Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ISX51_RS03560 Genome accession   NZ_CP094659
Coordinates   712160..713197 (+) Length   345 a.a.
NCBI ID   WP_004735478.1    Uniprot ID   A0A1R3EAA6
Organism   Vibrio lentus strain ED252     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 707160..718197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISX51_RS03525 (ISX51_03525) hemW 707261..708448 (-) 1188 WP_281053349.1 radical SAM family heme chaperone HemW -
  ISX51_RS03530 (ISX51_03530) - 708448..709047 (-) 600 WP_281053350.1 XTP/dITP diphosphatase -
  ISX51_RS03535 (ISX51_03535) - 709096..709527 (-) 432 WP_281053351.1 DUF4426 domain-containing protein -
  ISX51_RS03540 (ISX51_03540) yggU 709556..709846 (-) 291 WP_009847703.1 DUF167 family protein YggU -
  ISX51_RS03545 (ISX51_03545) - 709876..710433 (-) 558 WP_017067798.1 YggT family protein -
  ISX51_RS03550 (ISX51_03550) proC 710510..711328 (-) 819 WP_102279313.1 pyrroline-5-carboxylate reductase -
  ISX51_RS03555 (ISX51_03555) - 711429..712133 (-) 705 WP_281053352.1 YggS family pyridoxal phosphate-dependent enzyme -
  ISX51_RS03560 (ISX51_03560) pilT 712160..713197 (+) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  ISX51_RS03565 (ISX51_03565) pilU 713212..714321 (+) 1110 WP_281053353.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ISX51_RS03570 (ISX51_03570) - 714405..714566 (-) 162 WP_170960663.1 hypothetical protein -
  ISX51_RS03575 (ISX51_03575) ruvX 714666..715088 (-) 423 WP_017062240.1 Holliday junction resolvase RuvX -
  ISX51_RS03580 (ISX51_03580) - 715149..715712 (-) 564 WP_054547449.1 YqgE/AlgH family protein -
  ISX51_RS03585 (ISX51_03585) gshB 715828..716778 (-) 951 WP_281053354.1 glutathione synthase -
  ISX51_RS03590 (ISX51_03590) rsmE 716788..717519 (-) 732 WP_017077778.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38317.83 Da        Isoelectric Point: 6.0512

>NTDB_id=670472 ISX51_RS03560 WP_004735478.1 712160..713197(+) (pilT) [Vibrio lentus strain ED252]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPEIFERISNYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=670472 ISX51_RS03560 WP_004735478.1 712160..713197(+) (pilT) [Vibrio lentus strain ED252]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGCGGTGAGTTTGAAGAAAAACTGGAAGTAGACTTCTCTTTTGAATTACCCAACGTTGGTCGT
TTCCGTGTTAATGCTTTTAACCAATCTCGTGGCTGCTCTGCCGTATTTCGTACCATTCCAGTAGAGATTCCGACCCTTGA
ACAGCTAGGGGCTCCTGAAATCTTTGAAAGAATATCTAATTACGAAAAAGGTTTAGTCTTGGTGACAGGGCCGACGGGTT
CGGGTAAGTCGACAACTTTGGCTGCGATGGTGGATTACGTTAACCGAAACCATAATAAGCATATCTTGACGATTGAAGAC
CCGATCGAATTTGTTCATACCAACAACAAATGCCTAATCAACCAACGAGAAGTGCACCGCGATACTCACAGCTTCAAAGC
TGCGCTGCGCAGTGCGTTACGTGAAGATCCAGACGTTATCCTTGTTGGTGAGCTTCGTGACCAAGAGACGATCAGCTTAG
CGCTAACCGCAGCAGAAACGGGTCACTTAGTGTTTGGTACCTTACACACGAGCTCTGCGGCAAAAACTATCGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGATATGGTGCGTTCAATGTTGTCTGAATCGTTACGTTCGGTGATCGCCCAGAA
GCTGTTAAAGCGTGTCGGTGGTGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCGATTAGAAACTTGATCC
GTGAAGATAAGGTCGCGCAGATGTATTCGATTATTCAAACGGGCGCGGCACACGGTATGCAAACCATGGAACAAAATGCG
AAGCAGCTGATGGCTCAAGGCTTGGTTGATTCAGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R3EAA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Pseudomonas aeruginosa PAK

69.412

98.551

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368