Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L9B60_RS13435 Genome accession   NZ_CP094975
Coordinates   2960476..2961597 (+) Length   373 a.a.
NCBI ID   WP_249679290.1    Uniprot ID   -
Organism   Pseudomonas abieticivorans strain PIA16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2955476..2966597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L9B60_RS13410 - 2955949..2957088 (-) 1140 WP_249679285.1 homoserine O-acetyltransferase -
  L9B60_RS13415 - 2957183..2957773 (-) 591 WP_249679286.1 YggT family protein -
  L9B60_RS13420 proC 2957784..2958602 (-) 819 WP_249679287.1 pyrroline-5-carboxylate reductase -
  L9B60_RS13425 - 2958628..2959314 (-) 687 WP_249679288.1 YggS family pyridoxal phosphate-dependent enzyme -
  L9B60_RS13430 pilT 2959380..2960414 (+) 1035 WP_249679289.1 type IV pilus twitching motility protein PilT Machinery gene
  L9B60_RS13435 pilU 2960476..2961597 (+) 1122 WP_249679290.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L9B60_RS13440 - 2961598..2962263 (-) 666 WP_249679291.1 C40 family peptidase -
  L9B60_RS13445 - 2962512..2962931 (+) 420 WP_249679292.1 TM2 domain-containing protein -
  L9B60_RS13450 - 2963083..2964357 (-) 1275 WP_249679293.1 dihydroorotase -
  L9B60_RS13455 - 2964354..2965358 (-) 1005 WP_249679294.1 aspartate carbamoyltransferase catalytic subunit -
  L9B60_RS13460 pyrR 2965371..2965889 (-) 519 WP_249679295.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  L9B60_RS13465 ruvX 2965942..2966382 (-) 441 WP_249679296.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41360.61 Da        Isoelectric Point: 6.9358

>NTDB_id=674652 L9B60_RS13435 WP_249679290.1 2960476..2961597(+) (pilU) [Pseudomonas abieticivorans strain PIA16]
MEFHKLLRLMVDKGGSDLFITPGLPPSLKVHGKILPVTKQSLSAEQARDAVLGLMTEVQRLEFAQQHESNFAIHARDIGR
FRVSAFYQRNQPAMVLRRIESHIPSFEELKLPEGLKALAMIKRGLVLFVGATGTGKSSSLAAMIDYRSKNSTGHIITIED
PIEYLHEHQGCIVTQREVGIDTESFEVALRNTLRQAPDVIMIGEVRSRETMGQALTFAETGHLCLATLHASNADQALERV
IHFFPVDRHPQVWMELSLNLRAIVAQQLIPTSDGSGRRAVVEVLINTPLVADIIRKGQIPGVKPVMKRSSGQGMRTLDQA
LYELYQEQAISYEDALSYADSANDLRLMIKLGKGAVQGEELNMTPEDPAPPSR

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=674652 L9B60_RS13435 WP_249679290.1 2960476..2961597(+) (pilU) [Pseudomonas abieticivorans strain PIA16]
ATGGAGTTTCACAAACTGCTACGTCTGATGGTCGACAAAGGCGGCTCCGACCTGTTTATCACCCCAGGTCTGCCGCCCTC
GCTCAAAGTGCATGGCAAGATCCTGCCGGTGACCAAGCAGTCGCTCAGTGCCGAACAGGCCCGCGATGCGGTGTTGGGCC
TGATGACCGAGGTGCAGCGGCTGGAGTTCGCCCAGCAGCACGAGAGCAACTTCGCCATCCATGCCCGTGACATCGGCCGG
TTTCGGGTCAGCGCGTTCTACCAGCGCAACCAGCCGGCCATGGTGCTACGACGCATCGAGAGCCATATCCCCAGCTTTGA
AGAGCTTAAATTGCCCGAGGGGCTCAAGGCCCTGGCGATGATCAAGCGCGGGCTGGTGCTGTTCGTGGGGGCCACGGGCA
CTGGCAAGTCCTCGTCGCTGGCGGCGATGATCGATTACCGCAGCAAGAACAGCACCGGGCACATCATCACCATCGAAGAC
CCCATCGAGTACCTTCACGAGCATCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGCATCGACACCGAGAGTTTCGAGGT
GGCGTTACGCAACACCCTGCGCCAGGCACCGGATGTGATCATGATCGGCGAGGTGCGCAGCCGCGAGACCATGGGCCAGG
CGCTGACCTTCGCCGAGACTGGCCACCTGTGCCTGGCCACCTTGCACGCCTCCAACGCCGACCAGGCATTGGAGCGGGTG
ATCCATTTCTTCCCGGTGGACCGCCACCCGCAGGTGTGGATGGAACTGTCGCTGAACCTGCGTGCCATCGTCGCCCAGCA
GTTGATTCCCACCAGTGATGGCAGCGGCCGGCGTGCGGTGGTGGAAGTGCTGATCAATACCCCGCTGGTGGCCGACATTA
TCCGCAAGGGCCAGATCCCCGGGGTAAAGCCGGTGATGAAGCGCTCCAGCGGGCAGGGCATGCGTACCCTGGACCAGGCG
TTGTATGAGTTGTATCAGGAGCAAGCCATAAGCTATGAAGACGCCTTGTCCTATGCCGATTCGGCAAATGACTTGCGGTT
GATGATCAAGCTGGGCAAGGGGGCGGTGCAGGGCGAGGAGCTGAACATGACGCCGGAGGACCCGGCGCCACCTTCGCGCT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.816

100

0.713
  pilU Acinetobacter baylyi ADP1

62.079

95.442

0.592
  pilU Vibrio cholerae strain A1552

54.743

98.928

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.978

99.732

0.389
  pilT Neisseria meningitidis 8013

39.42

92.493

0.365
  pilT Legionella pneumophila strain Lp02

40.299

89.812

0.362
  pilT Legionella pneumophila strain ERS1305867

40.299

89.812

0.362
  pilT Neisseria gonorrhoeae MS11

39.13

92.493

0.362