Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KF707C_RS01330 Genome accession   NZ_AP014862
Coordinates   280531..281565 (+) Length   344 a.a.
NCBI ID   WP_003455638.1    Uniprot ID   A0AAD1FDG2
Organism   Metapseudomonas furukawaii strain KF707     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 275531..286565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KF707C_RS01305 (KF707C_2590) - 275735..277723 (-) 1989 WP_003455643.1 dynamin-like GTPase family protein -
  KF707C_RS01310 (KF707C_2600) - 277939..278235 (-) 297 WP_003455642.1 DUF167 domain-containing protein -
  KF707C_RS01315 (KF707C_2610) - 278232..278825 (-) 594 WP_003455641.1 YggT family protein -
  KF707C_RS01320 (KF707C_2620) proC 278840..279658 (-) 819 WP_003455640.1 pyrroline-5-carboxylate reductase -
  KF707C_RS01325 (KF707C_2630) - 279673..280365 (-) 693 WP_003455639.1 YggS family pyridoxal phosphate-dependent enzyme -
  KF707C_RS01330 (KF707C_2640) pilT 280531..281565 (+) 1035 WP_003455638.1 type IV pilus twitching motility protein PilT Machinery gene
  KF707C_RS01335 (KF707C_2650) pilU 281605..282750 (+) 1146 WP_003455637.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KF707C_RS01340 (KF707C_2660) - 282855..283250 (+) 396 WP_003455636.1 TM2 domain-containing protein -
  KF707C_RS01345 (KF707C_2670) - 283358..283681 (-) 324 WP_003455635.1 hypothetical protein -
  KF707C_RS01350 - 284163..284384 (-) 222 WP_081608126.1 hypothetical protein -
  KF707C_RS01355 - 284374..285051 (-) 678 Protein_259 RHS repeat-associated core domain-containing protein -
  KF707C_RS01360 - 285451..285816 (-) 366 WP_081608124.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37957.74 Da        Isoelectric Point: 6.8360

>NTDB_id=67131 KF707C_RS01330 WP_003455638.1 280531..281565(+) (pilT) [Metapseudomonas furukawaii strain KF707]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEIFKKVSDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSTKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KSLVAKGLVSRESAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=67131 KF707C_RS01330 WP_003455638.1 280531..281565(+) (pilT) [Metapseudomonas furukawaii strain KF707]
ATGGACATTACCGAGCTGCTGGCCTTCAGTGCCAAGCAGGGCGCTTCGGACCTGCATCTCTCTGCCGGCCTGCCGCCCAT
GATTCGCGTCGACGGCGACGTCCGCCGGATCAACCTGCCGCCGCTGGATCACAAGCAGGTTCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAGTTCCTGGAGACCGACTTCTCCTTCGAAGTCCCCGGCGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGTGCGGGCGCGGTGTTCCGGACCATTCCCTCCAAGGTCCTCACCATGGA
AGACCTGGGCATGGGCGAGATCTTCAAGAAGGTCTCCGACGTACCCCGTGGCCTGGTGCTGGTCACCGGGCCCACCGGTT
CGGGCAAGTCCACCACGCTGGCAGCCATGCTCGACTACCTGAACAGCACCAAGTACCACCATATCCTCACCGTGGAAGAC
CCCATCGAGTTCGTCCACGAGTCCAAGAAGTGCCTGATCAACCAGCGGGAAGTCCATCGCGACACCCTGGGCTTCAACGA
GGCCCTGCGCTCGGCCCTGCGTGAGGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAGACCATCCGTCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGGGTC
GTCGACGTGTTCCCGGCCGAGGAGAAGTCCATGGTGCGCTCCATGCTGTCCGAATCCCTCCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGGGTAGCGGCCCACGAGATCATGATTGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCCGCCATCCAGACCGGCGGCTCTCTCGGCATGCAGACTCTCGACATGTGCCTC
AAGAGCCTGGTGGCCAAGGGACTGGTCAGCCGCGAAAGCGCGCGGGAAAAGGCCAAGAGCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.349

100

0.953
  pilT Pseudomonas stutzeri DSM 10701

93.895

100

0.939
  pilT Acinetobacter baumannii D1279779

85.756

100

0.858
  pilT Acinetobacter baumannii strain A118

85.756

100

0.858
  pilT Acinetobacter nosocomialis M2

85.465

100

0.855
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

76.331

98.256

0.75
  pilT Legionella pneumophila strain ERS1305867

76.331

98.256

0.75
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.71

100

0.398

Multiple sequence alignment