Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MTZ49_RS14740 Genome accession   NZ_CP094972
Coordinates   3112779..3113918 (-) Length   379 a.a.
NCBI ID   WP_264746208.1    Uniprot ID   -
Organism   Entomomonas sp. E2T0     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3107779..3118918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTZ49_RS14710 (MTZ49_14705) - 3108050..3108361 (-) 312 WP_264746202.1 SCP2 sterol-binding domain-containing protein -
  MTZ49_RS14715 (MTZ49_14710) sohB 3108541..3109569 (+) 1029 WP_264746203.1 protease SohB -
  MTZ49_RS14720 (MTZ49_14715) - 3109612..3110478 (-) 867 WP_264746204.1 alpha/beta fold hydrolase -
  MTZ49_RS14725 (MTZ49_14720) - 3110634..3111230 (+) 597 WP_264746205.1 IMPACT family protein -
  MTZ49_RS14730 (MTZ49_14725) - 3111307..3111780 (+) 474 WP_264746206.1 hypothetical protein -
  MTZ49_RS14735 (MTZ49_14730) - 3111832..3112725 (+) 894 WP_264746207.1 toxin-antitoxin system YwqK family antitoxin -
  MTZ49_RS14740 (MTZ49_14735) pilU 3112779..3113918 (-) 1140 WP_264746208.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MTZ49_RS14745 (MTZ49_14740) pilT 3113984..3115000 (-) 1017 WP_264746209.1 type IV pilus twitching motility protein PilT Machinery gene
  MTZ49_RS14750 (MTZ49_14745) - 3115110..3115805 (+) 696 WP_264746210.1 YggS family pyridoxal phosphate-dependent enzyme -
  MTZ49_RS14755 (MTZ49_14750) - 3115891..3116442 (+) 552 WP_264746211.1 YggT family protein -
  MTZ49_RS14760 (MTZ49_14755) metX 3116485..3117627 (+) 1143 WP_264746212.1 homoserine O-succinyltransferase MetX -
  MTZ49_RS14765 (MTZ49_14760) metW 3117638..3118234 (+) 597 WP_264746213.1 methionine biosynthesis protein MetW -
  MTZ49_RS14770 (MTZ49_14765) rdgB 3118254..3118847 (+) 594 WP_264746214.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42139.71 Da        Isoelectric Point: 6.0030

>NTDB_id=674637 MTZ49_RS14740 WP_264746208.1 3112779..3113918(-) (pilU) [Entomomonas sp. E2T0]
MEFEKLLQLMVDKKASDLFLTVGVPPCIKVDGKLAPLPVDKLNGDRMKELARLLMTERQQKEFASTQECNFAVSYPGIGR
FRVSAYIQRNQVGMVLRRIVTEIPSLEQLALPKIIKDLAMVKRGLVLFVGATGAGKSTSLAAMVDYRNTHSTGHIISIED
PIEYLFNHKNCIVTQREVGLDTESFEVALKNTLRQAPDVIMIGEVRTRQTMEYALAFAETGHLCLATLHANNANQALDRI
ISFFPTERHSQVWLDLSLNLRAIVAQQLIPNKSGVGRQAAVEVLLGTPLVSDMIRTGQVTELKGIMSRSVELGMQTFDQA
LFDLFERGFISYESALACADSVNDLRLMIKLNSEKSVKASSSELSGVKLKDYDDEEDDI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=674637 MTZ49_RS14740 WP_264746208.1 3112779..3113918(-) (pilU) [Entomomonas sp. E2T0]
ATGGAGTTTGAGAAATTATTACAGTTAATGGTGGATAAAAAAGCCTCTGACTTATTTTTGACAGTGGGTGTTCCGCCCTG
TATAAAAGTTGATGGTAAGTTAGCTCCATTACCTGTGGATAAACTGAACGGCGATAGAATGAAAGAGTTGGCTAGGTTAT
TAATGACTGAACGCCAACAAAAAGAGTTTGCTTCTACACAGGAGTGTAACTTCGCTGTTAGTTATCCTGGAATTGGGCGT
TTTAGGGTGAGTGCTTATATTCAGCGTAATCAAGTGGGCATGGTATTACGCCGGATTGTTACAGAGATTCCTAGTCTGGA
ACAGTTAGCATTACCTAAAATTATTAAAGACTTAGCCATGGTAAAACGTGGCTTAGTATTGTTCGTAGGGGCAACAGGGG
CAGGTAAATCTACCTCACTAGCAGCTATGGTGGATTATCGCAATACCCATTCGACAGGGCATATTATTTCAATTGAAGAC
CCCATTGAATATTTGTTTAATCATAAGAACTGTATTGTAACTCAACGGGAAGTTGGGCTTGATACTGAGTCATTTGAAGT
CGCTCTTAAAAATACCTTACGCCAGGCTCCTGATGTGATCATGATTGGTGAGGTACGTACTCGCCAAACCATGGAGTATG
CGTTGGCGTTTGCTGAAACAGGCCACTTATGTTTAGCAACCTTGCATGCTAATAATGCTAACCAAGCATTAGATCGTATT
ATCAGCTTTTTCCCAACTGAACGTCATTCACAAGTATGGTTAGATTTATCATTAAATTTACGAGCTATTGTAGCGCAGCA
ATTAATTCCTAATAAAAGTGGTGTGGGACGGCAAGCTGCCGTTGAAGTATTATTAGGTACGCCACTTGTATCTGATATGA
TTCGTACAGGTCAGGTAACCGAGTTAAAAGGTATTATGTCACGTTCTGTTGAGTTGGGGATGCAAACCTTTGACCAAGCT
TTATTTGATCTATTTGAAAGAGGCTTCATCTCTTATGAATCTGCTTTAGCTTGTGCAGACTCTGTGAACGATTTACGTTT
GATGATTAAATTAAACTCAGAGAAAAGTGTTAAAGCTTCTTCAAGTGAATTATCAGGAGTCAAACTAAAAGACTATGATG
ATGAAGAGGATGATATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.867

98.945

0.652
  pilU Acinetobacter baylyi ADP1

60.563

93.668

0.567
  pilU Vibrio cholerae strain A1552

55.827

97.361

0.544
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.183

89.446

0.377
  pilT Legionella pneumophila strain ERS1305867

41.003

89.446

0.367
  pilT Legionella pneumophila strain Lp02

41.003

89.446

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

87.071

0.367
  pilT Vibrio cholerae strain A1552

42.121

87.071

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

88.391

0.361
  pilT Acinetobacter baylyi ADP1

40.176

89.974

0.361