAACAACAAACACCATCACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTGCACGAGTCCAAAAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCTGGGTTTTTCCGA
GGCGCTGCGCTCGGCCTTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTCACCGCCGCCGAAACCGGCCACTTGGTATTTGGCACGCTGCACACCACGTCGGCCGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCGCCGAAGAGAAGTCCATGGTCCGCTCGATGCTTTCCGAGTCGCTGCATGCCGTGATCTCGCAAAC
CCTGATGAAAAAAATTGGTGGTGGCCGCGTGGCGGCCCACGAAATCATGATCGGCACCCCGGCCATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCGGTGCATTGGGCATGCAGACCCTGGACATGTGCCTC
AAGGACTTGGTGAACAAGGGCGTGATCGGCCGTGACGCGGCTCGCGAAAAGGCCAAGACGCCCGACAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

93.605

100

0.936
  pilT Pseudomonas aeruginosa PAK

93.314

100

0.933
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baumannii D1279779

83.14

100

0.831
  pilT Acinetobacter baumannii strain A118

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

81.105

100

0.811
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Vibrio cholerae strain A1552

39.71

100

0.398