Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ISX51_RS03565 Genome accession   NZ_CP094659
Coordinates   713212..714321 (+) Length   369 a.a.
NCBI ID   WP_281053353.1    Uniprot ID   -
Organism   Vibrio lentus strain ED252     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 708212..719321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISX51_RS03530 (ISX51_03530) - 708448..709047 (-) 600 WP_281053350.1 XTP/dITP diphosphatase -
  ISX51_RS03535 (ISX51_03535) - 709096..709527 (-) 432 WP_281053351.1 DUF4426 domain-containing protein -
  ISX51_RS03540 (ISX51_03540) yggU 709556..709846 (-) 291 WP_009847703.1 DUF167 family protein YggU -
  ISX51_RS03545 (ISX51_03545) - 709876..710433 (-) 558 WP_017067798.1 YggT family protein -
  ISX51_RS03550 (ISX51_03550) proC 710510..711328 (-) 819 WP_102279313.1 pyrroline-5-carboxylate reductase -
  ISX51_RS03555 (ISX51_03555) - 711429..712133 (-) 705 WP_281053352.1 YggS family pyridoxal phosphate-dependent enzyme -
  ISX51_RS03560 (ISX51_03560) pilT 712160..713197 (+) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  ISX51_RS03565 (ISX51_03565) pilU 713212..714321 (+) 1110 WP_281053353.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ISX51_RS03570 (ISX51_03570) - 714405..714566 (-) 162 WP_170960663.1 hypothetical protein -
  ISX51_RS03575 (ISX51_03575) ruvX 714666..715088 (-) 423 WP_017062240.1 Holliday junction resolvase RuvX -
  ISX51_RS03580 (ISX51_03580) - 715149..715712 (-) 564 WP_054547449.1 YqgE/AlgH family protein -
  ISX51_RS03585 (ISX51_03585) gshB 715828..716778 (-) 951 WP_281053354.1 glutathione synthase -
  ISX51_RS03590 (ISX51_03590) rsmE 716788..717519 (-) 732 WP_017077778.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ISX51_RS03595 (ISX51_03595) - 717679..718410 (-) 732 WP_281053355.1 endonuclease -
  ISX51_RS03600 (ISX51_03600) - 718508..719008 (-) 501 WP_017097525.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41385.22 Da        Isoelectric Point: 5.8676

>NTDB_id=670473 ISX51_RS03565 WP_281053353.1 713212..714321(+) (pilU) [Vibrio lentus strain ED252]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNSVQVSQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGDLHELKATMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALSGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=670473 ISX51_RS03565 WP_281053353.1 713212..714321(+) (pilU) [Vibrio lentus strain ED252]
ATGGAATTGAATCAAATCCTTGAGGGGATGCTTTCTCAAAAGGCTTCGGATCTTTATATCACTGTCGATGCTCCAATCCT
GTTTCGTGTGGATGGTGAACTACGACCTCAAGGAGAGAAACTGAATTCGGTTCAAGTATCTCAGCTTCTTGATGCGATGA
TGGATCAAGACCGACGTGATGAATATCAGCAAACGCGTGAGGCTAATTTTGCCATCGTACGTGACTTTGGTCGTTTTCGT
GTCAGTGCATTCTTTCAACGAGAGCTACCTGGAGCTGTCATTCGACGTATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTGCTGCAAGACCTTTCAATCGCTAAGCGCGGACTTGTGCTGGTGGTTGGTGCAACAGGTTCTGGTA
AATCAACCTCGATGGCCGCAATGACGGGTTATCGTAATACTAATCGTTCAGGGCATATTTTGACGGTTGAAGACCCAATT
GAATTCGTTCACGAACATAAAAAGTGCATCGTGACTCAACGTGAGGTTGGGCTAGACACTGATAGTTATGAAGTTGCGCT
TAAGAACTCTTTGCGGCAAGCACCTGATATGATTTTGATTGGCGAAATCCGTAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGCCATTTGTGCATGGCAACACTTCACGCGAATAATGCCAACCAAGCGTTAGAACGCATTCTTCAC
TTGGTGCCGAAAGAACAGAAAGAGCAGTTCCTGTTTGACCTGTCGATGAACCTGCGTGGTGTGGTCGCTCAGCAATTAAT
CCGAGATAAGAATGGCAGCGGTCGTCATGGTGTGTTTGAGATTCTACTCAACAGCCCGCGAGTGTCTGACTTGATTCGTC
GTGGTGACTTGCATGAGCTTAAAGCGACTATGGCTAAATCGAAAGAGATTGGGATGCAGACCTTTGACCAAGCTTTGTAT
GATTTAGTCGTTGCTGGCAAGATTAGCGAAGAAGATGCGTTCCACAGTGCAGATTCAGCCAATGACTTGCGCTTGATGTT
GAAAACCAGACGTGGCGATGATGACTACGGGACTGGCGCTTTGTCTGGCGTTAAGATTGATATGGGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.967

99.729

0.837
  pilU Pseudomonas stutzeri DSM 10701

56.604

100

0.569
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Legionella pneumophila strain Lp02

42.398

92.683

0.393
  pilT Legionella pneumophila strain ERS1305867

42.398

92.683

0.393
  pilT Acinetobacter baumannii strain A118

42.353

92.141

0.39
  pilT Acinetobacter baumannii D1279779

42.353

92.141

0.39
  pilT Acinetobacter nosocomialis M2

42.353

92.141

0.39
  pilT Acinetobacter baylyi ADP1

41.813

92.683

0.388
  pilT Pseudomonas aeruginosa PAK

41.593

91.87

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.901

87.805

0.377
  pilT Pseudomonas stutzeri DSM 10701

40.118

91.87

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.551

93.496

0.36
  pilT Neisseria meningitidis 8013

38.551

93.496

0.36