Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SCL_RS12090 Genome accession   NZ_AP014879
Coordinates   2501317..2502432 (-) Length   371 a.a.
NCBI ID   WP_096361437.1    Uniprot ID   -
Organism   Sulfuricaulis limicola strain HA5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2496317..2507432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCL_RS12060 (SCL_2447) - 2496507..2497394 (+) 888 WP_096361433.1 energy transducer TonB -
  SCL_RS12065 (SCL_2448) - 2497439..2498002 (+) 564 WP_096361434.1 YqgE/AlgH family protein -
  SCL_RS12070 (SCL_2449) ruvX 2498002..2498433 (+) 432 WP_096361435.1 Holliday junction resolvase RuvX -
  SCL_RS12075 (SCL_2450) pyrR 2498469..2498927 (+) 459 WP_096361978.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  SCL_RS12080 (SCL_2451) - 2498924..2499946 (+) 1023 WP_197702628.1 aspartate carbamoyltransferase catalytic subunit -
  SCL_RS12085 (SCL_2452) - 2499943..2501229 (+) 1287 WP_096361436.1 dihydroorotase -
  SCL_RS12090 (SCL_2453) pilU 2501317..2502432 (-) 1116 WP_096361437.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SCL_RS12095 (SCL_2454) pilT 2502429..2503505 (-) 1077 WP_096361438.1 type IV pilus twitching motility protein PilT Machinery gene
  SCL_RS12100 (SCL_2455) proC 2503705..2504556 (+) 852 WP_096361439.1 pyrroline-5-carboxylate reductase -
  SCL_RS12105 (SCL_2456) - 2504553..2505113 (+) 561 WP_096361440.1 YggT family protein -
  SCL_RS12110 (SCL_2457) - 2505115..2505405 (+) 291 WP_096361441.1 DUF167 family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41497.85 Da        Isoelectric Point: 6.9126

>NTDB_id=67226 SCL_RS12090 WP_096361437.1 2501317..2502432(-) (pilU) [Sulfuricaulis limicola strain HA5]
MNFIDLLKLMKHKHASDLFVTAGVAPSIKVDGKIMPVTKQALTPEQSRAFAYGIMNEDQRRQFEASNECNFAIAPQEIGR
FRVNVFMQQQRVGMVLRTINTDIPRFDDLHLPKVLADLALTKRGLILFVGGTGSGKSTSLAAMIGYRNEYSYGHILTIED
PVEYVHDHKNCIVNQREVGVDTSSYEVALKNAMRQAPDVILIGEIRARETMDLAIQFSETGHLCLSTLHANNANQALDRI
INFFPEDKRNQLLMDLGLNLKAIVSQRLIPMKSGKGRVPAVEVMINSPLIADLIMEGKIPEIKDLMGKSTEAGMQTFDQS
LFSLYEADLISYDDALRNADSVNDVRLRIKLEGKDSKSRELGESMRNVTIK

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=67226 SCL_RS12090 WP_096361437.1 2501317..2502432(-) (pilU) [Sulfuricaulis limicola strain HA5]
ATGAATTTCATCGACCTGCTCAAGCTCATGAAGCACAAGCACGCCTCCGACTTGTTCGTCACGGCCGGGGTGGCGCCCTC
CATCAAGGTGGACGGCAAGATCATGCCGGTGACGAAGCAGGCGCTGACCCCGGAGCAGTCGCGCGCCTTCGCCTACGGCA
TCATGAACGAGGACCAGCGCCGGCAGTTCGAGGCGAGCAACGAATGCAATTTCGCGATCGCGCCGCAGGAGATCGGCCGT
TTCCGCGTCAATGTATTTATGCAGCAGCAGCGCGTGGGCATGGTGTTGCGCACCATCAACACCGACATCCCGCGTTTTGA
CGACCTGCATCTGCCCAAGGTGCTGGCGGACCTGGCGCTGACCAAGCGCGGGCTCATCCTGTTCGTCGGCGGCACCGGTT
CGGGCAAATCCACCTCGCTGGCAGCCATGATCGGCTATCGCAACGAGTACAGCTACGGGCACATCCTCACCATCGAGGAC
CCGGTGGAATACGTGCACGACCACAAGAACTGCATCGTGAACCAGCGCGAGGTCGGTGTCGACACGTCGTCCTACGAAGT
GGCGCTGAAAAACGCCATGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAAATCCGTGCACGCGAAACCATGGACCTGG
CGATCCAGTTTTCCGAAACCGGCCACTTGTGCCTGTCCACCCTGCACGCCAACAACGCCAACCAGGCACTCGACCGCATC
ATCAACTTCTTCCCCGAGGACAAGCGCAACCAGCTGCTGATGGACCTGGGGCTCAACCTGAAGGCCATTGTTTCGCAGCG
CCTGATCCCGATGAAGAGCGGCAAGGGGCGTGTACCCGCCGTGGAGGTCATGATCAACTCGCCCCTGATCGCCGACCTGA
TCATGGAAGGCAAGATCCCGGAGATCAAGGATCTCATGGGCAAGTCCACCGAGGCCGGCATGCAGACCTTCGACCAGTCG
CTGTTCAGCCTGTACGAGGCCGACCTCATTTCCTATGACGATGCGCTGCGCAATGCCGACTCGGTCAACGACGTGCGCCT
GCGCATCAAGCTCGAAGGCAAGGACTCGAAGAGTCGCGAGCTCGGTGAGTCCATGCGTAACGTGACGATCAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-249)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.534

100

0.625
  pilU Acinetobacter baylyi ADP1

56.831

98.652

0.561
  pilU Vibrio cholerae strain A1552

53.444

97.844

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.658

91.375

0.399
  pilT Acinetobacter baylyi ADP1

42.229

91.914

0.388
  pilT Pseudomonas stutzeri DSM 10701

42.183

91.375

0.385
  pilT Pseudomonas aeruginosa PAK

41.888

91.375

0.383
  pilT Legionella pneumophila strain ERS1305867

41.791

90.296

0.377
  pilT Legionella pneumophila strain Lp02

41.791

90.296

0.377
  pilT Acinetobacter baumannii D1279779

42.296

89.218

0.377
  pilT Acinetobacter nosocomialis M2

42.296

89.218

0.377
  pilT Acinetobacter baumannii strain A118

42.296

89.218

0.377
  pilT Neisseria gonorrhoeae MS11

39.528

91.375

0.361
  pilT Neisseria meningitidis 8013

39.528

91.375

0.361

Multiple sequence alignment