Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MTZ49_RS14745 Genome accession   NZ_CP094972
Coordinates   3113984..3115000 (-) Length   338 a.a.
NCBI ID   WP_264746209.1    Uniprot ID   -
Organism   Entomomonas sp. E2T0     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3108984..3120000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTZ49_RS14720 (MTZ49_14715) - 3109612..3110478 (-) 867 WP_264746204.1 alpha/beta fold hydrolase -
  MTZ49_RS14725 (MTZ49_14720) - 3110634..3111230 (+) 597 WP_264746205.1 IMPACT family protein -
  MTZ49_RS14730 (MTZ49_14725) - 3111307..3111780 (+) 474 WP_264746206.1 hypothetical protein -
  MTZ49_RS14735 (MTZ49_14730) - 3111832..3112725 (+) 894 WP_264746207.1 toxin-antitoxin system YwqK family antitoxin -
  MTZ49_RS14740 (MTZ49_14735) pilU 3112779..3113918 (-) 1140 WP_264746208.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MTZ49_RS14745 (MTZ49_14740) pilT 3113984..3115000 (-) 1017 WP_264746209.1 type IV pilus twitching motility protein PilT Machinery gene
  MTZ49_RS14750 (MTZ49_14745) - 3115110..3115805 (+) 696 WP_264746210.1 YggS family pyridoxal phosphate-dependent enzyme -
  MTZ49_RS14755 (MTZ49_14750) - 3115891..3116442 (+) 552 WP_264746211.1 YggT family protein -
  MTZ49_RS14760 (MTZ49_14755) metX 3116485..3117627 (+) 1143 WP_264746212.1 homoserine O-succinyltransferase MetX -
  MTZ49_RS14765 (MTZ49_14760) metW 3117638..3118234 (+) 597 WP_264746213.1 methionine biosynthesis protein MetW -
  MTZ49_RS14770 (MTZ49_14765) rdgB 3118254..3118847 (+) 594 WP_264746214.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  MTZ49_RS14775 (MTZ49_14770) hemW 3118847..3119998 (+) 1152 WP_264746215.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37529.26 Da        Isoelectric Point: 6.6664

>NTDB_id=674638 MTZ49_RS14745 WP_264746209.1 3113984..3115000(-) (pilT) [Entomomonas sp. E2T0]
MEITELLAFSAEHKASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHSLVYDIMNDRQRKEYEENLETDFSVEVPGLAR
FRVNAFNQNRGAAAVFRTIPSKVLTMTELGMGDVFEKICNNERGLVLVTGPTGSGKSTTLAAMVDYVNATRYEHILTIED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKSMIRSMLSESLQAVVSQKLIKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMNSSIQTGAQVGMKTMDSSL
RLLVEKGLIERIYLKKAD

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=674638 MTZ49_RS14745 WP_264746209.1 3113984..3115000(-) (pilT) [Entomomonas sp. E2T0]
ATGGAAATCACTGAATTACTAGCGTTTAGCGCAGAGCACAAAGCATCGGACTTACATTTATCTGCAGGATTGCCACCAAT
GATTCGTGTGGATGGGGATGTACGCCGTATAAACCTTCCACCTTTAGATCATAAGCAAGTTCATAGCCTTGTTTACGATA
TTATGAATGATAGGCAACGTAAGGAGTATGAAGAAAACTTGGAGACGGACTTTTCTGTTGAAGTTCCAGGTTTAGCCCGT
TTTCGGGTAAATGCTTTTAATCAAAATCGTGGGGCTGCAGCAGTATTTCGTACCATTCCTTCTAAAGTGTTAACAATGAC
AGAATTAGGGATGGGTGATGTATTTGAGAAGATTTGTAATAATGAGCGCGGTTTAGTGCTGGTTACAGGTCCTACAGGTT
CTGGTAAATCAACCACACTAGCAGCGATGGTGGATTATGTGAATGCAACACGCTATGAGCATATTTTAACCATTGAGGAC
CCAATTGAGTTTGTTCATGAGTCTAAAAAATGCCTTGTAAACCAGCGTGAAGTTCATAGAGATACCTTAGGGTTTAATGA
GGCATTACGTAGTGCATTGCGTGAAGACCCGGATATTATCCTCGTAGGTGAAATGCGTGACTTGGAAACTATTCGTTTGG
CATTAACTGCGGCAGAAACAGGTCACTTGGTATTTGGTACATTACATACCAGTTCAGCAGCAAAAACTATTGACCGTATT
ATTGATGTATTCCCAGCAGCTGAAAAGTCAATGATTCGCTCAATGCTTTCTGAGTCATTACAAGCAGTAGTTTCACAGAA
GTTAATTAAAAAGATTGGCGGTGGTCGAGTAGCTGCTCATGAAATTATGATTGGTACACCGGCTATTAGAAACTTAATTC
GTGAAGATAAAGTAGCACAGATGAATTCTTCTATTCAAACTGGAGCGCAGGTAGGTATGAAAACGATGGATTCTAGTTTA
AGGCTTCTAGTAGAAAAAGGCCTTATTGAAAGGATTTATTTAAAGAAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.592

97.929

0.828
  pilT Pseudomonas aeruginosa PAK

84.592

97.929

0.828
  pilT Acinetobacter baylyi ADP1

81.155

97.337

0.79
  pilT Acinetobacter nosocomialis M2

80.851

97.337

0.787
  pilT Acinetobacter baumannii D1279779

80.851

97.337

0.787
  pilT Acinetobacter baumannii strain A118

80.851

97.337

0.787
  pilT Legionella pneumophila strain Lp02

75.529

97.929

0.74
  pilT Legionella pneumophila strain ERS1305867

75.529

97.929

0.74
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.697

97.633

0.68
  pilT Vibrio cholerae strain A1552

69.697

97.633

0.68
  pilT Neisseria meningitidis 8013

68.788

97.633

0.672
  pilT Neisseria gonorrhoeae MS11

68.485

97.633

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.03

97.633

0.518
  pilU Vibrio cholerae strain A1552

41.176

100

0.414
  pilU Pseudomonas stutzeri DSM 10701

41.818

97.633

0.408
  pilU Acinetobacter baylyi ADP1

39.13

95.266

0.373