Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SVA_RS17365 Genome accession   NZ_AP014936
Coordinates   3583199..3584314 (-) Length   371 a.a.
NCBI ID   WP_096462407.1    Uniprot ID   A0A1B4VBU0
Organism   Sulfurifustis variabilis strain skN76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3578199..3589314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SVA_RS19880 (SVA_3532) - 3578983..3579159 (-) 177 WP_169923907.1 hypothetical protein -
  SVA_RS17345 (SVA_3533) - 3579370..3580419 (-) 1050 WP_096462403.1 hypothetical protein -
  SVA_RS17350 (SVA_3534) - 3580556..3581080 (-) 525 WP_096462404.1 hypothetical protein -
  SVA_RS17355 (SVA_3535) - 3581186..3582049 (-) 864 WP_096462405.1 IS3 family transposase -
  SVA_RS17360 (SVA_3536) - 3582046..3582375 (-) 330 WP_096462406.1 transposase -
  SVA_RS17365 (SVA_3538) pilU 3583199..3584314 (-) 1116 WP_096462407.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SVA_RS17370 (SVA_3539) pilT 3584311..3585405 (-) 1095 WP_096462408.1 type IV pilus twitching motility protein PilT Machinery gene
  SVA_RS17375 (SVA_3540) proC 3585539..3586456 (+) 918 WP_096462409.1 pyrroline-5-carboxylate reductase -
  SVA_RS17380 (SVA_3541) - 3586453..3587034 (+) 582 WP_096462410.1 YggT family protein -
  SVA_RS17385 (SVA_3542) - 3587145..3589103 (+) 1959 WP_169924163.1 dynamin family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41617.92 Da        Isoelectric Point: 6.8438

>NTDB_id=67436 SVA_RS17365 WP_096462407.1 3583199..3584314(-) (pilU) [Sulfurifustis variabilis strain skN76]
MNFADLLKLMKHKRGSDLFITAGVPPSMKVDGKLMPVTKQALTPEQSRAFAYGIMNEEQRRQFEEHSECNFAIAPQEIGR
FRVNVFMQQQRVGMVLRTINTDIPRFDDLNLPKVLADVALTKRGLVLFVGGTGSGKSTSLAAMIGYRNEYSYGHILTVED
PIEYVHQHKNCIITQREVGVDTESYEVALKNAMRQAPDVILIGEIRSREAMDLGVQFAETGHLCMSTLHANNANQALDRI
INFFPEEKRNQLLMDLALNLKAIISQRLIPTKSGKGRVPAVEVLINTPLVSDMIMEGNIPGLKDLMAKSPEAGMQTFDQS
LFTLFEADLISYDDALRNADSVNDVRLRIKLESKDSKNRELGDTLRNVTYK

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=67436 SVA_RS17365 WP_096462407.1 3583199..3584314(-) (pilU) [Sulfurifustis variabilis strain skN76]
ATGAACTTCGCCGATCTCCTCAAGCTCATGAAGCACAAGAGGGGCTCGGACCTCTTCATCACGGCCGGCGTGCCGCCGTC
GATGAAGGTCGACGGCAAGCTCATGCCCGTGACCAAGCAGGCGCTCACGCCGGAGCAGTCGCGCGCGTTCGCCTACGGCA
TCATGAACGAGGAGCAGCGCCGGCAGTTCGAGGAGCACAGCGAGTGCAACTTCGCGATCGCGCCCCAGGAGATCGGACGC
TTTCGCGTCAACGTCTTCATGCAGCAGCAGCGCGTCGGCATGGTGCTGCGCACGATCAACACCGACATCCCGCGCTTCGA
CGACCTGAACCTGCCCAAGGTGCTGGCCGACGTGGCGCTCACGAAGCGCGGTCTCGTGCTCTTCGTCGGCGGCACGGGCT
CGGGCAAATCGACCTCGCTCGCGGCGATGATCGGCTACCGCAACGAGTACAGCTACGGCCACATCCTCACGGTCGAGGAC
CCGATCGAGTACGTGCACCAGCACAAGAACTGCATCATCACGCAGCGCGAGGTGGGGGTGGACACCGAGTCCTACGAGGT
GGCGCTCAAGAACGCGATGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAGATCCGCTCGCGCGAGGCCATGGACCTCG
GCGTGCAGTTCGCGGAGACCGGTCACCTGTGCATGTCGACCCTGCACGCGAACAACGCGAACCAGGCCCTGGACCGCATC
ATCAACTTCTTTCCCGAGGAGAAGCGCAACCAGCTGCTCATGGACCTCGCGCTCAACCTCAAGGCGATCATCTCGCAGCG
CCTGATCCCCACGAAGAGCGGCAAGGGCCGCGTGCCGGCGGTCGAGGTACTCATCAACACTCCGCTCGTGTCCGACATGA
TCATGGAAGGCAACATCCCGGGCCTGAAGGACCTCATGGCGAAGTCGCCCGAGGCGGGCATGCAGACGTTCGACCAGTCG
CTGTTCACCCTCTTCGAGGCCGACCTCATCTCCTACGACGACGCCCTGCGCAACGCCGACTCGGTCAACGACGTGCGCCT
GCGCATCAAGCTCGAGAGCAAGGACTCCAAGAACCGCGAGCTGGGCGACACGCTTCGGAACGTGACGTACAAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4VBU0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.254

95.418

0.623
  pilU Acinetobacter baylyi ADP1

57.067

100

0.577
  pilU Vibrio cholerae strain A1552

55.908

93.531

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.133

91.375

0.412
  pilT Acinetobacter baylyi ADP1

42.522

91.914

0.391
  pilT Legionella pneumophila strain Lp02

42.388

90.296

0.383
  pilT Legionella pneumophila strain ERS1305867

42.388

90.296

0.383
  pilT Acinetobacter nosocomialis M2

42.598

89.218

0.38
  pilT Acinetobacter baumannii D1279779

42.598

89.218

0.38
  pilT Acinetobacter baumannii strain A118

42.598

89.218

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.298

91.375

0.377
  pilT Pseudomonas aeruginosa PAK

41.298

91.375

0.377

Multiple sequence alignment