Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SVA_RS17370 Genome accession   NZ_AP014936
Coordinates   3584311..3585405 (-) Length   364 a.a.
NCBI ID   WP_096462408.1    Uniprot ID   A0A1C7AFH4
Organism   Sulfurifustis variabilis strain skN76     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3579311..3590405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SVA_RS17345 (SVA_3533) - 3579370..3580419 (-) 1050 WP_096462403.1 hypothetical protein -
  SVA_RS17350 (SVA_3534) - 3580556..3581080 (-) 525 WP_096462404.1 hypothetical protein -
  SVA_RS17355 (SVA_3535) - 3581186..3582049 (-) 864 WP_096462405.1 IS3 family transposase -
  SVA_RS17360 (SVA_3536) - 3582046..3582375 (-) 330 WP_096462406.1 transposase -
  SVA_RS17365 (SVA_3538) pilU 3583199..3584314 (-) 1116 WP_096462407.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SVA_RS17370 (SVA_3539) pilT 3584311..3585405 (-) 1095 WP_096462408.1 type IV pilus twitching motility protein PilT Machinery gene
  SVA_RS17375 (SVA_3540) proC 3585539..3586456 (+) 918 WP_096462409.1 pyrroline-5-carboxylate reductase -
  SVA_RS17380 (SVA_3541) - 3586453..3587034 (+) 582 WP_096462410.1 YggT family protein -
  SVA_RS17385 (SVA_3542) - 3587145..3589103 (+) 1959 WP_169924163.1 dynamin family protein -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 39858.66 Da        Isoelectric Point: 6.3457

>NTDB_id=67437 SVA_RS17370 WP_096462408.1 3584311..3585405(-) (pilT) [Sulfurifustis variabilis strain skN76]
MDIAELLAFAFKQNASDLHLSSGLPPLLRVDGEIKRINVDPLDDRTVHNMVYDIMSDKQQKDFEEFLECDFSFELPGIAR
FRVNAFNQQRGPGAVFRTIPSKVLTLEQLNAPEIFKKICDQPRGLVLVTGPTGSGKSTTLAAMINHINETEHEHILTIED
PIEFVHTSKNCLINQREVGRDTLGFNNALRASLREDPDSILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRAMLSESLRAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREGKIAQMYSSIQTGQQFGMQTLDQCL
QELVRTRQVHVDDARTMAVNKESFGASAMAGAKAGGAAAGAKPA

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=67437 SVA_RS17370 WP_096462408.1 3584311..3585405(-) (pilT) [Sulfurifustis variabilis strain skN76]
ATGGATATCGCCGAACTGCTGGCGTTTGCCTTCAAACAGAACGCCTCGGACCTGCATCTGTCGTCCGGCCTGCCGCCGCT
GCTGCGCGTGGACGGGGAGATCAAGCGCATCAACGTCGATCCCCTGGACGACCGGACCGTCCACAACATGGTGTACGACA
TCATGTCGGACAAGCAGCAGAAGGACTTCGAGGAATTCCTCGAATGCGACTTCTCCTTCGAGCTGCCGGGGATCGCGCGC
TTCCGCGTCAACGCCTTCAACCAGCAGCGCGGACCCGGGGCCGTGTTCCGGACGATTCCTTCGAAGGTGCTGACCCTCGA
GCAGCTCAATGCCCCCGAGATCTTCAAGAAGATCTGCGACCAGCCGCGCGGGCTCGTGCTCGTGACGGGGCCGACGGGCT
CGGGCAAGTCGACCACGCTCGCGGCGATGATCAACCACATCAACGAGACCGAGCACGAGCACATCCTCACGATCGAGGAC
CCGATCGAGTTCGTGCACACGAGCAAGAACTGCCTCATCAACCAGCGCGAGGTCGGGCGGGACACGCTCGGCTTCAACAA
CGCGCTGCGCGCCTCGTTGCGCGAGGACCCGGACAGCATCCTGGTCGGCGAAATGCGCGATCTCGAGACCATCCGGCTCG
CGCTCACCGCGGCCGAGACGGGCCACCTCGTCTTCGGCACCCTGCACACGTCGTCGGCGGCGAAGACCATCGACCGTATC
ATCGACGTGTTCCCGGCGGCCGAGAAGGACATGGTGCGCGCGATGCTCTCGGAGAGCCTGCGCGCCGTCATCTCCCAGAC
GCTGCTCAAGAAGGTCGGCGGCGGGCGCGTTGCGGCGCACGAGATCATGATCGGCACGCCGGCGATCCGGAACCTGATCC
GCGAGGGCAAGATCGCGCAGATGTACTCGTCGATCCAGACCGGCCAGCAGTTCGGCATGCAGACGCTCGACCAGTGCCTG
CAGGAGCTGGTGCGCACGCGCCAGGTGCACGTCGACGACGCGCGCACGATGGCGGTCAACAAGGAGTCCTTCGGCGCCAG
CGCGATGGCGGGCGCCAAGGCCGGCGGCGCCGCCGCGGGGGCGAAGCCGGCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C7AFH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.872

94.505

0.717
  pilT Acinetobacter nosocomialis M2

75.291

94.505

0.712
  pilT Acinetobacter baumannii D1279779

75.291

94.505

0.712
  pilT Acinetobacter baumannii strain A118

75.291

94.505

0.712
  pilT Pseudomonas aeruginosa PAK

74.709

94.505

0.706
  pilT Acinetobacter baylyi ADP1

74.419

94.505

0.703
  pilT Legionella pneumophila strain Lp02

68.605

94.505

0.648
  pilT Legionella pneumophila strain ERS1305867

68.605

94.505

0.648
  pilT Neisseria meningitidis 8013

67.826

94.78

0.643
  pilT Neisseria gonorrhoeae MS11

67.536

94.78

0.64
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.062

89.011

0.624
  pilT Vibrio cholerae strain A1552

70.062

89.011

0.624
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.959

100

0.511
  pilU Vibrio cholerae strain A1552

42.09

92.033

0.387
  pilU Acinetobacter baylyi ADP1

41.964

92.308

0.387
  pilU Pseudomonas stutzeri DSM 10701

41.493

92.033

0.382

Multiple sequence alignment