Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KF707C_RS01335 Genome accession   NZ_AP014862
Coordinates   281605..282750 (+) Length   381 a.a.
NCBI ID   WP_003455637.1    Uniprot ID   A0AAD1FDQ7
Organism   Metapseudomonas furukawaii strain KF707     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 276605..287750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KF707C_RS01310 (KF707C_2600) - 277939..278235 (-) 297 WP_003455642.1 DUF167 domain-containing protein -
  KF707C_RS01315 (KF707C_2610) - 278232..278825 (-) 594 WP_003455641.1 YggT family protein -
  KF707C_RS01320 (KF707C_2620) proC 278840..279658 (-) 819 WP_003455640.1 pyrroline-5-carboxylate reductase -
  KF707C_RS01325 (KF707C_2630) - 279673..280365 (-) 693 WP_003455639.1 YggS family pyridoxal phosphate-dependent enzyme -
  KF707C_RS01330 (KF707C_2640) pilT 280531..281565 (+) 1035 WP_003455638.1 type IV pilus twitching motility protein PilT Machinery gene
  KF707C_RS01335 (KF707C_2650) pilU 281605..282750 (+) 1146 WP_003455637.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KF707C_RS01340 (KF707C_2660) - 282855..283250 (+) 396 WP_003455636.1 TM2 domain-containing protein -
  KF707C_RS01345 (KF707C_2670) - 283358..283681 (-) 324 WP_003455635.1 hypothetical protein -
  KF707C_RS01350 - 284163..284384 (-) 222 WP_081608126.1 hypothetical protein -
  KF707C_RS01355 - 284374..285051 (-) 678 Protein_259 RHS repeat-associated core domain-containing protein -
  KF707C_RS01360 - 285451..285816 (-) 366 WP_081608124.1 hypothetical protein -
  KF707C_RS01365 - 285825..286721 (-) 897 WP_051050776.1 RHS repeat domain-containing protein -
  KF707C_RS01370 - 286734..287081 (-) 348 WP_036993800.1 hypothetical protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42624.00 Da        Isoelectric Point: 6.8267

>NTDB_id=67132 KF707C_RS01335 WP_003455637.1 281605..282750(+) (pilU) [Metapseudomonas furukawaii strain KF707]
MEFEKLLRLMVEKGASDLFVTAGVPPSMKVNGKIMPVSKNAMSPEMTRETVLGVMNEQQRRDFAQHHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETSIPTIDELKLPEVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGHRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGLDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAIAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYNLYAQGEITYEDALLHADSANDLRLMIKLDSETDGEHLQHMSEGLSLQVSEEDPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=67132 KF707C_RS01335 WP_003455637.1 281605..282750(+) (pilU) [Metapseudomonas furukawaii strain KF707]
ATGGAATTCGAGAAACTGCTGCGCCTGATGGTGGAAAAGGGAGCGTCCGACCTGTTCGTCACGGCCGGTGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTCAGCAAGAACGCCATGTCGCCGGAAATGACCCGTGAGACGGTGCTGGGCG
TGATGAACGAGCAGCAGCGTCGCGACTTCGCCCAGCACCACGAATGCAACTTCGCCATCAGCGCCCGGGGCATTGGCCGC
TTCCGTGTGAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACTTCCATCCCCACCATCGA
TGAGCTGAAGCTGCCGGAGGTCCTGAAGAAGCTGGCGCTGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCCACCGCAACAAGAACAGCAGCGGGCACATCATCTCCATCGAGGAC
CCCATCGAGTTCATCCACCAGCACCAGAACTGCATCGTCACCCAGCGCGAGGTGGGACTGGACACCGAGTCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGGTGCGGACCCGCGAAACCATGGACCACG
CCATCGCCTTCGCCGAGACCGGCCACCTTTGCCTCGCCACCCTCCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGCCGACCGGCAGCAACAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCGGCGGACCTGA
TCCGCAAGGGTGAGGTGCACGAGCTCAAGCCGTTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTCTACAACCTCTATGCCCAGGGCGAGATCACCTACGAGGACGCGCTGCTCCACGCCGATTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGACTCGGAGACCGACGGCGAACACCTGCAGCACATGAGCGAAGGGCTGTCGCTGCAGGTGTCCGAAG
AGGACCCGGGGCGTCGGTTCCGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(110-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

90.289

100

0.903
  pilU Acinetobacter baylyi ADP1

64.706

93.701

0.606
  pilU Vibrio cholerae strain A1552

59.714

91.864

0.549
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

88.976

0.391

Multiple sequence alignment