Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SCL_RS12095 Genome accession   NZ_AP014879
Coordinates   2502429..2503505 (-) Length   358 a.a.
NCBI ID   WP_096361438.1    Uniprot ID   A0A1B4XIV8
Organism   Sulfuricaulis limicola strain HA5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2497429..2508505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCL_RS12065 (SCL_2448) - 2497439..2498002 (+) 564 WP_096361434.1 YqgE/AlgH family protein -
  SCL_RS12070 (SCL_2449) ruvX 2498002..2498433 (+) 432 WP_096361435.1 Holliday junction resolvase RuvX -
  SCL_RS12075 (SCL_2450) pyrR 2498469..2498927 (+) 459 WP_096361978.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  SCL_RS12080 (SCL_2451) - 2498924..2499946 (+) 1023 WP_197702628.1 aspartate carbamoyltransferase catalytic subunit -
  SCL_RS12085 (SCL_2452) - 2499943..2501229 (+) 1287 WP_096361436.1 dihydroorotase -
  SCL_RS12090 (SCL_2453) pilU 2501317..2502432 (-) 1116 WP_096361437.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SCL_RS12095 (SCL_2454) pilT 2502429..2503505 (-) 1077 WP_096361438.1 type IV pilus twitching motility protein PilT Machinery gene
  SCL_RS12100 (SCL_2455) proC 2503705..2504556 (+) 852 WP_096361439.1 pyrroline-5-carboxylate reductase -
  SCL_RS12105 (SCL_2456) - 2504553..2505113 (+) 561 WP_096361440.1 YggT family protein -
  SCL_RS12110 (SCL_2457) - 2505115..2505405 (+) 291 WP_096361441.1 DUF167 family protein -
  SCL_RS12115 (SCL_2458) - 2505539..2507512 (+) 1974 WP_096361442.1 dynamin family protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39200.82 Da        Isoelectric Point: 6.5042

>NTDB_id=67227 SCL_RS12095 WP_096361438.1 2502429..2503505(-) (pilT) [Sulfuricaulis limicola strain HA5]
MDIAELLAFAFKQNASDLHLSAGLPPLIRVDGDIKRINVDPLDDRTVHNMVYDIMSDKQQKDYEEFLETDFSFELPNIAR
FRVNAFNQNRGPAAVFRTIPSKVLTLEQLNCPPVFKQIADQPRGIVLVTGPTGSGKSTTLAAMVNHINETEHGHILTIED
PIEFVHTSKNCLINQREIGKDTLGFNNALRSALREDPDVILVGEMRDLETIRLALTGAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRAMLSESLRAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNVGMSTLDQNL
TDLIRTRQVHVDDARAVAANKDLFSSAAAVASSGGGKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=67227 SCL_RS12095 WP_096361438.1 2502429..2503505(-) (pilT) [Sulfuricaulis limicola strain HA5]
ATGGATATCGCCGAACTGCTGGCGTTTGCCTTCAAACAGAACGCCTCGGATCTGCATTTGTCGGCCGGGCTGCCACCGCT
GATTCGCGTGGACGGTGACATCAAGCGCATCAACGTGGACCCGCTGGACGACCGCACCGTTCACAACATGGTCTACGACA
TCATGTCGGACAAGCAGCAGAAGGACTACGAAGAATTCCTCGAGACCGACTTCTCCTTCGAGCTGCCCAATATCGCGCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCCCGGCGGCAGTGTTCCGCACCATTCCCTCGAAGGTGCTGACGCTCGA
GCAGCTCAATTGCCCGCCGGTGTTCAAGCAGATCGCCGACCAGCCGCGCGGGATCGTGCTCGTGACAGGACCCACCGGCT
CCGGCAAGTCCACGACCCTGGCGGCCATGGTCAACCACATCAACGAAACCGAGCACGGACATATTCTCACCATCGAGGAC
CCGATCGAATTCGTGCACACCAGCAAGAATTGCCTGATCAACCAGCGCGAGATCGGCAAGGACACGCTCGGCTTCAACAA
CGCCCTGCGCTCTGCCCTGCGCGAGGACCCGGACGTGATTCTGGTGGGCGAAATGCGCGACCTGGAAACGATCCGCCTGG
CGTTGACCGGCGCCGAGACCGGTCACCTGGTTTTCGGAACCCTGCATACGAGCTCCGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCGGCGGAAAAGGACATGGTGCGCGCCATGCTTTCCGAGTCCTTGCGCGCAGTCATCTCGCAGAC
CCTGCTCAAGAAGATCGGCGGCGGCCGCGTGGCGGCGCACGAGATCATGATCGGCACGCCCGCGATCCGCAACCTGATTC
GCGAGAACAAGATCGCGCAGATGTATTCGGCCATCCAGACCGGTCAGAATGTGGGCATGTCCACGCTCGACCAGAATCTG
ACGGATCTGATACGCACGCGCCAGGTGCATGTGGATGACGCCCGTGCCGTGGCCGCCAACAAGGACCTGTTCAGTTCCGC
GGCAGCGGTGGCGTCAAGCGGCGGTGGCAAGAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XIV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

75.581

96.089

0.726
  pilT Acinetobacter baumannii D1279779

75.581

96.089

0.726
  pilT Acinetobacter baumannii strain A118

75.581

96.089

0.726
  pilT Pseudomonas aeruginosa PAK

75.148

94.413

0.709
  pilT Acinetobacter baylyi ADP1

73.837

96.089

0.709
  pilT Pseudomonas stutzeri DSM 10701

73.547

96.089

0.707
  pilT Legionella pneumophila strain Lp02

69.767

96.089

0.67
  pilT Legionella pneumophila strain ERS1305867

69.767

96.089

0.67
  pilT Neisseria meningitidis 8013

67.63

96.648

0.654
  pilT Neisseria gonorrhoeae MS11

67.341

96.648

0.651
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.753

90.503

0.631
  pilT Vibrio cholerae strain A1552

69.753

90.503

0.631
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.7

99.721

0.506
  pilU Acinetobacter baylyi ADP1

41.071

93.855

0.385
  pilU Vibrio cholerae strain A1552

40.299

93.575

0.377
  pilU Pseudomonas stutzeri DSM 10701

40

93.575

0.374

Multiple sequence alignment